Comparative transcriptomic analyses of glucosinolate metabolic genes during the formation of Chinese kale seeds
Abstract Background To understand the mechanism of glucosinolates (GSs) accumulation in the specific organs, combined analysis of physiological change and transcriptome sequencing were applied in the current study. Taking Chinese kale as material, seeds and silique walls were divided into different...
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| Format: | Article |
| Language: | English |
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BMC
2021-08-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-021-03168-2 |
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| author | Yijiao Zhao Zeyuan Chen Jiaxuan Chen Bingxing Chen Weiling Tang Xiaodong Chen Zhongxiong Lai Rongfang Guo |
| author_facet | Yijiao Zhao Zeyuan Chen Jiaxuan Chen Bingxing Chen Weiling Tang Xiaodong Chen Zhongxiong Lai Rongfang Guo |
| author_sort | Yijiao Zhao |
| collection | DOAJ |
| description | Abstract Background To understand the mechanism of glucosinolates (GSs) accumulation in the specific organs, combined analysis of physiological change and transcriptome sequencing were applied in the current study. Taking Chinese kale as material, seeds and silique walls were divided into different stages based on the development of the embryo in seeds and then subjected to GS analysis and transcriptome sequencing. Results The main GS in seeds of Chinese kale were glucoiberin and gluconapin and their content changed with the development of the seed. During the transition of the embryo from torpedo- to the early cotyledonary-embryo stage, the accumulation of GS in the seed was accompanied by the salient decline of GS in the corresponding silique wall. Thus, the seed and corresponding silique wall at these two stages were subjected to transcriptomic sequencing analysis. 135 genes related to GS metabolism were identified, of which 24 genes were transcription factors, 81 genes were related to biosynthetic pathway, 25 genes encoded catabolic enzymes, and 5 genes matched with transporters. The expression of GS biosynthetic genes was detected both in seeds and silique walls. The high expression of FMOGS-OX and AOP2, which is related to the production of gluconapin by side modification, was noted in seeds at both stages. Interestingly, the expression of GS biosynthetic genes was higher in the silique wall compared with that in the seed albeit lower content of GS existed in the silique wall than in the seed. Combined with the higher expression of transporter genes GTRs in silique walls than in seeds, it was proposed that the transportation of GS from the silique wall to the seed is an important source for seed GS accumulation. In addition, genes related to GS degradation expressed abundantly in the seed at the early cotyledonary-embryo stage indicating its potential role in balancing seed GS content. Conclusions Two stages including the torpedo-embryo and the early cotyledonary-embryo stage were identified as crucial in GS accumulation during seed development. Moreover, we confirmed the transportation of GS from the silique wall to the seed and proposed possible sidechain modification of GS biosynthesis may exist during seed formation. |
| first_indexed | 2024-12-16T08:59:59Z |
| format | Article |
| id | doaj.art-6f138344481641eba27a41ded71d3c1a |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-16T08:59:59Z |
| publishDate | 2021-08-01 |
| publisher | BMC |
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| series | BMC Plant Biology |
| spelling | doaj.art-6f138344481641eba27a41ded71d3c1a2022-12-21T22:37:11ZengBMCBMC Plant Biology1471-22292021-08-0121111510.1186/s12870-021-03168-2Comparative transcriptomic analyses of glucosinolate metabolic genes during the formation of Chinese kale seedsYijiao Zhao0Zeyuan Chen1Jiaxuan Chen2Bingxing Chen3Weiling Tang4Xiaodong Chen5Zhongxiong Lai6Rongfang Guo7College of Horticulture, Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry UniversityCollege of Horticulture, Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry UniversityJoint FAFU-Dalhousie Lab, College of Horticulture, Fujian Agriculture and Forestry UniversityJoint FAFU-Dalhousie Lab, College of Horticulture, Fujian Agriculture and Forestry UniversityCollege of Horticulture, Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry UniversityCollege of Horticulture, Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry UniversityCollege of Horticulture, Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry UniversityCollege of Horticulture, Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry UniversityAbstract Background To understand the mechanism of glucosinolates (GSs) accumulation in the specific organs, combined analysis of physiological change and transcriptome sequencing were applied in the current study. Taking Chinese kale as material, seeds and silique walls were divided into different stages based on the development of the embryo in seeds and then subjected to GS analysis and transcriptome sequencing. Results The main GS in seeds of Chinese kale were glucoiberin and gluconapin and their content changed with the development of the seed. During the transition of the embryo from torpedo- to the early cotyledonary-embryo stage, the accumulation of GS in the seed was accompanied by the salient decline of GS in the corresponding silique wall. Thus, the seed and corresponding silique wall at these two stages were subjected to transcriptomic sequencing analysis. 135 genes related to GS metabolism were identified, of which 24 genes were transcription factors, 81 genes were related to biosynthetic pathway, 25 genes encoded catabolic enzymes, and 5 genes matched with transporters. The expression of GS biosynthetic genes was detected both in seeds and silique walls. The high expression of FMOGS-OX and AOP2, which is related to the production of gluconapin by side modification, was noted in seeds at both stages. Interestingly, the expression of GS biosynthetic genes was higher in the silique wall compared with that in the seed albeit lower content of GS existed in the silique wall than in the seed. Combined with the higher expression of transporter genes GTRs in silique walls than in seeds, it was proposed that the transportation of GS from the silique wall to the seed is an important source for seed GS accumulation. In addition, genes related to GS degradation expressed abundantly in the seed at the early cotyledonary-embryo stage indicating its potential role in balancing seed GS content. Conclusions Two stages including the torpedo-embryo and the early cotyledonary-embryo stage were identified as crucial in GS accumulation during seed development. Moreover, we confirmed the transportation of GS from the silique wall to the seed and proposed possible sidechain modification of GS biosynthesis may exist during seed formation.https://doi.org/10.1186/s12870-021-03168-2GlucosinolatesSeedChinese kaleAOP2beta-glucosidasesGTR |
| spellingShingle | Yijiao Zhao Zeyuan Chen Jiaxuan Chen Bingxing Chen Weiling Tang Xiaodong Chen Zhongxiong Lai Rongfang Guo Comparative transcriptomic analyses of glucosinolate metabolic genes during the formation of Chinese kale seeds BMC Plant Biology Glucosinolates Seed Chinese kale AOP2 beta-glucosidases GTR |
| title | Comparative transcriptomic analyses of glucosinolate metabolic genes during the formation of Chinese kale seeds |
| title_full | Comparative transcriptomic analyses of glucosinolate metabolic genes during the formation of Chinese kale seeds |
| title_fullStr | Comparative transcriptomic analyses of glucosinolate metabolic genes during the formation of Chinese kale seeds |
| title_full_unstemmed | Comparative transcriptomic analyses of glucosinolate metabolic genes during the formation of Chinese kale seeds |
| title_short | Comparative transcriptomic analyses of glucosinolate metabolic genes during the formation of Chinese kale seeds |
| title_sort | comparative transcriptomic analyses of glucosinolate metabolic genes during the formation of chinese kale seeds |
| topic | Glucosinolates Seed Chinese kale AOP2 beta-glucosidases GTR |
| url | https://doi.org/10.1186/s12870-021-03168-2 |
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