Novel biomarkers of inflammation-associated immunity in cervical cancer
BackgroundCervical cancer (CC) is a highly malignant gynecological cancer with a direct causal link to inflammation, primarily resulting from persistent high-risk human papillomavirus (HPV) infection. Given the challenges in early detection and mid to late-stage treatment, our research aims to ident...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2024.1351736/full |
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| author | Weihong Zhao Qi Li Qi Li Songquan Wen Songquan Wen Yaqin Li Ying Bai Ying Bai Zhiyu Tian Zhiyu Tian |
| author_facet | Weihong Zhao Qi Li Qi Li Songquan Wen Songquan Wen Yaqin Li Ying Bai Ying Bai Zhiyu Tian Zhiyu Tian |
| author_sort | Weihong Zhao |
| collection | DOAJ |
| description | BackgroundCervical cancer (CC) is a highly malignant gynecological cancer with a direct causal link to inflammation, primarily resulting from persistent high-risk human papillomavirus (HPV) infection. Given the challenges in early detection and mid to late-stage treatment, our research aims to identify inflammation-associated immune biomarkers in CC.MethodsUsing a bioinformatics approach combined with experimental validation, we integrated two CC datasets (GSE39001 and GSE63514) in the Gene Expression Omnibus (GEO) to eliminate batch effects. Immune-related inflammation differentially expressed genes (DGEs) were obtained by R language identification.ResultsThis analysis identified 37 inflammation-related DEGs. Subsequently, we discussed the different levels of immune infiltration between CC cases and controls. Weighted gene co-expression network analysis (WGCNA) identified seven immune infiltration-related modules in CC. We identified 15 immune DEGs associated with inflammation at the intersection of these findings. In addition, we constructed a protein interaction network using the String database and screened five hub genes using "CytoHubba": CXC chemokine ligand 8 (CXCL8), CXC chemokine ligand 10 (CXCL10), CX3C chemokine receptor 1 (CX3CR1), Fc gamma receptors 3B (FCGR3B), and SELL. The expression of these five genes in CC was determined by PCR experiments. In addition, we assessed their diagnostic value and further analyzed the association of immune cells with them.ConclusionsFive inflammation- and immune-related genes were identified, aiming to provide new directions for early diagnosis and mid to late-stage treatment of CC from multiple perspectives. |
| first_indexed | 2024-04-25T00:37:38Z |
| format | Article |
| id | doaj.art-6f1a5dcd4d0546549924e58e8aaef0d5 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-25T00:37:38Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-6f1a5dcd4d0546549924e58e8aaef0d52024-03-12T13:25:32ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2024-03-011410.3389/fonc.2024.13517361351736Novel biomarkers of inflammation-associated immunity in cervical cancerWeihong Zhao0Qi Li1Qi Li2Songquan Wen3Songquan Wen4Yaqin Li5Ying Bai6Ying Bai7Zhiyu Tian8Zhiyu Tian9Department of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaThe Second Clinical Medical College, Shanxi Medical University, Taiyuan, ChinaDepartment of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaThe Second Clinical Medical College, Shanxi Medical University, Taiyuan, ChinaDepartment of Obstetrics and Gynecology, Peking University People’s Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaThe Second Clinical Medical College, Shanxi Medical University, Taiyuan, ChinaThe Second Clinical Medical College, Shanxi Medical University, Taiyuan, ChinaDepartment of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan, Shanxi, ChinaBackgroundCervical cancer (CC) is a highly malignant gynecological cancer with a direct causal link to inflammation, primarily resulting from persistent high-risk human papillomavirus (HPV) infection. Given the challenges in early detection and mid to late-stage treatment, our research aims to identify inflammation-associated immune biomarkers in CC.MethodsUsing a bioinformatics approach combined with experimental validation, we integrated two CC datasets (GSE39001 and GSE63514) in the Gene Expression Omnibus (GEO) to eliminate batch effects. Immune-related inflammation differentially expressed genes (DGEs) were obtained by R language identification.ResultsThis analysis identified 37 inflammation-related DEGs. Subsequently, we discussed the different levels of immune infiltration between CC cases and controls. Weighted gene co-expression network analysis (WGCNA) identified seven immune infiltration-related modules in CC. We identified 15 immune DEGs associated with inflammation at the intersection of these findings. In addition, we constructed a protein interaction network using the String database and screened five hub genes using "CytoHubba": CXC chemokine ligand 8 (CXCL8), CXC chemokine ligand 10 (CXCL10), CX3C chemokine receptor 1 (CX3CR1), Fc gamma receptors 3B (FCGR3B), and SELL. The expression of these five genes in CC was determined by PCR experiments. In addition, we assessed their diagnostic value and further analyzed the association of immune cells with them.ConclusionsFive inflammation- and immune-related genes were identified, aiming to provide new directions for early diagnosis and mid to late-stage treatment of CC from multiple perspectives.https://www.frontiersin.org/articles/10.3389/fonc.2024.1351736/fullcervical cancerimmune infiltrationdifferentially expressed inflammation-related genesinflammation-associated immune biomarkersCIBERSORT |
| spellingShingle | Weihong Zhao Qi Li Qi Li Songquan Wen Songquan Wen Yaqin Li Ying Bai Ying Bai Zhiyu Tian Zhiyu Tian Novel biomarkers of inflammation-associated immunity in cervical cancer Frontiers in Oncology cervical cancer immune infiltration differentially expressed inflammation-related genes inflammation-associated immune biomarkers CIBERSORT |
| title | Novel biomarkers of inflammation-associated immunity in cervical cancer |
| title_full | Novel biomarkers of inflammation-associated immunity in cervical cancer |
| title_fullStr | Novel biomarkers of inflammation-associated immunity in cervical cancer |
| title_full_unstemmed | Novel biomarkers of inflammation-associated immunity in cervical cancer |
| title_short | Novel biomarkers of inflammation-associated immunity in cervical cancer |
| title_sort | novel biomarkers of inflammation associated immunity in cervical cancer |
| topic | cervical cancer immune infiltration differentially expressed inflammation-related genes inflammation-associated immune biomarkers CIBERSORT |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2024.1351736/full |
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