Activation of kinin B1 receptor evokes hyperthermia through a vagal sensory mechanism in the rat
<p>Abstract</p> <p>Background</p> <p>Kinins are mediators of pain and inflammation. Their role in thermoregulation is, however, unknown despite the fact the B1 receptor (B1R) was found implicated in lipopolysaccharide (LPS)-induced fever. The aim of this study was to in...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-09-01
|
| Series: | Journal of Neuroinflammation |
| Subjects: | |
| Online Access: | http://www.jneuroinflammation.com/content/9/1/214 |
| _version_ | 1818390955423694848 |
|---|---|
| author | Talbot Sébastien De Brito Gariépy Helaine Saint-Denis Julien Couture Réjean |
| author_facet | Talbot Sébastien De Brito Gariépy Helaine Saint-Denis Julien Couture Réjean |
| author_sort | Talbot Sébastien |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Kinins are mediators of pain and inflammation. Their role in thermoregulation is, however, unknown despite the fact the B1 receptor (B1R) was found implicated in lipopolysaccharide (LPS)-induced fever. The aim of this study was to investigate the mechanism by which peripheral B1R affects body core temperature in a rat model known to show up-regulated levels of B1R.</p> <p>Methods</p> <p>Male Sprague–Dawley rats received streptozotocin (STZ, 65 mg/kg; i.p.) to enhance B1R expression. Control rats received the vehicle only. One week later, rectal temperature was measured in awake rats after i.p. injection of increasing doses (0.01 to 5 mg/kg) of des-Arg<sup>9</sup>-Bradykinin (BK) and Sar-[D-Phe<sup>8</sup>]des-Arg<sup>9</sup>-BK (B1R agonists) or BK (B2R agonist). The mechanism of B1R-induced hyperthermia was addressed using specific inhibitors and in rats subjected to subdiaphragmatic vagal nerve ligation. B1R mRNA level was measured by quantitative Real Time-polymerase chain reaction (qRT-PCR) and B1R was localized by confocal microscopy.</p> <p>Results</p> <p>B1R agonists (0.1 to 5 mg/kg) showed transient (5- to 30-minute) and dose-dependent increases of rectal temperature (+1.5°C) in STZ-treated rats, but not in control rats. BK caused no effect in STZ and control rats. In STZ-treated rats, B1R agonist-induced hyperthermia was blocked by antagonists/inhibitors of B1R (SSR240612), cyclooxygenase-2 (COX-2) (niflumic acid) and nitric oxide synthase (NOS) (L-NAME), and after vagal nerve ligation. In contrast, COX-1 inhibition (indomethacin) had no effect on B1R agonist-induced hyperthermia. In STZ-treated rats, B1R mRNA was significantly increased in the hypothalamus and the vagus nerve where it was co-localized with calcitonin-gene-related peptide in sensory C-fibers.</p> <p>Conclusion</p> <p>B1R, which is induced in inflammatory diseases, could contribute to hyperthermia through a vagal sensory mechanism involving prostaglandins (via COX-2) and nitric oxide.</p> |
| first_indexed | 2024-12-14T05:05:51Z |
| format | Article |
| id | doaj.art-6f2aad77b315444f99bf01bd55fce87e |
| institution | Directory Open Access Journal |
| issn | 1742-2094 |
| language | English |
| last_indexed | 2024-12-14T05:05:51Z |
| publishDate | 2012-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Neuroinflammation |
| spelling | doaj.art-6f2aad77b315444f99bf01bd55fce87e2022-12-21T23:16:07ZengBMCJournal of Neuroinflammation1742-20942012-09-019121410.1186/1742-2094-9-214Activation of kinin B1 receptor evokes hyperthermia through a vagal sensory mechanism in the ratTalbot SébastienDe Brito Gariépy HelaineSaint-Denis JulienCouture Réjean<p>Abstract</p> <p>Background</p> <p>Kinins are mediators of pain and inflammation. Their role in thermoregulation is, however, unknown despite the fact the B1 receptor (B1R) was found implicated in lipopolysaccharide (LPS)-induced fever. The aim of this study was to investigate the mechanism by which peripheral B1R affects body core temperature in a rat model known to show up-regulated levels of B1R.</p> <p>Methods</p> <p>Male Sprague–Dawley rats received streptozotocin (STZ, 65 mg/kg; i.p.) to enhance B1R expression. Control rats received the vehicle only. One week later, rectal temperature was measured in awake rats after i.p. injection of increasing doses (0.01 to 5 mg/kg) of des-Arg<sup>9</sup>-Bradykinin (BK) and Sar-[D-Phe<sup>8</sup>]des-Arg<sup>9</sup>-BK (B1R agonists) or BK (B2R agonist). The mechanism of B1R-induced hyperthermia was addressed using specific inhibitors and in rats subjected to subdiaphragmatic vagal nerve ligation. B1R mRNA level was measured by quantitative Real Time-polymerase chain reaction (qRT-PCR) and B1R was localized by confocal microscopy.</p> <p>Results</p> <p>B1R agonists (0.1 to 5 mg/kg) showed transient (5- to 30-minute) and dose-dependent increases of rectal temperature (+1.5°C) in STZ-treated rats, but not in control rats. BK caused no effect in STZ and control rats. In STZ-treated rats, B1R agonist-induced hyperthermia was blocked by antagonists/inhibitors of B1R (SSR240612), cyclooxygenase-2 (COX-2) (niflumic acid) and nitric oxide synthase (NOS) (L-NAME), and after vagal nerve ligation. In contrast, COX-1 inhibition (indomethacin) had no effect on B1R agonist-induced hyperthermia. In STZ-treated rats, B1R mRNA was significantly increased in the hypothalamus and the vagus nerve where it was co-localized with calcitonin-gene-related peptide in sensory C-fibers.</p> <p>Conclusion</p> <p>B1R, which is induced in inflammatory diseases, could contribute to hyperthermia through a vagal sensory mechanism involving prostaglandins (via COX-2) and nitric oxide.</p>http://www.jneuroinflammation.com/content/9/1/214Kinin B1 receptorDiabetesHyperthermiaInflammationBradykininNOSensory C-fibersThermoregulation |
| spellingShingle | Talbot Sébastien De Brito Gariépy Helaine Saint-Denis Julien Couture Réjean Activation of kinin B1 receptor evokes hyperthermia through a vagal sensory mechanism in the rat Journal of Neuroinflammation Kinin B1 receptor Diabetes Hyperthermia Inflammation Bradykinin NO Sensory C-fibers Thermoregulation |
| title | Activation of kinin B1 receptor evokes hyperthermia through a vagal sensory mechanism in the rat |
| title_full | Activation of kinin B1 receptor evokes hyperthermia through a vagal sensory mechanism in the rat |
| title_fullStr | Activation of kinin B1 receptor evokes hyperthermia through a vagal sensory mechanism in the rat |
| title_full_unstemmed | Activation of kinin B1 receptor evokes hyperthermia through a vagal sensory mechanism in the rat |
| title_short | Activation of kinin B1 receptor evokes hyperthermia through a vagal sensory mechanism in the rat |
| title_sort | activation of kinin b1 receptor evokes hyperthermia through a vagal sensory mechanism in the rat |
| topic | Kinin B1 receptor Diabetes Hyperthermia Inflammation Bradykinin NO Sensory C-fibers Thermoregulation |
| url | http://www.jneuroinflammation.com/content/9/1/214 |
| work_keys_str_mv | AT talbotsebastien activationofkininb1receptorevokeshyperthermiathroughavagalsensorymechanismintherat AT debritogariepyhelaine activationofkininb1receptorevokeshyperthermiathroughavagalsensorymechanismintherat AT saintdenisjulien activationofkininb1receptorevokeshyperthermiathroughavagalsensorymechanismintherat AT couturerejean activationofkininb1receptorevokeshyperthermiathroughavagalsensorymechanismintherat |