Antibody Fragments as Probe in Biosensor Development
Today’s proteomic analyses are generating increasing numbers of biomarkers, making it essential to possess highly specific probes able to recognize those targets. Antibodies are considered to be the first choice as molecular recognition units due to their target specificity and affinity, which...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2008-08-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/8/8/4669/ |
| _version_ | 1811187265781628928 |
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| author | Serge Muyldermans Kristien Bonroy Lieven Huang Dirk Saerens |
| author_facet | Serge Muyldermans Kristien Bonroy Lieven Huang Dirk Saerens |
| author_sort | Serge Muyldermans |
| collection | DOAJ |
| description | Today’s proteomic analyses are generating increasing numbers of biomarkers, making it essential to possess highly specific probes able to recognize those targets. Antibodies are considered to be the first choice as molecular recognition units due to their target specificity and affinity, which make them excellent probes in biosensor development. However several problems such as difficult directional immobilization, unstable behavior, loss of specificity and steric hindrance, may arise from using these large molecules. Luckily, protein engineering techniques offer designed antibody formats suitable for biomarker analysis. Minimization strategies of antibodies into Fab fragments, scFv or even single-domain antibody fragments like VH, VL or VHHs are reviewed. Not only the size of the probe but also other issues like choice of immobilization tag, type of solid support and probe stability are of critical importance in assay development for biosensing. In this respect, multiple approaches to specifically orient and couple antibody fragments in a generic one-step procedure directly on a biosensor substrate are discussed. |
| first_indexed | 2024-04-11T13:59:22Z |
| format | Article |
| id | doaj.art-6f1b5d9f0d4848d8a08b6f0c604768d9 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T13:59:22Z |
| publishDate | 2008-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-6f1b5d9f0d4848d8a08b6f0c604768d92022-12-22T04:20:10ZengMDPI AGSensors1424-82202008-08-018846694686Antibody Fragments as Probe in Biosensor DevelopmentSerge MuyldermansKristien BonroyLieven HuangDirk SaerensToday’s proteomic analyses are generating increasing numbers of biomarkers, making it essential to possess highly specific probes able to recognize those targets. Antibodies are considered to be the first choice as molecular recognition units due to their target specificity and affinity, which make them excellent probes in biosensor development. However several problems such as difficult directional immobilization, unstable behavior, loss of specificity and steric hindrance, may arise from using these large molecules. Luckily, protein engineering techniques offer designed antibody formats suitable for biomarker analysis. Minimization strategies of antibodies into Fab fragments, scFv or even single-domain antibody fragments like VH, VL or VHHs are reviewed. Not only the size of the probe but also other issues like choice of immobilization tag, type of solid support and probe stability are of critical importance in assay development for biosensing. In this respect, multiple approaches to specifically orient and couple antibody fragments in a generic one-step procedure directly on a biosensor substrate are discussed.http://www.mdpi.com/1424-8220/8/8/4669/display technologyaffinitystabilityimmobilizationimmunoassay. |
| spellingShingle | Serge Muyldermans Kristien Bonroy Lieven Huang Dirk Saerens Antibody Fragments as Probe in Biosensor Development Sensors display technology affinity stability immobilization immunoassay. |
| title | Antibody Fragments as Probe in Biosensor Development |
| title_full | Antibody Fragments as Probe in Biosensor Development |
| title_fullStr | Antibody Fragments as Probe in Biosensor Development |
| title_full_unstemmed | Antibody Fragments as Probe in Biosensor Development |
| title_short | Antibody Fragments as Probe in Biosensor Development |
| title_sort | antibody fragments as probe in biosensor development |
| topic | display technology affinity stability immobilization immunoassay. |
| url | http://www.mdpi.com/1424-8220/8/8/4669/ |
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