Electron Beam Immobilization of Novel Antimicrobial, Short Peptide Motifs Leads to Membrane Surfaces with Promising Antibacterial Properties
In this study, the efficacy of electron beam irradiation versus chemical coupling for yielding polyethersulfone (PES) membranes with antibacterial properties was investigated. For the surface coating, a recently discovered lead compound, IL-KKA, comprising a short peptide sequence functionalized wit...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-02-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | http://www.mdpi.com/2079-4983/9/1/21 |
| _version_ | 1798004852612136960 |
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| author | André Reinhardt Isabell Thomas Julie Schmauck Ralf Giernoth Agnes Schulze Ines Neundorf |
| author_facet | André Reinhardt Isabell Thomas Julie Schmauck Ralf Giernoth Agnes Schulze Ines Neundorf |
| author_sort | André Reinhardt |
| collection | DOAJ |
| description | In this study, the efficacy of electron beam irradiation versus chemical coupling for yielding polyethersulfone (PES) membranes with antibacterial properties was investigated. For the surface coating, a recently discovered lead compound, IL-KKA, comprising a short peptide sequence functionalized with imidazolium groups, was used. For better integration within the membrane, several novel variants of IL-KKA were generated. Membrane immobilization was achieved using different doses of electron beam irradiation and NHS/EDC chemical coupling. Physicochemical characterization of the coated membranes was performed by water contact angle measurements, X-ray photoelectron spectroscopy, and scanning electron microscopy. Our results show that electron beam irradiation is as effective and gentle as chemical coupling using the NHS/EDC method. Moreover, it was demonstrated that the obtained membranes exhibit promising antibacterial activity against B. subtilis. In summary, the technique presented herein might be promising as a template for developing future anti-biofilm devices. |
| first_indexed | 2024-04-11T12:30:10Z |
| format | Article |
| id | doaj.art-6f2ede6b1a624e38a41966ac28348e41 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-04-11T12:30:10Z |
| publishDate | 2018-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-6f2ede6b1a624e38a41966ac28348e412022-12-22T04:23:47ZengMDPI AGJournal of Functional Biomaterials2079-49832018-02-01912110.3390/jfb9010021jfb9010021Electron Beam Immobilization of Novel Antimicrobial, Short Peptide Motifs Leads to Membrane Surfaces with Promising Antibacterial PropertiesAndré Reinhardt0Isabell Thomas1Julie Schmauck2Ralf Giernoth3Agnes Schulze4Ines Neundorf5Department of Chemistry, Biochemistry, University of Cologne, Zülpicher Str. 47a, D-50674 Cologne, GermanyLeibniz Institute of Surface Engineering, Permoserstr. 15, D-04318 Leipzig, GermanyDepartment of Chemistry, Organic Chemistry, University of Cologne, Greinstr. 4, D-50939 Cologne, GermanyDepartment of Chemistry, Organic Chemistry, University of Cologne, Greinstr. 4, D-50939 Cologne, GermanyLeibniz Institute of Surface Engineering, Permoserstr. 15, D-04318 Leipzig, GermanyDepartment of Chemistry, Biochemistry, University of Cologne, Zülpicher Str. 47a, D-50674 Cologne, GermanyIn this study, the efficacy of electron beam irradiation versus chemical coupling for yielding polyethersulfone (PES) membranes with antibacterial properties was investigated. For the surface coating, a recently discovered lead compound, IL-KKA, comprising a short peptide sequence functionalized with imidazolium groups, was used. For better integration within the membrane, several novel variants of IL-KKA were generated. Membrane immobilization was achieved using different doses of electron beam irradiation and NHS/EDC chemical coupling. Physicochemical characterization of the coated membranes was performed by water contact angle measurements, X-ray photoelectron spectroscopy, and scanning electron microscopy. Our results show that electron beam irradiation is as effective and gentle as chemical coupling using the NHS/EDC method. Moreover, it was demonstrated that the obtained membranes exhibit promising antibacterial activity against B. subtilis. In summary, the technique presented herein might be promising as a template for developing future anti-biofilm devices.http://www.mdpi.com/2079-4983/9/1/21biofilm formationelectron beamantimicrobial peptidessurface modificationimmobilization techniques |
| spellingShingle | André Reinhardt Isabell Thomas Julie Schmauck Ralf Giernoth Agnes Schulze Ines Neundorf Electron Beam Immobilization of Novel Antimicrobial, Short Peptide Motifs Leads to Membrane Surfaces with Promising Antibacterial Properties Journal of Functional Biomaterials biofilm formation electron beam antimicrobial peptides surface modification immobilization techniques |
| title | Electron Beam Immobilization of Novel Antimicrobial, Short Peptide Motifs Leads to Membrane Surfaces with Promising Antibacterial Properties |
| title_full | Electron Beam Immobilization of Novel Antimicrobial, Short Peptide Motifs Leads to Membrane Surfaces with Promising Antibacterial Properties |
| title_fullStr | Electron Beam Immobilization of Novel Antimicrobial, Short Peptide Motifs Leads to Membrane Surfaces with Promising Antibacterial Properties |
| title_full_unstemmed | Electron Beam Immobilization of Novel Antimicrobial, Short Peptide Motifs Leads to Membrane Surfaces with Promising Antibacterial Properties |
| title_short | Electron Beam Immobilization of Novel Antimicrobial, Short Peptide Motifs Leads to Membrane Surfaces with Promising Antibacterial Properties |
| title_sort | electron beam immobilization of novel antimicrobial short peptide motifs leads to membrane surfaces with promising antibacterial properties |
| topic | biofilm formation electron beam antimicrobial peptides surface modification immobilization techniques |
| url | http://www.mdpi.com/2079-4983/9/1/21 |
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