Rapid recovery of cyanobacterial pigments in desiccated biological soil crusts following addition of water.
We examined soil surface colour change to green and hydrotaxis following addition of water to biological soil crusts using pigment extraction, hyperspectral imaging, microsensors and 13C labeling experiments coupled to matrix-assisted laser desorption and ionization time of flight-mass spectrometry...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4223047?pdf=render |
| _version_ | 1819050682777337856 |
|---|---|
| author | Raeid M M Abed Lubos Polerecky Amal Al-Habsi Janina Oetjen Marc Strous Dirk de Beer |
| author_facet | Raeid M M Abed Lubos Polerecky Amal Al-Habsi Janina Oetjen Marc Strous Dirk de Beer |
| author_sort | Raeid M M Abed |
| collection | DOAJ |
| description | We examined soil surface colour change to green and hydrotaxis following addition of water to biological soil crusts using pigment extraction, hyperspectral imaging, microsensors and 13C labeling experiments coupled to matrix-assisted laser desorption and ionization time of flight-mass spectrometry (MALD-TOF MS). The topsoil colour turned green in less than 5 minutes following water addition. The concentrations of chlorophyll a (Chl a), scytonemin and echinenon rapidly increased in the top <1 mm layer while in the deeper layer, their concentrations remained low. Hyperspectral imaging showed that, in both wet and dehydrated crusts, cyanobacteria formed a layer at a depth of 0.2-0.4 mm and this layer did not move upward after wetting. 13C labeling experiments and MALDI TOF analysis showed that Chl a was already present in the desiccated crusts and de novo synthesis of this molecule started only after 2 days of wetting due to growth of cyanobacteria. Microsensor measurements showed that photosynthetic activity increased concomitantly with the increase of Chl a, and reached a maximum net rate of 92 µmol m-2 h-1 approximately 2 hours after wetting. We conclude that the colour change of soil crusts to green upon water addition was not due to hydrotaxis but rather to the quick recovery and reassembly of pigments. Cyanobacteria in crusts can maintain their photosynthetic apparatus intact even under prolonged periods of desiccation with the ability to resume their photosynthetic activities within minutes after wetting. |
| first_indexed | 2024-12-21T11:51:56Z |
| format | Article |
| id | doaj.art-6f345012d0524a2492a54672c2cc1907 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T11:51:56Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6f345012d0524a2492a54672c2cc19072022-12-21T19:05:02ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11237210.1371/journal.pone.0112372Rapid recovery of cyanobacterial pigments in desiccated biological soil crusts following addition of water.Raeid M M AbedLubos PolereckyAmal Al-HabsiJanina OetjenMarc StrousDirk de BeerWe examined soil surface colour change to green and hydrotaxis following addition of water to biological soil crusts using pigment extraction, hyperspectral imaging, microsensors and 13C labeling experiments coupled to matrix-assisted laser desorption and ionization time of flight-mass spectrometry (MALD-TOF MS). The topsoil colour turned green in less than 5 minutes following water addition. The concentrations of chlorophyll a (Chl a), scytonemin and echinenon rapidly increased in the top <1 mm layer while in the deeper layer, their concentrations remained low. Hyperspectral imaging showed that, in both wet and dehydrated crusts, cyanobacteria formed a layer at a depth of 0.2-0.4 mm and this layer did not move upward after wetting. 13C labeling experiments and MALDI TOF analysis showed that Chl a was already present in the desiccated crusts and de novo synthesis of this molecule started only after 2 days of wetting due to growth of cyanobacteria. Microsensor measurements showed that photosynthetic activity increased concomitantly with the increase of Chl a, and reached a maximum net rate of 92 µmol m-2 h-1 approximately 2 hours after wetting. We conclude that the colour change of soil crusts to green upon water addition was not due to hydrotaxis but rather to the quick recovery and reassembly of pigments. Cyanobacteria in crusts can maintain their photosynthetic apparatus intact even under prolonged periods of desiccation with the ability to resume their photosynthetic activities within minutes after wetting.http://europepmc.org/articles/PMC4223047?pdf=render |
| spellingShingle | Raeid M M Abed Lubos Polerecky Amal Al-Habsi Janina Oetjen Marc Strous Dirk de Beer Rapid recovery of cyanobacterial pigments in desiccated biological soil crusts following addition of water. PLoS ONE |
| title | Rapid recovery of cyanobacterial pigments in desiccated biological soil crusts following addition of water. |
| title_full | Rapid recovery of cyanobacterial pigments in desiccated biological soil crusts following addition of water. |
| title_fullStr | Rapid recovery of cyanobacterial pigments in desiccated biological soil crusts following addition of water. |
| title_full_unstemmed | Rapid recovery of cyanobacterial pigments in desiccated biological soil crusts following addition of water. |
| title_short | Rapid recovery of cyanobacterial pigments in desiccated biological soil crusts following addition of water. |
| title_sort | rapid recovery of cyanobacterial pigments in desiccated biological soil crusts following addition of water |
| url | http://europepmc.org/articles/PMC4223047?pdf=render |
| work_keys_str_mv | AT raeidmmabed rapidrecoveryofcyanobacterialpigmentsindesiccatedbiologicalsoilcrustsfollowingadditionofwater AT lubospolerecky rapidrecoveryofcyanobacterialpigmentsindesiccatedbiologicalsoilcrustsfollowingadditionofwater AT amalalhabsi rapidrecoveryofcyanobacterialpigmentsindesiccatedbiologicalsoilcrustsfollowingadditionofwater AT janinaoetjen rapidrecoveryofcyanobacterialpigmentsindesiccatedbiologicalsoilcrustsfollowingadditionofwater AT marcstrous rapidrecoveryofcyanobacterialpigmentsindesiccatedbiologicalsoilcrustsfollowingadditionofwater AT dirkdebeer rapidrecoveryofcyanobacterialpigmentsindesiccatedbiologicalsoilcrustsfollowingadditionofwater |