Osteoblastogenesis Alters Small RNA Profiles in EVs Derived from Bone Marrow Stem Cells (BMSCs) and Adipose Stem Cells (ASCs)
Multipotent stem cells (MSCs) are used in various therapeutic applications based on their paracrine secretion activity. Here, we set out to identify and characterize the paracrine factors released during osteoblastogenesis, with a special focus on small non-coding RNAs released in extracellular vesi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/8/10/387 |
| _version_ | 1797552350774165504 |
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| author | Yan Yan Clare Chang Junyi Su Morten T. Venø Jørgen Kjems |
| author_facet | Yan Yan Clare Chang Junyi Su Morten T. Venø Jørgen Kjems |
| author_sort | Yan Yan |
| collection | DOAJ |
| description | Multipotent stem cells (MSCs) are used in various therapeutic applications based on their paracrine secretion activity. Here, we set out to identify and characterize the paracrine factors released during osteoblastogenesis, with a special focus on small non-coding RNAs released in extracellular vesicles (EVs). Bone marrow stem cells (BMSCs) and adipose stem cells (ASCs) from healthy human donors were used as representatives of MSCs. We isolated EVs secreted before and after induction of osteoblastic differentiation and found that the EVs contained a specific subset of microRNAs (miRNAs) and tRNA-derived small RNAs (tsRNA) compared to their parental cells. Osteoblastic differentiation had a larger effect on the small RNA profile of BMSC-EVs relative to ASC-EVs. Our data showed that EVs from different MSC origin exhibited distinct expression profiles of small RNA profiles when undergoing osteoblastogenesis, a factor that should be taken into consideration for stem cell therapy. |
| first_indexed | 2024-03-10T15:58:49Z |
| format | Article |
| id | doaj.art-6f3244d2e6aa433a936b414ceeb4a6ee |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T15:58:49Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-6f3244d2e6aa433a936b414ceeb4a6ee2023-11-20T15:26:41ZengMDPI AGBiomedicines2227-90592020-09-0181038710.3390/biomedicines8100387Osteoblastogenesis Alters Small RNA Profiles in EVs Derived from Bone Marrow Stem Cells (BMSCs) and Adipose Stem Cells (ASCs)Yan Yan0Clare Chang1Junyi Su2Morten T. Venø3Jørgen Kjems4Interdisciplinary Nanoscience Center, Aarhus University, 8000 Aarhus, DenmarkInterdisciplinary Nanoscience Center, Aarhus University, 8000 Aarhus, DenmarkInterdisciplinary Nanoscience Center, Aarhus University, 8000 Aarhus, DenmarkInterdisciplinary Nanoscience Center, Aarhus University, 8000 Aarhus, DenmarkInterdisciplinary Nanoscience Center, Aarhus University, 8000 Aarhus, DenmarkMultipotent stem cells (MSCs) are used in various therapeutic applications based on their paracrine secretion activity. Here, we set out to identify and characterize the paracrine factors released during osteoblastogenesis, with a special focus on small non-coding RNAs released in extracellular vesicles (EVs). Bone marrow stem cells (BMSCs) and adipose stem cells (ASCs) from healthy human donors were used as representatives of MSCs. We isolated EVs secreted before and after induction of osteoblastic differentiation and found that the EVs contained a specific subset of microRNAs (miRNAs) and tRNA-derived small RNAs (tsRNA) compared to their parental cells. Osteoblastic differentiation had a larger effect on the small RNA profile of BMSC-EVs relative to ASC-EVs. Our data showed that EVs from different MSC origin exhibited distinct expression profiles of small RNA profiles when undergoing osteoblastogenesis, a factor that should be taken into consideration for stem cell therapy.https://www.mdpi.com/2227-9059/8/10/387multipotent stem cellbone marrow stem celladipose stem cellsosteoblastogenesisextracellular vesiclesmicroRNA |
| spellingShingle | Yan Yan Clare Chang Junyi Su Morten T. Venø Jørgen Kjems Osteoblastogenesis Alters Small RNA Profiles in EVs Derived from Bone Marrow Stem Cells (BMSCs) and Adipose Stem Cells (ASCs) Biomedicines multipotent stem cell bone marrow stem cell adipose stem cells osteoblastogenesis extracellular vesicles microRNA |
| title | Osteoblastogenesis Alters Small RNA Profiles in EVs Derived from Bone Marrow Stem Cells (BMSCs) and Adipose Stem Cells (ASCs) |
| title_full | Osteoblastogenesis Alters Small RNA Profiles in EVs Derived from Bone Marrow Stem Cells (BMSCs) and Adipose Stem Cells (ASCs) |
| title_fullStr | Osteoblastogenesis Alters Small RNA Profiles in EVs Derived from Bone Marrow Stem Cells (BMSCs) and Adipose Stem Cells (ASCs) |
| title_full_unstemmed | Osteoblastogenesis Alters Small RNA Profiles in EVs Derived from Bone Marrow Stem Cells (BMSCs) and Adipose Stem Cells (ASCs) |
| title_short | Osteoblastogenesis Alters Small RNA Profiles in EVs Derived from Bone Marrow Stem Cells (BMSCs) and Adipose Stem Cells (ASCs) |
| title_sort | osteoblastogenesis alters small rna profiles in evs derived from bone marrow stem cells bmscs and adipose stem cells ascs |
| topic | multipotent stem cell bone marrow stem cell adipose stem cells osteoblastogenesis extracellular vesicles microRNA |
| url | https://www.mdpi.com/2227-9059/8/10/387 |
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