Comparison of the Effect of Fertilization with Ash from Wood Chips on Bacterial Community in Podzolic and Chernozem Soils for the Cultivation of Winter Oilseed Rape: A Preliminary Study
The aim of the research was to investigate whether different doses of ash from biomass combustion (<i>Salix viminalis</i> L. willow) have an impact on the number and community of soil bacteria. The experiment was carried out on podzolic and chernozem soils in a one-way field experiment (...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/3/576 |
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| author | Jadwiga Stanek-Tarkowska Miłosz Pastuszczak Ewa Szpunar-Krok Miroslava Kačániová Maciej Ireneusz Kluz Ewa Antonina Czyż Rafał Pieniążek Karol Skrobacz Karol Pietrzyk |
| author_facet | Jadwiga Stanek-Tarkowska Miłosz Pastuszczak Ewa Szpunar-Krok Miroslava Kačániová Maciej Ireneusz Kluz Ewa Antonina Czyż Rafał Pieniążek Karol Skrobacz Karol Pietrzyk |
| author_sort | Jadwiga Stanek-Tarkowska |
| collection | DOAJ |
| description | The aim of the research was to investigate whether different doses of ash from biomass combustion (<i>Salix viminalis</i> L. willow) have an impact on the number and community of soil bacteria. The experiment was carried out on podzolic and chernozem soils in a one-way field experiment (control, NPK, 100, 200, 300, 400, 500 kg K<sub>2</sub>Oha<sup>−1</sup>). The ash from the biomass was characterized by pH 12.83 ± 0.68 and high content of macronutrients. Samples were taken from the 0–5 cm layer of soil under the cultivation of winter oilseed rape (<i>Brassica napus</i> L. var. Napus) in April and September 2021. The plate count method with PCA solid medium was used to determine the number of microorganisms, and mass spectrometry (MALDI-TOF MS) was used to analyze the microbiological community. The research showed an increase in the number of microorganisms after the use of the biomass ash fertilizer in the variants with ash doses from 200 to 500 kg K<sub>2</sub>Oha<sup>−1</sup>. The highest amount of soil bacteria in both tested soils was determined in these variants. In total, 44 bacterial species of 5 genera were identified in all variants: <i>Bacillus</i>, <i>Paenarthrobacter</i>, <i>Pseudarthrobacter</i>, <i>Pseudomonas</i>, and <i>Rhodococcus</i>. An important factor in the growth of the number of bacteria, in addition to the dose of biomass ash, was soil moisture, which in September was significantly higher than in April 2021 in both soils. |
| first_indexed | 2024-03-09T20:13:36Z |
| format | Article |
| id | doaj.art-6f2fa309c4a44940904f6c66b52cd625 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T20:13:36Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-6f2fa309c4a44940904f6c66b52cd6252023-11-24T00:07:11ZengMDPI AGAgronomy2073-43952022-02-0112357610.3390/agronomy12030576Comparison of the Effect of Fertilization with Ash from Wood Chips on Bacterial Community in Podzolic and Chernozem Soils for the Cultivation of Winter Oilseed Rape: A Preliminary StudyJadwiga Stanek-Tarkowska0Miłosz Pastuszczak1Ewa Szpunar-Krok2Miroslava Kačániová3Maciej Ireneusz Kluz4Ewa Antonina Czyż5Rafał Pieniążek6Karol Skrobacz7Karol Pietrzyk8Department of Soil Science, Environmental Chemistry and Hydrology, University of Rzeszow, Zelwerowicza 8B, 35-601 Rzeszów, PolandDepartment of Soil Science, Environmental Chemistry and Hydrology, University of Rzeszow, Zelwerowicza 8B, 35-601 Rzeszów, PolandDepartment of Plant Production, College of Natural Sciences, University of Rzeszów, Zelwerowicza 4, 35-601 Rzeszów, PolandDepartment of Bioenergetics, Food Analysis and Microbiology, Institute of Food and Nutrition Technology, University of Rzeszow, 35-959 Rzeszow, PolandDepartment of Bioenergetics, Food Analysis and Microbiology, Institute of Food and Nutrition Technology, University of Rzeszow, 35-959 Rzeszow, PolandDepartment of Soil Science, Environmental Chemistry and Hydrology, University of Rzeszow, Zelwerowicza 8B, 35-601 Rzeszów, PolandDepartment of Soil Science, Environmental Chemistry and Hydrology, University of Rzeszow, Zelwerowicza 8B, 35-601 Rzeszów, PolandDepartment of Soil Science, Environmental Chemistry and Hydrology, University of Rzeszow, Zelwerowicza 8B, 35-601 Rzeszów, PolandDepartment of Bioenergetics, Food Analysis and Microbiology, Institute of Food and Nutrition Technology, University of Rzeszow, 35-959 Rzeszow, PolandThe aim of the research was to investigate whether different doses of ash from biomass combustion (<i>Salix viminalis</i> L. willow) have an impact on the number and community of soil bacteria. The experiment was carried out on podzolic and chernozem soils in a one-way field experiment (control, NPK, 100, 200, 300, 400, 500 kg K<sub>2</sub>Oha<sup>−1</sup>). The ash from the biomass was characterized by pH 12.83 ± 0.68 and high content of macronutrients. Samples were taken from the 0–5 cm layer of soil under the cultivation of winter oilseed rape (<i>Brassica napus</i> L. var. Napus) in April and September 2021. The plate count method with PCA solid medium was used to determine the number of microorganisms, and mass spectrometry (MALDI-TOF MS) was used to analyze the microbiological community. The research showed an increase in the number of microorganisms after the use of the biomass ash fertilizer in the variants with ash doses from 200 to 500 kg K<sub>2</sub>Oha<sup>−1</sup>. The highest amount of soil bacteria in both tested soils was determined in these variants. In total, 44 bacterial species of 5 genera were identified in all variants: <i>Bacillus</i>, <i>Paenarthrobacter</i>, <i>Pseudarthrobacter</i>, <i>Pseudomonas</i>, and <i>Rhodococcus</i>. An important factor in the growth of the number of bacteria, in addition to the dose of biomass ash, was soil moisture, which in September was significantly higher than in April 2021 in both soils.https://www.mdpi.com/2073-4395/12/3/576biomass ashessoil moisturesoil bacteriamicroorganismsfertilizationMALDI-TOF MS Biotyper |
| spellingShingle | Jadwiga Stanek-Tarkowska Miłosz Pastuszczak Ewa Szpunar-Krok Miroslava Kačániová Maciej Ireneusz Kluz Ewa Antonina Czyż Rafał Pieniążek Karol Skrobacz Karol Pietrzyk Comparison of the Effect of Fertilization with Ash from Wood Chips on Bacterial Community in Podzolic and Chernozem Soils for the Cultivation of Winter Oilseed Rape: A Preliminary Study Agronomy biomass ashes soil moisture soil bacteria microorganisms fertilization MALDI-TOF MS Biotyper |
| title | Comparison of the Effect of Fertilization with Ash from Wood Chips on Bacterial Community in Podzolic and Chernozem Soils for the Cultivation of Winter Oilseed Rape: A Preliminary Study |
| title_full | Comparison of the Effect of Fertilization with Ash from Wood Chips on Bacterial Community in Podzolic and Chernozem Soils for the Cultivation of Winter Oilseed Rape: A Preliminary Study |
| title_fullStr | Comparison of the Effect of Fertilization with Ash from Wood Chips on Bacterial Community in Podzolic and Chernozem Soils for the Cultivation of Winter Oilseed Rape: A Preliminary Study |
| title_full_unstemmed | Comparison of the Effect of Fertilization with Ash from Wood Chips on Bacterial Community in Podzolic and Chernozem Soils for the Cultivation of Winter Oilseed Rape: A Preliminary Study |
| title_short | Comparison of the Effect of Fertilization with Ash from Wood Chips on Bacterial Community in Podzolic and Chernozem Soils for the Cultivation of Winter Oilseed Rape: A Preliminary Study |
| title_sort | comparison of the effect of fertilization with ash from wood chips on bacterial community in podzolic and chernozem soils for the cultivation of winter oilseed rape a preliminary study |
| topic | biomass ashes soil moisture soil bacteria microorganisms fertilization MALDI-TOF MS Biotyper |
| url | https://www.mdpi.com/2073-4395/12/3/576 |
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