Expression analysis of plant intracellular Ras-group related leucine-rich repeat proteins (PIRLs) in Arabidopsis thaliana
Arabidopsis thaliana contains a family of nine genes known as plant intracellular Ras-group related leucine-rich repeat (LRR) proteins (PIRLs). These are structurally similar to animals and fungal LRR proteins and play important roles in developmental pathways. However, to date, no detailed tissue-s...
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| Format: | Članak |
| Jezik: | English |
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Elsevier
2022-07-01
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| Serija: | Biochemistry and Biophysics Reports |
| Teme: | |
| Online pristup: | http://www.sciencedirect.com/science/article/pii/S2405580822000413 |
| _version_ | 1828812172641173504 |
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| author | Md. Firose Hossain Mst Momtaz Sultana Ai Tanaka Amit Kumar Dutta Takushi Hachiya Tsuyoshi Nakagawa |
| author_facet | Md. Firose Hossain Mst Momtaz Sultana Ai Tanaka Amit Kumar Dutta Takushi Hachiya Tsuyoshi Nakagawa |
| author_sort | Md. Firose Hossain |
| collection | DOAJ |
| description | Arabidopsis thaliana contains a family of nine genes known as plant intracellular Ras-group related leucine-rich repeat (LRR) proteins (PIRLs). These are structurally similar to animals and fungal LRR proteins and play important roles in developmental pathways. However, to date, no detailed tissue-specific expression analysis of these PIRLs has been performed. Therefore, in this study, we generated promoter:GUS transgenic plants for the nine A. thaliana PIRL genes and identified their expression patterns in seedlings and floral organs at different developmental stages. Most PIRL members showed expression in the root apical region and in the vascular tissue of primary and lateral roots. Shoot apex-specific expression was recorded for PIRL1 and PIRL8. Furthermore, PIRL1, PIRL3, PIRL5, PIRL6, and PIRL7 showed distinct expression patterns in flowers, especially in pollen and anthers. In addition, co-expression network analysis identified cases where PIRLs were co-expressed with other genes known to have specific functions related to growth and development. Taken together, the tissue-specific expression patterns of PIRL genes improve our understanding of the functions of this gene family in plant growth and development. |
| first_indexed | 2024-12-12T09:39:31Z |
| format | Article |
| id | doaj.art-6f33c77c735e4313a0ff72e5cc3fc4b8 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-12T09:39:31Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-6f33c77c735e4313a0ff72e5cc3fc4b82022-12-22T00:28:37ZengElsevierBiochemistry and Biophysics Reports2405-58082022-07-0130101241Expression analysis of plant intracellular Ras-group related leucine-rich repeat proteins (PIRLs) in Arabidopsis thalianaMd. Firose Hossain0Mst Momtaz Sultana1Ai Tanaka2Amit Kumar Dutta3Takushi Hachiya4Tsuyoshi Nakagawa5Department of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Shimane University, Matsue, 690-8504, Japan; Bioresource and Life Sciences, The United Graduate School of Agricultural Sciences, Tottori University, Tottori, 680-8550, JapanDepartment of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Shimane University, Matsue, 690-8504, Japan; Department of Agricultural Extension (DAE), Ministry of Agriculture, Dhaka, 1215, BangladeshDepartment of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Shimane University, Matsue, 690-8504, JapanDepartment of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Shimane University, Matsue, 690-8504, Japan; Department of Microbiology, University of Rajshahi, Rajshahi, 6205, BangladeshDepartment of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Shimane University, Matsue, 690-8504, Japan; Bioresource and Life Sciences, The United Graduate School of Agricultural Sciences, Tottori University, Tottori, 680-8550, JapanDepartment of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Shimane University, Matsue, 690-8504, Japan; Bioresource and Life Sciences, The United Graduate School of Agricultural Sciences, Tottori University, Tottori, 680-8550, Japan; Corresponding author. Department of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Shimane University, Matsue, 690-8504, Japan.Arabidopsis thaliana contains a family of nine genes known as plant intracellular Ras-group related leucine-rich repeat (LRR) proteins (PIRLs). These are structurally similar to animals and fungal LRR proteins and play important roles in developmental pathways. However, to date, no detailed tissue-specific expression analysis of these PIRLs has been performed. Therefore, in this study, we generated promoter:GUS transgenic plants for the nine A. thaliana PIRL genes and identified their expression patterns in seedlings and floral organs at different developmental stages. Most PIRL members showed expression in the root apical region and in the vascular tissue of primary and lateral roots. Shoot apex-specific expression was recorded for PIRL1 and PIRL8. Furthermore, PIRL1, PIRL3, PIRL5, PIRL6, and PIRL7 showed distinct expression patterns in flowers, especially in pollen and anthers. In addition, co-expression network analysis identified cases where PIRLs were co-expressed with other genes known to have specific functions related to growth and development. Taken together, the tissue-specific expression patterns of PIRL genes improve our understanding of the functions of this gene family in plant growth and development.http://www.sciencedirect.com/science/article/pii/S2405580822000413Arabidopsis thalianaLeucine-rich repeatPromoter:GUS assayCo-expressionPhylogenetic analysisPollen |
| spellingShingle | Md. Firose Hossain Mst Momtaz Sultana Ai Tanaka Amit Kumar Dutta Takushi Hachiya Tsuyoshi Nakagawa Expression analysis of plant intracellular Ras-group related leucine-rich repeat proteins (PIRLs) in Arabidopsis thaliana Biochemistry and Biophysics Reports Arabidopsis thaliana Leucine-rich repeat Promoter:GUS assay Co-expression Phylogenetic analysis Pollen |
| title | Expression analysis of plant intracellular Ras-group related leucine-rich repeat proteins (PIRLs) in Arabidopsis thaliana |
| title_full | Expression analysis of plant intracellular Ras-group related leucine-rich repeat proteins (PIRLs) in Arabidopsis thaliana |
| title_fullStr | Expression analysis of plant intracellular Ras-group related leucine-rich repeat proteins (PIRLs) in Arabidopsis thaliana |
| title_full_unstemmed | Expression analysis of plant intracellular Ras-group related leucine-rich repeat proteins (PIRLs) in Arabidopsis thaliana |
| title_short | Expression analysis of plant intracellular Ras-group related leucine-rich repeat proteins (PIRLs) in Arabidopsis thaliana |
| title_sort | expression analysis of plant intracellular ras group related leucine rich repeat proteins pirls in arabidopsis thaliana |
| topic | Arabidopsis thaliana Leucine-rich repeat Promoter:GUS assay Co-expression Phylogenetic analysis Pollen |
| url | http://www.sciencedirect.com/science/article/pii/S2405580822000413 |
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