Sperm chromatin-induced ectopic polar body extrusion in mouse eggs after ICSI and delayed egg activation.

Meiotic chromosomes in an oocyte are not only a maternal genome carrier but also provide a positional signal to induce cortical polarization and define asymmetric meiotic division of the oocyte, resulting in polar body extrusion and haploidization of the maternal genome. The meiotic chromosomes play...

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Main Authors: Manqi Deng, Rong Li
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2009-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2746308?pdf=render
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author Manqi Deng
Rong Li
author_facet Manqi Deng
Rong Li
author_sort Manqi Deng
collection DOAJ
description Meiotic chromosomes in an oocyte are not only a maternal genome carrier but also provide a positional signal to induce cortical polarization and define asymmetric meiotic division of the oocyte, resulting in polar body extrusion and haploidization of the maternal genome. The meiotic chromosomes play dual function in determination of meiosis: 1) organizing a bipolar spindle formation and 2) inducing cortical polarization and assembly of a distinct cortical cytoskeleton structure in the overlying cortex for polar body extrusion. At fertilization, a sperm brings exogenous paternal chromatin into the egg, which induces ectopic cortical polarization at the sperm entry site and leads to a cone formation, known as fertilization cone. Here we show that the sperm chromatin-induced fertilization cone formation is an abortive polar body extrusion due to lack of spindle induction by the sperm chromatin during fertilization. If experimentally manipulating the fertilization process to allow sperm chromatin to induce both cortical polarization and spindle formation, the fertilization cone can be converted into polar body extrusion. This suggests that sperm chromatin is also able to induce polar body extrusion, like its maternal counterpart. The usually observed cone formation instead of ectopic polar body extrusion induced by sperm chromatin during fertilization is due to special sperm chromatin compaction which restrains it from rapid spindle induction and therefore provides a protective mechanism to prevent a possible paternal genome loss during ectopic polar body extrusion.
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spelling doaj.art-6f10f425edb44df6b381fa4d61d2aec42022-12-22T00:46:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-01-0149e717110.1371/journal.pone.0007171Sperm chromatin-induced ectopic polar body extrusion in mouse eggs after ICSI and delayed egg activation.Manqi DengRong LiMeiotic chromosomes in an oocyte are not only a maternal genome carrier but also provide a positional signal to induce cortical polarization and define asymmetric meiotic division of the oocyte, resulting in polar body extrusion and haploidization of the maternal genome. The meiotic chromosomes play dual function in determination of meiosis: 1) organizing a bipolar spindle formation and 2) inducing cortical polarization and assembly of a distinct cortical cytoskeleton structure in the overlying cortex for polar body extrusion. At fertilization, a sperm brings exogenous paternal chromatin into the egg, which induces ectopic cortical polarization at the sperm entry site and leads to a cone formation, known as fertilization cone. Here we show that the sperm chromatin-induced fertilization cone formation is an abortive polar body extrusion due to lack of spindle induction by the sperm chromatin during fertilization. If experimentally manipulating the fertilization process to allow sperm chromatin to induce both cortical polarization and spindle formation, the fertilization cone can be converted into polar body extrusion. This suggests that sperm chromatin is also able to induce polar body extrusion, like its maternal counterpart. The usually observed cone formation instead of ectopic polar body extrusion induced by sperm chromatin during fertilization is due to special sperm chromatin compaction which restrains it from rapid spindle induction and therefore provides a protective mechanism to prevent a possible paternal genome loss during ectopic polar body extrusion.http://europepmc.org/articles/PMC2746308?pdf=render
spellingShingle Manqi Deng
Rong Li
Sperm chromatin-induced ectopic polar body extrusion in mouse eggs after ICSI and delayed egg activation.
PLoS ONE
title Sperm chromatin-induced ectopic polar body extrusion in mouse eggs after ICSI and delayed egg activation.
title_full Sperm chromatin-induced ectopic polar body extrusion in mouse eggs after ICSI and delayed egg activation.
title_fullStr Sperm chromatin-induced ectopic polar body extrusion in mouse eggs after ICSI and delayed egg activation.
title_full_unstemmed Sperm chromatin-induced ectopic polar body extrusion in mouse eggs after ICSI and delayed egg activation.
title_short Sperm chromatin-induced ectopic polar body extrusion in mouse eggs after ICSI and delayed egg activation.
title_sort sperm chromatin induced ectopic polar body extrusion in mouse eggs after icsi and delayed egg activation
url http://europepmc.org/articles/PMC2746308?pdf=render
work_keys_str_mv AT manqideng spermchromatininducedectopicpolarbodyextrusioninmouseeggsaftericsianddelayedeggactivation
AT rongli spermchromatininducedectopicpolarbodyextrusioninmouseeggsaftericsianddelayedeggactivation