Enamel matrix derivative (EMD) enhances the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs)
To investigate the EMD’s capacity in BMSCs osteogenic differentiation. In vivo and in vitro, BMSCs were treated with EMD, scanning electron microscopy, and Alizarin Red staining were used to detect the changes in the osteogenic ability of BMSCs, and the proliferation ability of BMSCs was evaluated b...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2021-01-01
|
| Series: | Bioengineered |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21655979.2021.1971504 |
| _version_ | 1798034059081809920 |
|---|---|
| author | Lu Cheng Ying Li Qian Xia MaoHua Meng ZhaoYang Ye ZhengLong Tang HongChao Feng Xin Chen HeLin Chen Xiao Zeng Yi Luo Qiang Dong |
| author_facet | Lu Cheng Ying Li Qian Xia MaoHua Meng ZhaoYang Ye ZhengLong Tang HongChao Feng Xin Chen HeLin Chen Xiao Zeng Yi Luo Qiang Dong |
| author_sort | Lu Cheng |
| collection | DOAJ |
| description | To investigate the EMD’s capacity in BMSCs osteogenic differentiation. In vivo and in vitro, BMSCs were treated with EMD, scanning electron microscopy, and Alizarin Red staining were used to detect the changes in the osteogenic ability of BMSCs, and the proliferation ability of BMSCs was evaluated by CCK8. In addition, by adding xav939, a typical inhibitor of Wnt/β-catenin signaling pathway, the regulatory function of Wnt/β-catenin signaling was clarified. The results showed that EMD promote cell proliferation and 25 μg/ml EMD had the most significant effect. Cells inducing osteogenesis for 2 and 3 even 4 weeks, the cell staining is deeper in EMD treated group than that of the control (P < 0.05) by alizarin Red staining, suggesting more mineralization of BMSCs. In vivo implanting the titanium plate wrapped with 25 μg/ml EMD treated-BMSC film into nude mice for 8 weeks, more nodules were formed on the surface of the titanium plate than that the control (P < 0.05). HE showed that there is a little blue-violet immature bone-like tissue block. Besides, the expression of RUNX Family Transcription Factor 2 (Runx2), Osterix, Osteocalcin (OCN), collagen I (COLI), alkaline phosphatase (ALP) and β-catenin were inhibited in xav939 group (P < 0.05); Inversely, all were activated in EMD group (P < 0.05). In conclusion, EMD promoted the proliferation and osteogenic differentiation of BMSCs. EMD’s function on BMSCs might be associated with the Wnt/β-catenin signaling pathway. |
| first_indexed | 2024-04-11T20:37:50Z |
| format | Article |
| id | doaj.art-6f183a61b16146d887f0259a5b02975f |
| institution | Directory Open Access Journal |
| issn | 2165-5979 2165-5987 |
| language | English |
| last_indexed | 2024-04-11T20:37:50Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Bioengineered |
| spelling | doaj.art-6f183a61b16146d887f0259a5b02975f2022-12-22T04:04:18ZengTaylor & Francis GroupBioengineered2165-59792165-59872021-01-011217033704510.1080/21655979.2021.19715041971504Enamel matrix derivative (EMD) enhances the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs)Lu Cheng0Ying Li1Qian Xia2MaoHua Meng3ZhaoYang Ye4ZhengLong Tang5HongChao Feng6Xin Chen7HeLin Chen8Xiao Zeng9Yi Luo10Qiang Dong11Guiyang Hospital of StomatologyStomatological hospital of Guizhou Medical UniversityStomatological hospital of Guizhou Medical UniversityGuizhou Medical Universitythe Affiliated Hospital of Guizhou Medical UniversityGuizhou Medical UniversityGuiyang Hospital of StomatologyGuizhou Medical UniversityStomatological hospital of Guizhou Medical UniversityStomatological hospital of Guizhou Medical UniversityGuiyang Hospital of StomatologyGuizhou Medical UniversityTo investigate the EMD’s capacity in BMSCs osteogenic differentiation. In vivo and in vitro, BMSCs were treated with EMD, scanning electron microscopy, and Alizarin Red staining were used to detect the changes in the osteogenic ability of BMSCs, and the proliferation ability of BMSCs was evaluated by CCK8. In addition, by adding xav939, a typical inhibitor of Wnt/β-catenin signaling pathway, the regulatory function of Wnt/β-catenin signaling was clarified. The results showed that EMD promote cell proliferation and 25 μg/ml EMD had the most significant effect. Cells inducing osteogenesis for 2 and 3 even 4 weeks, the cell staining is deeper in EMD treated group than that of the control (P < 0.05) by alizarin Red staining, suggesting more mineralization of BMSCs. In vivo implanting the titanium plate wrapped with 25 μg/ml EMD treated-BMSC film into nude mice for 8 weeks, more nodules were formed on the surface of the titanium plate than that the control (P < 0.05). HE showed that there is a little blue-violet immature bone-like tissue block. Besides, the expression of RUNX Family Transcription Factor 2 (Runx2), Osterix, Osteocalcin (OCN), collagen I (COLI), alkaline phosphatase (ALP) and β-catenin were inhibited in xav939 group (P < 0.05); Inversely, all were activated in EMD group (P < 0.05). In conclusion, EMD promoted the proliferation and osteogenic differentiation of BMSCs. EMD’s function on BMSCs might be associated with the Wnt/β-catenin signaling pathway.http://dx.doi.org/10.1080/21655979.2021.1971504enamel matrix derivativebone marrow mesenchymal stem cellswnt/β-catenin signaling |
| spellingShingle | Lu Cheng Ying Li Qian Xia MaoHua Meng ZhaoYang Ye ZhengLong Tang HongChao Feng Xin Chen HeLin Chen Xiao Zeng Yi Luo Qiang Dong Enamel matrix derivative (EMD) enhances the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) Bioengineered enamel matrix derivative bone marrow mesenchymal stem cells wnt/β-catenin signaling |
| title | Enamel matrix derivative (EMD) enhances the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) |
| title_full | Enamel matrix derivative (EMD) enhances the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) |
| title_fullStr | Enamel matrix derivative (EMD) enhances the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) |
| title_full_unstemmed | Enamel matrix derivative (EMD) enhances the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) |
| title_short | Enamel matrix derivative (EMD) enhances the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) |
| title_sort | enamel matrix derivative emd enhances the osteogenic differentiation of bone marrow mesenchymal stem cells bmscs |
| topic | enamel matrix derivative bone marrow mesenchymal stem cells wnt/β-catenin signaling |
| url | http://dx.doi.org/10.1080/21655979.2021.1971504 |
| work_keys_str_mv | AT lucheng enamelmatrixderivativeemdenhancestheosteogenicdifferentiationofbonemarrowmesenchymalstemcellsbmscs AT yingli enamelmatrixderivativeemdenhancestheosteogenicdifferentiationofbonemarrowmesenchymalstemcellsbmscs AT qianxia enamelmatrixderivativeemdenhancestheosteogenicdifferentiationofbonemarrowmesenchymalstemcellsbmscs AT maohuameng enamelmatrixderivativeemdenhancestheosteogenicdifferentiationofbonemarrowmesenchymalstemcellsbmscs AT zhaoyangye enamelmatrixderivativeemdenhancestheosteogenicdifferentiationofbonemarrowmesenchymalstemcellsbmscs AT zhenglongtang enamelmatrixderivativeemdenhancestheosteogenicdifferentiationofbonemarrowmesenchymalstemcellsbmscs AT hongchaofeng enamelmatrixderivativeemdenhancestheosteogenicdifferentiationofbonemarrowmesenchymalstemcellsbmscs AT xinchen enamelmatrixderivativeemdenhancestheosteogenicdifferentiationofbonemarrowmesenchymalstemcellsbmscs AT helinchen enamelmatrixderivativeemdenhancestheosteogenicdifferentiationofbonemarrowmesenchymalstemcellsbmscs AT xiaozeng enamelmatrixderivativeemdenhancestheosteogenicdifferentiationofbonemarrowmesenchymalstemcellsbmscs AT yiluo enamelmatrixderivativeemdenhancestheosteogenicdifferentiationofbonemarrowmesenchymalstemcellsbmscs AT qiangdong enamelmatrixderivativeemdenhancestheosteogenicdifferentiationofbonemarrowmesenchymalstemcellsbmscs |