Therapeutic and immunoregulatory effects of water-soluble alkaloids E2-a from Sophora moorcroftiana seeds as a novel potential agent against echinococcosis in experimentally protoscolex-infected mice
Abstract Novel compounds and more efficient treatment options are urgently needed for the treatment of cystic echinococcosis (CE), which is caused by Echinococcus granulosus. The decoction of Sophora moorcroftiana (Fabaceae) has been used to treat parasitosis for years in traditional Tibetan medicin...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-10-01
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| Series: | Veterinary Research |
| Online Access: | http://link.springer.com/article/10.1186/s13567-018-0596-9 |
| _version_ | 1819127659871862784 |
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| author | Yanping Luo Guochao Zhang Xun Liu Miaomiao Yuan Qi Gao Haijun Gao Lixin Ke Xinxing Zhang Yanbin Shi Xingming Ma Lifeng Zhang Kaizhong Dong |
| author_facet | Yanping Luo Guochao Zhang Xun Liu Miaomiao Yuan Qi Gao Haijun Gao Lixin Ke Xinxing Zhang Yanbin Shi Xingming Ma Lifeng Zhang Kaizhong Dong |
| author_sort | Yanping Luo |
| collection | DOAJ |
| description | Abstract Novel compounds and more efficient treatment options are urgently needed for the treatment of cystic echinococcosis (CE), which is caused by Echinococcus granulosus. The decoction of Sophora moorcroftiana (Fabaceae) has been used to treat parasitosis for years in traditional Tibetan medicine. The aim of this study was to screen insecticidal water-soluble alkaloids from S. moorcroftiana seeds and evaluate the therapeutic effects against CE and the immune response induced by the alkaloidal fraction. Low polarity compounds (E2-a) were isolated from water-soluble alkaloid (E2) and matrine and sophocarpine were identified as major components. The E2-a fraction was more effective against protoscoleces than other constituents from S. moorcroftiana. After 20 weeks of secondary infection with protoscoleces, mice were orally treated with E2-a (100 mg/kg/day) for 6 weeks to evaluate therapeutic and immunoregulatory activities. Compared with the untreated group, E2-a treatment induced a significant reduction in cyst weight (mean 2.93 g) (p < 0.05) and an impaired ultrastructural modification of the cyst. Interestingly, the application of E2-a resulted in a significant increased frequency of CD3+CD4+ T-cell subsets and decreased frequency of CD3+PD-1+ T-cell subsets, compared with protoscolece-infected mice without treatment. The E2-a fraction of S. moorcroftiana can inhibit the cyst development of CE and boost the specific immune response by reducing the expression of PD-1 and accelerate the cytokine secretion of antigen-specific T-cells. All data suggest the E2-a fraction from S. moorcroftiana seeds may be used as a new potential therapeutic option against E. granulosus infection. |
| first_indexed | 2024-12-22T08:15:27Z |
| format | Article |
| id | doaj.art-6f29e615b9124bdc82018bb0d2451784 |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-12-22T08:15:27Z |
| publishDate | 2018-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-6f29e615b9124bdc82018bb0d24517842022-12-21T18:32:55ZengBMCVeterinary Research1297-97162018-10-0149111310.1186/s13567-018-0596-9Therapeutic and immunoregulatory effects of water-soluble alkaloids E2-a from Sophora moorcroftiana seeds as a novel potential agent against echinococcosis in experimentally protoscolex-infected miceYanping Luo0Guochao Zhang1Xun Liu2Miaomiao Yuan3Qi Gao4Haijun Gao5Lixin Ke6Xinxing Zhang7Yanbin Shi8Xingming Ma9Lifeng Zhang10Kaizhong Dong11Department of Immunology, School of Basic Medical Sciences, Lanzhou UniversityDepartment of Immunology, School of Basic Medical Sciences, Lanzhou UniversityInstitute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou UniversityCancer Research Institute, School of Basic Medical Sciences, Southern Medical UniversityDepartment of Immunology, School of Basic Medical Sciences, Lanzhou UniversityInstitute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou UniversityDepartment of Immunology, School of Basic Medical Sciences, Lanzhou UniversityDepartment of Immunology, School of Basic Medical Sciences, Lanzhou UniversitySchool of Pharmacy, Lanzhou UniversityDepartment of Immunology, School of Basic Medical Sciences, Lanzhou UniversityDepartment of Immunology, School of Basic Medical Sciences, Lanzhou UniversityDepartment of Microbiology, Medical College, Northwest University for NationalitiesAbstract Novel compounds and more efficient treatment options are urgently needed for the treatment of cystic echinococcosis (CE), which is caused by Echinococcus granulosus. The decoction of Sophora moorcroftiana (Fabaceae) has been used to treat parasitosis for years in traditional Tibetan medicine. The aim of this study was to screen insecticidal water-soluble alkaloids from S. moorcroftiana seeds and evaluate the therapeutic effects against CE and the immune response induced by the alkaloidal fraction. Low polarity compounds (E2-a) were isolated from water-soluble alkaloid (E2) and matrine and sophocarpine were identified as major components. The E2-a fraction was more effective against protoscoleces than other constituents from S. moorcroftiana. After 20 weeks of secondary infection with protoscoleces, mice were orally treated with E2-a (100 mg/kg/day) for 6 weeks to evaluate therapeutic and immunoregulatory activities. Compared with the untreated group, E2-a treatment induced a significant reduction in cyst weight (mean 2.93 g) (p < 0.05) and an impaired ultrastructural modification of the cyst. Interestingly, the application of E2-a resulted in a significant increased frequency of CD3+CD4+ T-cell subsets and decreased frequency of CD3+PD-1+ T-cell subsets, compared with protoscolece-infected mice without treatment. The E2-a fraction of S. moorcroftiana can inhibit the cyst development of CE and boost the specific immune response by reducing the expression of PD-1 and accelerate the cytokine secretion of antigen-specific T-cells. All data suggest the E2-a fraction from S. moorcroftiana seeds may be used as a new potential therapeutic option against E. granulosus infection.http://link.springer.com/article/10.1186/s13567-018-0596-9 |
| spellingShingle | Yanping Luo Guochao Zhang Xun Liu Miaomiao Yuan Qi Gao Haijun Gao Lixin Ke Xinxing Zhang Yanbin Shi Xingming Ma Lifeng Zhang Kaizhong Dong Therapeutic and immunoregulatory effects of water-soluble alkaloids E2-a from Sophora moorcroftiana seeds as a novel potential agent against echinococcosis in experimentally protoscolex-infected mice Veterinary Research |
| title | Therapeutic and immunoregulatory effects of water-soluble alkaloids E2-a from Sophora moorcroftiana seeds as a novel potential agent against echinococcosis in experimentally protoscolex-infected mice |
| title_full | Therapeutic and immunoregulatory effects of water-soluble alkaloids E2-a from Sophora moorcroftiana seeds as a novel potential agent against echinococcosis in experimentally protoscolex-infected mice |
| title_fullStr | Therapeutic and immunoregulatory effects of water-soluble alkaloids E2-a from Sophora moorcroftiana seeds as a novel potential agent against echinococcosis in experimentally protoscolex-infected mice |
| title_full_unstemmed | Therapeutic and immunoregulatory effects of water-soluble alkaloids E2-a from Sophora moorcroftiana seeds as a novel potential agent against echinococcosis in experimentally protoscolex-infected mice |
| title_short | Therapeutic and immunoregulatory effects of water-soluble alkaloids E2-a from Sophora moorcroftiana seeds as a novel potential agent against echinococcosis in experimentally protoscolex-infected mice |
| title_sort | therapeutic and immunoregulatory effects of water soluble alkaloids e2 a from sophora moorcroftiana seeds as a novel potential agent against echinococcosis in experimentally protoscolex infected mice |
| url | http://link.springer.com/article/10.1186/s13567-018-0596-9 |
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