Detection of plasma exosomal miRNA-205 as a biomarker for early diagnosis and an adjuvant indicator of ovarian cancer staging
Abstract Background Ovarian cancer (OC) is one of the serious threats to the health of women worldwide, and accurate biomarkers are urgently demanded for early diagnosis of OC. We have previously confirmed that miR-205 promotes the invasion and metastasis of OC cells by inhibiting the expression of...
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BMC
2022-02-01
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Series: | Journal of Ovarian Research |
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Online Access: | https://doi.org/10.1186/s13048-022-00961-x |
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author | Zehua Zhu Zhaojun Chen Mingxing Wang Min Zhang Yiwen Chen Xiao Yang Changjun Zhou Yuhua Liu Liquan Hong Lahong Zhang |
author_facet | Zehua Zhu Zhaojun Chen Mingxing Wang Min Zhang Yiwen Chen Xiao Yang Changjun Zhou Yuhua Liu Liquan Hong Lahong Zhang |
author_sort | Zehua Zhu |
collection | DOAJ |
description | Abstract Background Ovarian cancer (OC) is one of the serious threats to the health of women worldwide, and accurate biomarkers are urgently demanded for early diagnosis of OC. We have previously confirmed that miR-205 promotes the invasion and metastasis of OC cells by inhibiting the expression of the tumor suppressor gene TCF21. In this study, we used liquid biopsy technology to detect the expression levels of the four genes, miR-205, CA125, HE4 and TCF21, in the exosomes of plasma of OC patients. Combined with analysis of clinicopathological parameters of OC patients, we aimed to provide efficient and non-invasive laboratory biomarkers for early diagnosis of OC. Methods 36 OC patients who were diagnosed in local hospitals from September 2020 to July 2021 were selected as OC group, 31 cases of surgically diagnosed with ovarian benign lesions were selected as benign group, and 32 healthy people who underwent physical examination during the same period were selected as a control group. We employed transmission electron microscope (TEM), Western blotting (WB), and nanoparticle tracking analysis (NTA) to identify biomarkers in the exosomes extracted from plasma of the three groups. The RNA levels of miR-205, CA125, HE4 and TCF21 genes in plasma exosomes were detected by real-time quantitative PCR (qRT-PCR) method. We used clinical pathological parameters and the Receiver Operating Characteristic (ROC) curves to evaluate the diagnostic efficacy for the genes detected in plasma exosomes. Results We found that the expression level of miR-205 in plasma exosomes of the OC group was significantly higher than that of the benign and control groups (P < 0.05), and the level of miR-205 was elevated during the III-IV periods of OC and lymph node metastasis. Conclusion The level of miR-205 in plasma exosomes is a valuable tumor biomarker to improve OC diagnosis. |
first_indexed | 2024-04-11T02:28:06Z |
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institution | Directory Open Access Journal |
issn | 1757-2215 |
language | English |
last_indexed | 2024-04-11T02:28:06Z |
publishDate | 2022-02-01 |
publisher | BMC |
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series | Journal of Ovarian Research |
spelling | doaj.art-6f39ec1172484a6e9881a9a76ce52bd62023-01-02T22:12:44ZengBMCJournal of Ovarian Research1757-22152022-02-0115111010.1186/s13048-022-00961-xDetection of plasma exosomal miRNA-205 as a biomarker for early diagnosis and an adjuvant indicator of ovarian cancer stagingZehua Zhu0Zhaojun Chen1Mingxing Wang2Min Zhang3Yiwen Chen4Xiao Yang5Changjun Zhou6Yuhua Liu7Liquan Hong8Lahong Zhang9The Medical School of Hangzhou Normal UniversityThe Clinical laboratory of The Affiliated Hospital of Hangzhou Normal UniversityThe Medical School of Hangzhou Normal UniversityInstitute of Basic Medicine and Cancer of The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital)The Clinical laboratory of The Affiliated Hospital of Hangzhou Normal UniversityThe Clinical laboratory of The Affiliated Hospital of Hangzhou Normal UniversityThe Clinical laboratory of The Affiliated Hospital of Hangzhou Normal UniversityThe Clinical laboratory of The Affiliated Hospital of Hangzhou Normal UniversityThe Clinical laboratory of The Affiliated Hospital of Hangzhou Normal UniversityThe Clinical laboratory of The Affiliated Hospital of Hangzhou Normal UniversityAbstract Background Ovarian cancer (OC) is one of the serious threats to the health of women worldwide, and accurate biomarkers are urgently demanded for early diagnosis of OC. We have previously confirmed that miR-205 promotes the invasion and metastasis of OC cells by inhibiting the expression of the tumor suppressor gene TCF21. In this study, we used liquid biopsy technology to detect the expression levels of the four genes, miR-205, CA125, HE4 and TCF21, in the exosomes of plasma of OC patients. Combined with analysis of clinicopathological parameters of OC patients, we aimed to provide efficient and non-invasive laboratory biomarkers for early diagnosis of OC. Methods 36 OC patients who were diagnosed in local hospitals from September 2020 to July 2021 were selected as OC group, 31 cases of surgically diagnosed with ovarian benign lesions were selected as benign group, and 32 healthy people who underwent physical examination during the same period were selected as a control group. We employed transmission electron microscope (TEM), Western blotting (WB), and nanoparticle tracking analysis (NTA) to identify biomarkers in the exosomes extracted from plasma of the three groups. The RNA levels of miR-205, CA125, HE4 and TCF21 genes in plasma exosomes were detected by real-time quantitative PCR (qRT-PCR) method. We used clinical pathological parameters and the Receiver Operating Characteristic (ROC) curves to evaluate the diagnostic efficacy for the genes detected in plasma exosomes. Results We found that the expression level of miR-205 in plasma exosomes of the OC group was significantly higher than that of the benign and control groups (P < 0.05), and the level of miR-205 was elevated during the III-IV periods of OC and lymph node metastasis. Conclusion The level of miR-205 in plasma exosomes is a valuable tumor biomarker to improve OC diagnosis.https://doi.org/10.1186/s13048-022-00961-xOvarian cancerExosomemiRNA-205DiagnosisBiomarker |
spellingShingle | Zehua Zhu Zhaojun Chen Mingxing Wang Min Zhang Yiwen Chen Xiao Yang Changjun Zhou Yuhua Liu Liquan Hong Lahong Zhang Detection of plasma exosomal miRNA-205 as a biomarker for early diagnosis and an adjuvant indicator of ovarian cancer staging Journal of Ovarian Research Ovarian cancer Exosome miRNA-205 Diagnosis Biomarker |
title | Detection of plasma exosomal miRNA-205 as a biomarker for early diagnosis and an adjuvant indicator of ovarian cancer staging |
title_full | Detection of plasma exosomal miRNA-205 as a biomarker for early diagnosis and an adjuvant indicator of ovarian cancer staging |
title_fullStr | Detection of plasma exosomal miRNA-205 as a biomarker for early diagnosis and an adjuvant indicator of ovarian cancer staging |
title_full_unstemmed | Detection of plasma exosomal miRNA-205 as a biomarker for early diagnosis and an adjuvant indicator of ovarian cancer staging |
title_short | Detection of plasma exosomal miRNA-205 as a biomarker for early diagnosis and an adjuvant indicator of ovarian cancer staging |
title_sort | detection of plasma exosomal mirna 205 as a biomarker for early diagnosis and an adjuvant indicator of ovarian cancer staging |
topic | Ovarian cancer Exosome miRNA-205 Diagnosis Biomarker |
url | https://doi.org/10.1186/s13048-022-00961-x |
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