NF-KB P65-subunit activity and T-helpers 1 / T-helpers 2 ratio in pregnant women with placental disorders and premature labor
Objectives: to study the levels of the total, phosphorylated p65-subunit of the nuclear factor NF-kB, activity of p65 and the relation with the level and ratio of T-helpers type I and II in pregnant women with placental dysfunction and different clinical types of the course of preterm labor (with pr...
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| Format: | Article |
| Language: | English |
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Publishing House TRILIST
2023-03-01
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| Series: | Репродуктивная эндокринология |
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| Online Access: | http://reproduct-endo.com/article/view/276437 |
| _version_ | 1797647688469053440 |
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| author | I.B. Ventskivska V.I. Kupchik |
| author_facet | I.B. Ventskivska V.I. Kupchik |
| author_sort | I.B. Ventskivska |
| collection | DOAJ |
| description | Objectives: to study the levels of the total, phosphorylated p65-subunit of the nuclear factor NF-kB, activity of p65 and the relation with the level and ratio of T-helpers type I and II in pregnant women with placental dysfunction and different clinical types of the course of preterm labor (with preterm premature rupture of membranes (pPROM) and without it).
Materials and methods. The case-control study included 60 pregnant women: 40 women with placental disorders and spontaneous premature labor in the period of 24–34 weeks (group I – 20 women with premature labor and timely discharge of amniotic fluid, group II – 20 women with pPROM) and 20 women of the control group (CG) with normal timely delivery in the head position of a fetus without complications.
The value of the total NF-kB p65 subunit and its phosphorylated fraction was determined in all women using ELISA in placenta lysates. On this basis the p65 subunit activity was calculated; number of T-helper I (Th1) and T-helper II (Th2) was determined using flow cytometry in a whole blood sample, with afterward calculation of the Th1/Th2 ratio.
Results. Elevated levels of total p65 and its phosphorylated fraction were found in women with placental dysfunction (p < 0.01 in groups I and II compared with CG), as well as the activity p65 (p < 0.01 in group I, p = 0.04 in group II compared with CG). The difference in the Th1 value and the Th1/Th2 ratio was significantly higher in both groups (p < 0.01 in group I, p = 0.03 in group II for Th1; p < 0.01 in both groups for Th1/Th2), the number of Th2 differed significantly only in group I (p < 0.01 compared with CG). A strong positive correlation between p65 activity and Th1/Th2 was also established (r = 0.8).
Conclusions. Obtained data indicates the increased NF-kB p65-subunit activity in women with placental disorders and spontaneous premature labor without pPROM, which is impact on the increase of the Th1/Th2 ratio due to the Th1 increase. This mechanism might be considered to be the leading cause of the premature birth in this group of pregnant women. However, for women with the preterm labor activity with pPROM, the difference with GC has a lower level of significance, which may indicate the existence of another leading mechanism for the initiation of premature labor in this group. |
| first_indexed | 2024-03-11T15:21:07Z |
| format | Article |
| id | doaj.art-6f27fa8ea42e4eec8957473a756256ca |
| institution | Directory Open Access Journal |
| issn | 2309-4117 2411-1295 |
| language | English |
| last_indexed | 2024-03-11T15:21:07Z |
| publishDate | 2023-03-01 |
| publisher | Publishing House TRILIST |
| record_format | Article |
| series | Репродуктивная эндокринология |
| spelling | doaj.art-6f27fa8ea42e4eec8957473a756256ca2023-10-28T14:18:14ZengPublishing House TRILISTРепродуктивная эндокринология2309-41172411-12952023-03-0167798310.18370/2309-4117.2023.67.79-83314603NF-KB P65-subunit activity and T-helpers 1 / T-helpers 2 ratio in pregnant women with placental disorders and premature laborI.B. Ventskivska0https://orcid.org/0000-0002-7685-5289V.I. Kupchik1https://orcid.org/0000-0002-4394-260XO.O. Bogomolets National Medical University, Kyiv, UkraineO.O. Bogomolets National Medical University, Kyiv, UkraineObjectives: to study the levels of the total, phosphorylated p65-subunit of the nuclear factor NF-kB, activity of p65 and the relation with the level and ratio of T-helpers type I and II in pregnant women with placental dysfunction and different clinical types of the course of preterm labor (with preterm premature rupture of membranes (pPROM) and without it). Materials and methods. The case-control study included 60 pregnant women: 40 women with placental disorders and spontaneous premature labor in the period of 24–34 weeks (group I – 20 women with premature labor and timely discharge of amniotic fluid, group II – 20 women with pPROM) and 20 women of the control group (CG) with normal timely delivery in the head position of a fetus without complications. The value of the total NF-kB p65 subunit and its phosphorylated fraction was determined in all women using ELISA in placenta lysates. On this basis the p65 subunit activity was calculated; number of T-helper I (Th1) and T-helper II (Th2) was determined using flow cytometry in a whole blood sample, with afterward calculation of the Th1/Th2 ratio. Results. Elevated levels of total p65 and its phosphorylated fraction were found in women with placental dysfunction (p < 0.01 in groups I and II compared with CG), as well as the activity p65 (p < 0.01 in group I, p = 0.04 in group II compared with CG). The difference in the Th1 value and the Th1/Th2 ratio was significantly higher in both groups (p < 0.01 in group I, p = 0.03 in group II for Th1; p < 0.01 in both groups for Th1/Th2), the number of Th2 differed significantly only in group I (p < 0.01 compared with CG). A strong positive correlation between p65 activity and Th1/Th2 was also established (r = 0.8). Conclusions. Obtained data indicates the increased NF-kB p65-subunit activity in women with placental disorders and spontaneous premature labor without pPROM, which is impact on the increase of the Th1/Th2 ratio due to the Th1 increase. This mechanism might be considered to be the leading cause of the premature birth in this group of pregnant women. However, for women with the preterm labor activity with pPROM, the difference with GC has a lower level of significance, which may indicate the existence of another leading mechanism for the initiation of premature labor in this group.http://reproduct-endo.com/article/view/276437nf-kb p65-subunitt-helpers type 1t-helpers type 2premature birthplacental dysfunctionpreterm premature rupture of membranes |
| spellingShingle | I.B. Ventskivska V.I. Kupchik NF-KB P65-subunit activity and T-helpers 1 / T-helpers 2 ratio in pregnant women with placental disorders and premature labor Репродуктивная эндокринология nf-kb p65-subunit t-helpers type 1 t-helpers type 2 premature birth placental dysfunction preterm premature rupture of membranes |
| title | NF-KB P65-subunit activity and T-helpers 1 / T-helpers 2 ratio in pregnant women with placental disorders and premature labor |
| title_full | NF-KB P65-subunit activity and T-helpers 1 / T-helpers 2 ratio in pregnant women with placental disorders and premature labor |
| title_fullStr | NF-KB P65-subunit activity and T-helpers 1 / T-helpers 2 ratio in pregnant women with placental disorders and premature labor |
| title_full_unstemmed | NF-KB P65-subunit activity and T-helpers 1 / T-helpers 2 ratio in pregnant women with placental disorders and premature labor |
| title_short | NF-KB P65-subunit activity and T-helpers 1 / T-helpers 2 ratio in pregnant women with placental disorders and premature labor |
| title_sort | nf kb p65 subunit activity and t helpers 1 t helpers 2 ratio in pregnant women with placental disorders and premature labor |
| topic | nf-kb p65-subunit t-helpers type 1 t-helpers type 2 premature birth placental dysfunction preterm premature rupture of membranes |
| url | http://reproduct-endo.com/article/view/276437 |
| work_keys_str_mv | AT ibventskivska nfkbp65subunitactivityandthelpers1thelpers2ratioinpregnantwomenwithplacentaldisordersandprematurelabor AT vikupchik nfkbp65subunitactivityandthelpers1thelpers2ratioinpregnantwomenwithplacentaldisordersandprematurelabor |