Ethanol Production from Non-Food Tubers of Iles-iles (Amorphophallus campanulatus) by Using Separated Hydrolysis and Fermentation
The decrease in production and the raise in needs have led to the rise in oil prices. This work investigated the possibility of Iles-iles (Amorphophallus campanulatus) tuber flour, which is rich in carbohydrate con-tent, as a raw material to produce bioethanol. To obtain the maximum ethanol concentr...
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| Format: | Article |
| Language: | English |
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Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS)
2014-08-01
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| Series: | Bulletin of Chemical Reaction Engineering & Catalysis |
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| Online Access: | https://journal.bcrec.id/index.php/bcrec/article/view/19450 |
| _version_ | 1827810256720232448 |
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| author | Kusmiyati Kusmiyati |
| author_facet | Kusmiyati Kusmiyati |
| author_sort | Kusmiyati Kusmiyati |
| collection | DOAJ |
| description | The decrease in production and the raise in needs have led to the rise in oil prices. This work investigated the possibility of Iles-iles (Amorphophallus campanulatus) tuber flour, which is rich in carbohydrate con-tent, as a raw material to produce bioethanol. To obtain the maximum ethanol concentration, several parameters had been studied, such as: the concentration of α-amylase and β-amylase in liquefaction and sac-charification processes, respectively, the type of S. cerevisiae enzyme (pure, dry, wet and instant) and weight of Diammonium phosphate (DAP) as a nutrient for S. cerevisiae in fermentation. The result shows that the highest reducing sugar content (12.5%) was achieved when 3.2 ml α-amylase/kg flour and 6.4 ml β-amylase/kg flour were used during liquefaction and saccharification processes. Since the concentration of α- and β-amylase increased, the reducing sugar obtained also increased. The higher sugar content resulted the higher the ethanol concentration in the fermentation broth. Furthermore, the highest concentration of ethanol (9 %v/v) was obtained at 72 h fermentation using the dry S. cerevisiae, at 3.2 ml and 6.4 ml /kg flour of α-amylase and β-amylase enzymes, respectively. From the study of the effect of S. cerevisiae type, it was shown that dry S. cereviseae produced the highest ethanol concentration 10.2% (v/v) at 72 h fermentation. The DAP was used as a nitrogen supply required by S. cerevisiae to growth and as a results can increase the ethanol concentration. The addition of DAP in the fermentation proved that 8.45% (v/v) of ethanol was obtained. This result shows that the proposed tuber flour has the potential a raw material for bioethanol production. © 2014 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0) |
| first_indexed | 2024-03-11T22:49:44Z |
| format | Article |
| id | doaj.art-6f249e1eafda40058475eaa5099c9f02 |
| institution | Directory Open Access Journal |
| issn | 1978-2993 |
| language | English |
| last_indexed | 2024-03-11T22:49:44Z |
| publishDate | 2014-08-01 |
| publisher | Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS) |
| record_format | Article |
| series | Bulletin of Chemical Reaction Engineering & Catalysis |
| spelling | doaj.art-6f249e1eafda40058475eaa5099c9f022023-09-22T03:55:47ZengMasyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS)Bulletin of Chemical Reaction Engineering & Catalysis1978-29932014-08-0192939910.9767/bcrec.9.2.6014.93-998145Ethanol Production from Non-Food Tubers of Iles-iles (Amorphophallus campanulatus) by Using Separated Hydrolysis and FermentationKusmiyati Kusmiyati0Renewable Energy Research Centre, Department of Chemical Engineering, Faculty of Engineering, Muhammadiyah University of Surakarta, Jl. A. Yani Tromol Pos 1, Pabelan, Kartasura 57102, Surakarta, IndonesiaThe decrease in production and the raise in needs have led to the rise in oil prices. This work investigated the possibility of Iles-iles (Amorphophallus campanulatus) tuber flour, which is rich in carbohydrate con-tent, as a raw material to produce bioethanol. To obtain the maximum ethanol concentration, several parameters had been studied, such as: the concentration of α-amylase and β-amylase in liquefaction and sac-charification processes, respectively, the type of S. cerevisiae enzyme (pure, dry, wet and instant) and weight of Diammonium phosphate (DAP) as a nutrient for S. cerevisiae in fermentation. The result shows that the highest reducing sugar content (12.5%) was achieved when 3.2 ml α-amylase/kg flour and 6.4 ml β-amylase/kg flour were used during liquefaction and saccharification processes. Since the concentration of α- and β-amylase increased, the reducing sugar obtained also increased. The higher sugar content resulted the higher the ethanol concentration in the fermentation broth. Furthermore, the highest concentration of ethanol (9 %v/v) was obtained at 72 h fermentation using the dry S. cerevisiae, at 3.2 ml and 6.4 ml /kg flour of α-amylase and β-amylase enzymes, respectively. From the study of the effect of S. cerevisiae type, it was shown that dry S. cereviseae produced the highest ethanol concentration 10.2% (v/v) at 72 h fermentation. The DAP was used as a nitrogen supply required by S. cerevisiae to growth and as a results can increase the ethanol concentration. The addition of DAP in the fermentation proved that 8.45% (v/v) of ethanol was obtained. This result shows that the proposed tuber flour has the potential a raw material for bioethanol production. © 2014 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0)https://journal.bcrec.id/index.php/bcrec/article/view/19450biofuelbioethanoiles-iless. cerevisiaeα amylaseβ amylase |
| spellingShingle | Kusmiyati Kusmiyati Ethanol Production from Non-Food Tubers of Iles-iles (Amorphophallus campanulatus) by Using Separated Hydrolysis and Fermentation Bulletin of Chemical Reaction Engineering & Catalysis biofuel bioethano iles-iles s. cerevisiae α amylase β amylase |
| title | Ethanol Production from Non-Food Tubers of Iles-iles (Amorphophallus campanulatus) by Using Separated Hydrolysis and Fermentation |
| title_full | Ethanol Production from Non-Food Tubers of Iles-iles (Amorphophallus campanulatus) by Using Separated Hydrolysis and Fermentation |
| title_fullStr | Ethanol Production from Non-Food Tubers of Iles-iles (Amorphophallus campanulatus) by Using Separated Hydrolysis and Fermentation |
| title_full_unstemmed | Ethanol Production from Non-Food Tubers of Iles-iles (Amorphophallus campanulatus) by Using Separated Hydrolysis and Fermentation |
| title_short | Ethanol Production from Non-Food Tubers of Iles-iles (Amorphophallus campanulatus) by Using Separated Hydrolysis and Fermentation |
| title_sort | ethanol production from non food tubers of iles iles amorphophallus campanulatus by using separated hydrolysis and fermentation |
| topic | biofuel bioethano iles-iles s. cerevisiae α amylase β amylase |
| url | https://journal.bcrec.id/index.php/bcrec/article/view/19450 |
| work_keys_str_mv | AT kusmiyatikusmiyati ethanolproductionfromnonfoodtubersofilesilesamorphophalluscampanulatusbyusingseparatedhydrolysisandfermentation |