Effects of elastase-induced emphysema on muscle and bone in mice.
Chronic obstructive pulmonary disease (COPD) causes sarcopenia and osteoporosis. However, the mechanisms underlying muscle and bone loss as well as the interactions between muscle and bone in the COPD state remain unclear. Therefore, we herein investigated the effects of the COPD state on muscle and...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0287541 |
| _version_ | 1797394481522147328 |
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| author | Daichi Matsumura Naoyuki Kawao Katsumi Okumoto Takashi Ohira Yuya Mizukami Masao Akagi Hiroshi Kaji |
| author_facet | Daichi Matsumura Naoyuki Kawao Katsumi Okumoto Takashi Ohira Yuya Mizukami Masao Akagi Hiroshi Kaji |
| author_sort | Daichi Matsumura |
| collection | DOAJ |
| description | Chronic obstructive pulmonary disease (COPD) causes sarcopenia and osteoporosis. However, the mechanisms underlying muscle and bone loss as well as the interactions between muscle and bone in the COPD state remain unclear. Therefore, we herein investigated the effects of the COPD state on muscle and bone in mice intratracheally administered porcine pancreatic elastase (PPE). The intratracheal administration of PPE to mice significantly reduced trabecular bone mineral density (BMD), trabecular bone volume, trabecular number, cortical BMD and cortical area. It also significantly decreased grip strength, but did not affect muscle mass or the expression of myogenic differentiation-, protein degradation- or autophagy-related genes in the soleus and gastrocnemius muscles. Among the myokines examined, myostatin mRNA levels in the soleus muscles were significantly elevated in mice treated with PPE, and negatively related to grip strength, but not bone parameters, in mice treated with or without 2 U PPE in simple regression analyses. Grip strength positively related to bone parameters in mice treated with or without PPE. In conclusion, we showed that a PPE model of COPD in mice exerts dominant effects on bone rather than skeletal muscles. Increased myostatin expression in the soleus muscles of mice in the COPD state may negatively relate to a reduction in grip strength, but not bone loss. |
| first_indexed | 2024-03-09T00:19:20Z |
| format | Article |
| id | doaj.art-6f135a8ad3bf4be09de3912b2c0b06f0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-09T00:19:20Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6f135a8ad3bf4be09de3912b2c0b06f02023-12-12T05:36:02ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01186e028754110.1371/journal.pone.0287541Effects of elastase-induced emphysema on muscle and bone in mice.Daichi MatsumuraNaoyuki KawaoKatsumi OkumotoTakashi OhiraYuya MizukamiMasao AkagiHiroshi KajiChronic obstructive pulmonary disease (COPD) causes sarcopenia and osteoporosis. However, the mechanisms underlying muscle and bone loss as well as the interactions between muscle and bone in the COPD state remain unclear. Therefore, we herein investigated the effects of the COPD state on muscle and bone in mice intratracheally administered porcine pancreatic elastase (PPE). The intratracheal administration of PPE to mice significantly reduced trabecular bone mineral density (BMD), trabecular bone volume, trabecular number, cortical BMD and cortical area. It also significantly decreased grip strength, but did not affect muscle mass or the expression of myogenic differentiation-, protein degradation- or autophagy-related genes in the soleus and gastrocnemius muscles. Among the myokines examined, myostatin mRNA levels in the soleus muscles were significantly elevated in mice treated with PPE, and negatively related to grip strength, but not bone parameters, in mice treated with or without 2 U PPE in simple regression analyses. Grip strength positively related to bone parameters in mice treated with or without PPE. In conclusion, we showed that a PPE model of COPD in mice exerts dominant effects on bone rather than skeletal muscles. Increased myostatin expression in the soleus muscles of mice in the COPD state may negatively relate to a reduction in grip strength, but not bone loss.https://doi.org/10.1371/journal.pone.0287541 |
| spellingShingle | Daichi Matsumura Naoyuki Kawao Katsumi Okumoto Takashi Ohira Yuya Mizukami Masao Akagi Hiroshi Kaji Effects of elastase-induced emphysema on muscle and bone in mice. PLoS ONE |
| title | Effects of elastase-induced emphysema on muscle and bone in mice. |
| title_full | Effects of elastase-induced emphysema on muscle and bone in mice. |
| title_fullStr | Effects of elastase-induced emphysema on muscle and bone in mice. |
| title_full_unstemmed | Effects of elastase-induced emphysema on muscle and bone in mice. |
| title_short | Effects of elastase-induced emphysema on muscle and bone in mice. |
| title_sort | effects of elastase induced emphysema on muscle and bone in mice |
| url | https://doi.org/10.1371/journal.pone.0287541 |
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