Itm2a is a Pax3 target gene, expressed at sites of skeletal muscle formation in vivo.
The paired-box homeodomain transcription factor Pax3 is a key regulator of the nervous system, neural crest and skeletal muscle development. Despite the important role of this transcription factor, very few direct target genes have been characterized. We show that Itm2a, which encodes a type 2 trans...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3641095?pdf=render |
| _version_ | 1818309747881803776 |
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| author | Mounia Lagha Alicia Mayeuf-Louchart Ted Chang Didier Montarras Didier Rocancourt Antoine Zalc Jay Kormish Kenneth S Zaret Margaret E Buckingham Frederic Relaix |
| author_facet | Mounia Lagha Alicia Mayeuf-Louchart Ted Chang Didier Montarras Didier Rocancourt Antoine Zalc Jay Kormish Kenneth S Zaret Margaret E Buckingham Frederic Relaix |
| author_sort | Mounia Lagha |
| collection | DOAJ |
| description | The paired-box homeodomain transcription factor Pax3 is a key regulator of the nervous system, neural crest and skeletal muscle development. Despite the important role of this transcription factor, very few direct target genes have been characterized. We show that Itm2a, which encodes a type 2 transmembrane protein, is a direct Pax3 target in vivo, by combining genetic approaches and in vivo chromatin immunoprecipitation assays. We have generated a conditional mutant allele for Itm2a, which is an imprinted gene, by flanking exons 2-4 with loxP sites and inserting an IRESnLacZ reporter in the 3' UTR of the gene. The LacZ reporter reproduces the expression profile of Itm2a, and allowed us to further characterize its expression at sites of myogenesis, in the dermomyotome and myotome of somites, and in limb buds, in the mouse embryo. We further show that Itm2a is not only expressed in adult muscle fibres but also in the satellite cells responsible for regeneration. Itm2a mutant mice are viable and fertile with no overt phenotype during skeletal muscle formation or regeneration. Potential compensatory mechanisms are discussed. |
| first_indexed | 2024-12-13T07:35:05Z |
| format | Article |
| id | doaj.art-6f367ee861654522a5bfbfbd3c2986b4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T07:35:05Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6f367ee861654522a5bfbfbd3c2986b42022-12-21T23:55:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0185e6314310.1371/journal.pone.0063143Itm2a is a Pax3 target gene, expressed at sites of skeletal muscle formation in vivo.Mounia LaghaAlicia Mayeuf-LouchartTed ChangDidier MontarrasDidier RocancourtAntoine ZalcJay KormishKenneth S ZaretMargaret E BuckinghamFrederic RelaixThe paired-box homeodomain transcription factor Pax3 is a key regulator of the nervous system, neural crest and skeletal muscle development. Despite the important role of this transcription factor, very few direct target genes have been characterized. We show that Itm2a, which encodes a type 2 transmembrane protein, is a direct Pax3 target in vivo, by combining genetic approaches and in vivo chromatin immunoprecipitation assays. We have generated a conditional mutant allele for Itm2a, which is an imprinted gene, by flanking exons 2-4 with loxP sites and inserting an IRESnLacZ reporter in the 3' UTR of the gene. The LacZ reporter reproduces the expression profile of Itm2a, and allowed us to further characterize its expression at sites of myogenesis, in the dermomyotome and myotome of somites, and in limb buds, in the mouse embryo. We further show that Itm2a is not only expressed in adult muscle fibres but also in the satellite cells responsible for regeneration. Itm2a mutant mice are viable and fertile with no overt phenotype during skeletal muscle formation or regeneration. Potential compensatory mechanisms are discussed.http://europepmc.org/articles/PMC3641095?pdf=render |
| spellingShingle | Mounia Lagha Alicia Mayeuf-Louchart Ted Chang Didier Montarras Didier Rocancourt Antoine Zalc Jay Kormish Kenneth S Zaret Margaret E Buckingham Frederic Relaix Itm2a is a Pax3 target gene, expressed at sites of skeletal muscle formation in vivo. PLoS ONE |
| title | Itm2a is a Pax3 target gene, expressed at sites of skeletal muscle formation in vivo. |
| title_full | Itm2a is a Pax3 target gene, expressed at sites of skeletal muscle formation in vivo. |
| title_fullStr | Itm2a is a Pax3 target gene, expressed at sites of skeletal muscle formation in vivo. |
| title_full_unstemmed | Itm2a is a Pax3 target gene, expressed at sites of skeletal muscle formation in vivo. |
| title_short | Itm2a is a Pax3 target gene, expressed at sites of skeletal muscle formation in vivo. |
| title_sort | itm2a is a pax3 target gene expressed at sites of skeletal muscle formation in vivo |
| url | http://europepmc.org/articles/PMC3641095?pdf=render |
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