Genome-Wide Analysis of BES1 Transcription Factor Family in Sorghum [Sorghum bicolor (L.) Moench] Genome
The BES1 transcription factor family has an important role in the biosynthesis of brassinosteroids. It is a steroid hormone that affects plant growth and development processes and the mechanism of response to environmental stresses. The aim of this study was to determine the expression profiles of...
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| Format: | Article |
| Language: | English |
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Siirt University
2020-02-01
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| Series: | Türkiye Tarımsal Araştırmalar Dergisi |
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| Online Access: | https://dergipark.org.tr/tr/pub/tutad/issue/51508/671605 |
| _version_ | 1828014753195229184 |
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| author | Ayşe Gül KASAPOĞLU Emre İLHAN Damla KIZILKAYA Arash HOSSEIN-POUR Kamil HALİLOĞLU |
| author_facet | Ayşe Gül KASAPOĞLU Emre İLHAN Damla KIZILKAYA Arash HOSSEIN-POUR Kamil HALİLOĞLU |
| author_sort | Ayşe Gül KASAPOĞLU |
| collection | DOAJ |
| description | The BES1 transcription factor family has an important role in the biosynthesis of brassinosteroids. It is a steroid
hormone that affects plant growth and development processes and the mechanism of response to environmental stresses. The
aim of this study was to determine the expression profiles of BES1 transcription factor in different tissues of sorghum
[Sorghum bicolor (L.) Moench] by applying different nitrogen sources (controlled fertilized, ammonia, nitrate and urea) and
to characterize BES1 gene family members through in silico methods. In sorghum genome, 9 Sobic-BES1 proteins with
amino acid numbers ranged from 190 and 716, molecular weights between 35.27 and 80.54 kDa and isoelectric points
between 5.0 and 10.07 were determined. In gene structure analysis, the number of estimated exons ranged from 2 to 10.
Phylogenetic relationship was determined using BES1 proteins of S. bicolor, Arabidopsis thaliana and Oryza sativa species.
Sobic-BES1-4 and Sobic-BES1-9 genes were found to be segmental duplicated in the evolutionary process. According to the silico gene expression analysis, the expression levels of Sobic-BES1-4 and -9 genes were the highest in the root and shoot tissues where different nitrogen sources and irrigation control were applied. On the other hand, the expression levels of Sobic-BES1-1, Sobic-BES1-2 and Sobic-BES1-8 genes were differed according to the nitrogen source and tissue used. The results of this study will provide a basis for functional gene research and contribute to the understanding of the BES1 gene family in Sorghum bicolor. |
| first_indexed | 2024-04-10T10:06:42Z |
| format | Article |
| id | doaj.art-6f3a2daead684c83bf47f476946149c9 |
| institution | Directory Open Access Journal |
| issn | 2148-2306 2528-858X |
| language | English |
| last_indexed | 2024-04-10T10:06:42Z |
| publishDate | 2020-02-01 |
| publisher | Siirt University |
| record_format | Article |
| series | Türkiye Tarımsal Araştırmalar Dergisi |
| spelling | doaj.art-6f3a2daead684c83bf47f476946149c92023-02-15T16:22:27ZengSiirt UniversityTürkiye Tarımsal Araştırmalar Dergisi2148-23062528-858X2020-02-0171859510.19159/tutad.671605Genome-Wide Analysis of BES1 Transcription Factor Family in Sorghum [Sorghum bicolor (L.) Moench] GenomeAyşe Gül KASAPOĞLU0https://orcid.org/0000-0002-6447-4921Emre İLHAN1https://orcid.org/0000-0002-8404-7900Damla KIZILKAYA2https://orcid.org/0000-0002-4967-1249Arash HOSSEIN-POUR3https://orcid.org/0000-0003-2611-8034Kamil HALİLOĞLU4https://orcid.org/0000-0002-4014-491XErzurum Teknik Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Erzurum, TÜRKİYEErzurum Teknik Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Erzurum, TÜRKİYEAnkara Yıldırım Beyazıt Üniversitesi, Geleneksel ve Tamamlayıcı Tıp Araştırma ve Uygulama Merkezi, Ankara, TÜRKİYEErdabil Tarımsal ve Doğal Kaynaklar Araştırma ve Eğitim Merkezi, Moghan Tarla ve Bahçe Bilimleri Araştırma Bölümü, Erdabil, İRANAtatürk Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Erzurum, TÜRKİYEThe BES1 transcription factor family has an important role in the biosynthesis of brassinosteroids. It is a steroid hormone that affects plant growth and development processes and the mechanism of response to environmental stresses. The aim of this study was to determine the expression profiles of BES1 transcription factor in different tissues of sorghum [Sorghum bicolor (L.) Moench] by applying different nitrogen sources (controlled fertilized, ammonia, nitrate and urea) and to characterize BES1 gene family members through in silico methods. In sorghum genome, 9 Sobic-BES1 proteins with amino acid numbers ranged from 190 and 716, molecular weights between 35.27 and 80.54 kDa and isoelectric points between 5.0 and 10.07 were determined. In gene structure analysis, the number of estimated exons ranged from 2 to 10. Phylogenetic relationship was determined using BES1 proteins of S. bicolor, Arabidopsis thaliana and Oryza sativa species. Sobic-BES1-4 and Sobic-BES1-9 genes were found to be segmental duplicated in the evolutionary process. According to the silico gene expression analysis, the expression levels of Sobic-BES1-4 and -9 genes were the highest in the root and shoot tissues where different nitrogen sources and irrigation control were applied. On the other hand, the expression levels of Sobic-BES1-1, Sobic-BES1-2 and Sobic-BES1-8 genes were differed according to the nitrogen source and tissue used. The results of this study will provide a basis for functional gene research and contribute to the understanding of the BES1 gene family in Sorghum bicolor.https://dergipark.org.tr/tr/pub/tutad/issue/51508/671605bioinformatic analysisphytohormonebrassinosteroidsgene familygene expression |
| spellingShingle | Ayşe Gül KASAPOĞLU Emre İLHAN Damla KIZILKAYA Arash HOSSEIN-POUR Kamil HALİLOĞLU Genome-Wide Analysis of BES1 Transcription Factor Family in Sorghum [Sorghum bicolor (L.) Moench] Genome Türkiye Tarımsal Araştırmalar Dergisi bioinformatic analysis phytohormone brassinosteroids gene family gene expression |
| title | Genome-Wide Analysis of BES1 Transcription Factor Family in Sorghum [Sorghum bicolor (L.) Moench] Genome |
| title_full | Genome-Wide Analysis of BES1 Transcription Factor Family in Sorghum [Sorghum bicolor (L.) Moench] Genome |
| title_fullStr | Genome-Wide Analysis of BES1 Transcription Factor Family in Sorghum [Sorghum bicolor (L.) Moench] Genome |
| title_full_unstemmed | Genome-Wide Analysis of BES1 Transcription Factor Family in Sorghum [Sorghum bicolor (L.) Moench] Genome |
| title_short | Genome-Wide Analysis of BES1 Transcription Factor Family in Sorghum [Sorghum bicolor (L.) Moench] Genome |
| title_sort | genome wide analysis of bes1 transcription factor family in sorghum sorghum bicolor l moench genome |
| topic | bioinformatic analysis phytohormone brassinosteroids gene family gene expression |
| url | https://dergipark.org.tr/tr/pub/tutad/issue/51508/671605 |
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