Efficacy of orally administered montmorillonite in myoglobinuric acute renal failure model in male rats
Objective(s): Acute kidney injury can be associated with serious consequences and therefore early treatment is critical to decreasing mortality and morbidity rate. We evaluated the effect of montmorillonite, the clay with strong cation exchange capacity, on the AKI model in rats.Materials and Method...
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Mashhad University of Medical Sciences
2023-07-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_22240_edb4e71045d9cd15efd4472ce2f40fcf.pdf |
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| author | Seyed Ali Hosseini Azad Mohamad Moshiri Ali Roohbakhsh Abolfazl Shakeri Ashkan Fatemi Shandiz Leila Etemad |
| author_facet | Seyed Ali Hosseini Azad Mohamad Moshiri Ali Roohbakhsh Abolfazl Shakeri Ashkan Fatemi Shandiz Leila Etemad |
| author_sort | Seyed Ali Hosseini Azad |
| collection | DOAJ |
| description | Objective(s): Acute kidney injury can be associated with serious consequences and therefore early treatment is critical to decreasing mortality and morbidity rate. We evaluated the effect of montmorillonite, the clay with strong cation exchange capacity, on the AKI model in rats.Materials and Methods: Glycerol (50% solution, 10 ml/kg) was injected in the rat hind limbs to induce AKI. 24 hr after induction of acute kidney injury, the rats received oral doses of montmorillonite (0.5 g/kg or 1 g/kg), or sodium polystyrene sulfonate (1 g/kg) for three consecutive days. Results: Glycine induced acute kidney injury in rats with high levels of urea (336.60± 28.19 mg/dl), creatinine (4.10± 0.21 mg/dl), potassium (6.15 ± 0.28 mEq/L), and calcium (11.52 ± 0.19 mg/dl). Both doses of montmorillonite (0.5 and 1 g/kg) improved the serum urea (222.66± 10.02 and 170.20±8.06, P<0.05), creatinine (1.86±0.1, 2.05± 0.11, P<0.05), potassium (4.68 ± 0.4, 4.73 ± 0.34, P<0.001) and calcium (11.15 ± 0.17, 10.75 ± 0.25, P<0.01) levels. Treatment with montmorillonite especially at a high dose reduced the kidney pathological findings including, tubular necrosis, amorphous protein aggregation, and cell shedding into the distal and proximal tubule lumen. However, administration of SPS could not significantly decrease the severity of damages.Conclusion: According to the results of this study, as well as the physicochemical properties of montmorillonite, such as high ion exchange capacity and low side effects, montmorillonite can be a low-cost and effective treatment option to reduce and improve the complications of acute kidney injury. However, the efficacy of this compound in human and clinical studies needs to be investigated. |
| first_indexed | 2024-03-12T00:04:10Z |
| format | Article |
| id | doaj.art-6f157832292a47f694664300f315d01e |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-03-12T00:04:10Z |
| publishDate | 2023-07-01 |
| publisher | Mashhad University of Medical Sciences |
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| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-6f157832292a47f694664300f315d01e2023-09-17T06:58:08ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742023-07-0126775375910.22038/ijbms.2023.67985.1486622240Efficacy of orally administered montmorillonite in myoglobinuric acute renal failure model in male ratsSeyed Ali Hosseini Azad0Mohamad Moshiri1Ali Roohbakhsh2Abolfazl Shakeri3Ashkan Fatemi Shandiz4Leila Etemad5School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranSchool of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran 2 Medical Toxicology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranSchool of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranInternational UNESCO Center for Health-Related Basic Sciences and Human Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranObjective(s): Acute kidney injury can be associated with serious consequences and therefore early treatment is critical to decreasing mortality and morbidity rate. We evaluated the effect of montmorillonite, the clay with strong cation exchange capacity, on the AKI model in rats.Materials and Methods: Glycerol (50% solution, 10 ml/kg) was injected in the rat hind limbs to induce AKI. 24 hr after induction of acute kidney injury, the rats received oral doses of montmorillonite (0.5 g/kg or 1 g/kg), or sodium polystyrene sulfonate (1 g/kg) for three consecutive days. Results: Glycine induced acute kidney injury in rats with high levels of urea (336.60± 28.19 mg/dl), creatinine (4.10± 0.21 mg/dl), potassium (6.15 ± 0.28 mEq/L), and calcium (11.52 ± 0.19 mg/dl). Both doses of montmorillonite (0.5 and 1 g/kg) improved the serum urea (222.66± 10.02 and 170.20±8.06, P<0.05), creatinine (1.86±0.1, 2.05± 0.11, P<0.05), potassium (4.68 ± 0.4, 4.73 ± 0.34, P<0.001) and calcium (11.15 ± 0.17, 10.75 ± 0.25, P<0.01) levels. Treatment with montmorillonite especially at a high dose reduced the kidney pathological findings including, tubular necrosis, amorphous protein aggregation, and cell shedding into the distal and proximal tubule lumen. However, administration of SPS could not significantly decrease the severity of damages.Conclusion: According to the results of this study, as well as the physicochemical properties of montmorillonite, such as high ion exchange capacity and low side effects, montmorillonite can be a low-cost and effective treatment option to reduce and improve the complications of acute kidney injury. However, the efficacy of this compound in human and clinical studies needs to be investigated.https://ijbms.mums.ac.ir/article_22240_edb4e71045d9cd15efd4472ce2f40fcf.pdfacute renal failurebentoniteclaykidneymontmorilloniterhabdomyolysis |
| spellingShingle | Seyed Ali Hosseini Azad Mohamad Moshiri Ali Roohbakhsh Abolfazl Shakeri Ashkan Fatemi Shandiz Leila Etemad Efficacy of orally administered montmorillonite in myoglobinuric acute renal failure model in male rats Iranian Journal of Basic Medical Sciences acute renal failure bentonite clay kidney montmorillonite rhabdomyolysis |
| title | Efficacy of orally administered montmorillonite in myoglobinuric acute renal failure model in male rats |
| title_full | Efficacy of orally administered montmorillonite in myoglobinuric acute renal failure model in male rats |
| title_fullStr | Efficacy of orally administered montmorillonite in myoglobinuric acute renal failure model in male rats |
| title_full_unstemmed | Efficacy of orally administered montmorillonite in myoglobinuric acute renal failure model in male rats |
| title_short | Efficacy of orally administered montmorillonite in myoglobinuric acute renal failure model in male rats |
| title_sort | efficacy of orally administered montmorillonite in myoglobinuric acute renal failure model in male rats |
| topic | acute renal failure bentonite clay kidney montmorillonite rhabdomyolysis |
| url | https://ijbms.mums.ac.ir/article_22240_edb4e71045d9cd15efd4472ce2f40fcf.pdf |
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