Development of new immunoanalytical test systems for diagnostics of potato blackleg caused by Dickeya spp. bacteria
Potato blackleg caused by Dickeya spp. bacteria is one of the most important bacterial diseases of potatoes. The rapid spread of this disease in the territory of Russia requires new effective diagnostic tools for the timely detection of infection. To solve this problem, antisera specific to Dickeya...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Peoples’ Friendship University of Russia (RUDN University)
2021-10-01
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| Series: | RUDN Journal of Agronomy and Animal Industries |
| Subjects: | |
| Online Access: | http://agrojournal.rudn.ru/agronomy/article/viewFile/19672/16276 |
| _version_ | 1818692949775482880 |
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| author | Shyatesa Razo Pavel A. Galushka Yuri A. Varitsev Anatoly V. Zherdev Irina V. Safenkova Elena N. Pakina Boris B. Dzantiev |
| author_facet | Shyatesa Razo Pavel A. Galushka Yuri A. Varitsev Anatoly V. Zherdev Irina V. Safenkova Elena N. Pakina Boris B. Dzantiev |
| author_sort | Shyatesa Razo |
| collection | DOAJ |
| description | Potato blackleg caused by Dickeya spp. bacteria is one of the most important bacterial diseases of potatoes. The rapid spread of this disease in the territory of Russia requires new effective diagnostic tools for the timely detection of infection. To solve this problem, antisera specific to Dickeya spp. were obtained. Polyclonal antibodies isolated from antisera have shown high affinity for the main species of Dickeya spp. ( D. solani, D. dianthicola, D. chrysanthemi, D. dadantii, D. paradisiaca ). Enzyme linked immunosorbent assay (ELISA) and lateral flow immunoassay (LFIA) test systems have been developed based on specific and high affinity antibodies that were obtained. For ELISA, the detection limit was 0.8 105 cells/mL for D. solani and 2 104 cells/mL for D. dianthicola . For LFIA, suitable for use in non-laboratory conditions, the detection limit of D. solani was 2 105 cells/mL and the analysis time was 15 minutes. When testing potato seed material, LFIA test system confirmed positive results of ELISA determination in 75 % of samples, and negative - in 100 % of samples. |
| first_indexed | 2024-12-17T13:05:55Z |
| format | Article |
| id | doaj.art-6f2f40916fe34dfca6ee0352c8ba1782 |
| institution | Directory Open Access Journal |
| issn | 2312-797X 2312-7988 |
| language | English |
| last_indexed | 2024-12-17T13:05:55Z |
| publishDate | 2021-10-01 |
| publisher | Peoples’ Friendship University of Russia (RUDN University) |
| record_format | Article |
| series | RUDN Journal of Agronomy and Animal Industries |
| spelling | doaj.art-6f2f40916fe34dfca6ee0352c8ba17822022-12-21T21:47:14ZengPeoples’ Friendship University of Russia (RUDN University)RUDN Journal of Agronomy and Animal Industries2312-797X2312-79882021-10-0116319821410.22363/2312-797X-2021-16-3-198-21416940Development of new immunoanalytical test systems for diagnostics of potato blackleg caused by Dickeya spp. bacteriaShyatesa Razo0Pavel A. Galushka1Yuri A. Varitsev2Anatoly V. Zherdev3Irina V. Safenkova4Elena N. Pakina5Boris B. Dzantiev6A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences; RUDN UniversityRussian Potato Research CenterRussian Potato Research CenterA.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of SciencesA.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of SciencesRUDN UniversityA.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of SciencesPotato blackleg caused by Dickeya spp. bacteria is one of the most important bacterial diseases of potatoes. The rapid spread of this disease in the territory of Russia requires new effective diagnostic tools for the timely detection of infection. To solve this problem, antisera specific to Dickeya spp. were obtained. Polyclonal antibodies isolated from antisera have shown high affinity for the main species of Dickeya spp. ( D. solani, D. dianthicola, D. chrysanthemi, D. dadantii, D. paradisiaca ). Enzyme linked immunosorbent assay (ELISA) and lateral flow immunoassay (LFIA) test systems have been developed based on specific and high affinity antibodies that were obtained. For ELISA, the detection limit was 0.8 105 cells/mL for D. solani and 2 104 cells/mL for D. dianthicola . For LFIA, suitable for use in non-laboratory conditions, the detection limit of D. solani was 2 105 cells/mL and the analysis time was 15 minutes. When testing potato seed material, LFIA test system confirmed positive results of ELISA determination in 75 % of samples, and negative - in 100 % of samples.http://agrojournal.rudn.ru/agronomy/article/viewFile/19672/16276dickeya sppenzyme linked immunosorbent assaylateral flow immunoassayphytopathogenspotato blacklegdickeya spp |
| spellingShingle | Shyatesa Razo Pavel A. Galushka Yuri A. Varitsev Anatoly V. Zherdev Irina V. Safenkova Elena N. Pakina Boris B. Dzantiev Development of new immunoanalytical test systems for diagnostics of potato blackleg caused by Dickeya spp. bacteria RUDN Journal of Agronomy and Animal Industries dickeya spp enzyme linked immunosorbent assay lateral flow immunoassay phytopathogens potato blackleg dickeya spp |
| title | Development of new immunoanalytical test systems for diagnostics of potato blackleg caused by Dickeya spp. bacteria |
| title_full | Development of new immunoanalytical test systems for diagnostics of potato blackleg caused by Dickeya spp. bacteria |
| title_fullStr | Development of new immunoanalytical test systems for diagnostics of potato blackleg caused by Dickeya spp. bacteria |
| title_full_unstemmed | Development of new immunoanalytical test systems for diagnostics of potato blackleg caused by Dickeya spp. bacteria |
| title_short | Development of new immunoanalytical test systems for diagnostics of potato blackleg caused by Dickeya spp. bacteria |
| title_sort | development of new immunoanalytical test systems for diagnostics of potato blackleg caused by dickeya spp bacteria |
| topic | dickeya spp enzyme linked immunosorbent assay lateral flow immunoassay phytopathogens potato blackleg dickeya spp |
| url | http://agrojournal.rudn.ru/agronomy/article/viewFile/19672/16276 |
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