A Healthy Vaginal Microbiota Remains Stable during Oral Probiotic Supplementation: A Randomised Controlled Trial
The primary objective of this randomised, placebo-controlled, triple-blind study was to assess whether orally consumed <i>Lactobacillus acidophilus</i> La-14 (La-14) and <i>Lacticaseibacillus rhamnosus</i> HN001 (HN001) colonise a healthy human vagina. Furthermore, potential...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/2/499 |
| _version_ | 1797619161705218048 |
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| author | Anna Lyra Reeta Ala-Jaakkola Nicolas Yeung Neeta Datta Kara Evans Ashley Hibberd Markus J. Lehtinen Sofia D. Forssten Alvin Ibarra Tommi Pesonen Jouni Junnila Arthur C. Ouwehand Keith Baranowski Johanna Maukonen Gordon Crawford Liisa Lehtoranta |
| author_facet | Anna Lyra Reeta Ala-Jaakkola Nicolas Yeung Neeta Datta Kara Evans Ashley Hibberd Markus J. Lehtinen Sofia D. Forssten Alvin Ibarra Tommi Pesonen Jouni Junnila Arthur C. Ouwehand Keith Baranowski Johanna Maukonen Gordon Crawford Liisa Lehtoranta |
| author_sort | Anna Lyra |
| collection | DOAJ |
| description | The primary objective of this randomised, placebo-controlled, triple-blind study was to assess whether orally consumed <i>Lactobacillus acidophilus</i> La-14 (La-14) and <i>Lacticaseibacillus rhamnosus</i> HN001 (HN001) colonise a healthy human vagina. Furthermore, potential effects on vaginal microbiota and immune markers were explored. Fifty women devoid of vaginal complaints (Nugent score 0–3 and vaginal pH ≤ 4.5) were randomised into a 2-week intervention with either La-14 and HN001 as the verum product or a comparable placebo. Vaginal swab samples were collected at baseline, after one and two weeks of intervention, and after a one-week follow-up, for assessing colonisation of the supplemented lactobacilli, vaginal microbiota, and six specific immune markers. Colonisation of <i>L. acidophilus</i> and <i>L. rhamnosus</i> was not observed above the assay detection limit (5.29 and 5.11 log 10 genomes/swab for <i>L. acidophilus</i> and <i>L. rhamnosus</i>, respectively). Vaginal microbiotas remained stable and predominated by lactobacilli throughout the intervention, and vaginal pH remained optimal (at least 90% of participants in both groups had pH 4.0 or 4.5 throughout the study). Immune markers elafin and human β-defensin 3 (HBD-3) were significantly decreased in the verum group (<i>p</i> = 0.022 and <i>p</i> = 0.028, respectively) but did not correlate with any microbiota changes. Adverse events raised no safety concerns, and no undesired changes in the vaginal microbiota or immune markers were detected. |
| first_indexed | 2024-03-11T08:23:09Z |
| format | Article |
| id | doaj.art-6f3b64dc70a14c0b9d4312e3d13fbd12 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T08:23:09Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-6f3b64dc70a14c0b9d4312e3d13fbd122023-11-16T22:16:44ZengMDPI AGMicroorganisms2076-26072023-02-0111249910.3390/microorganisms11020499A Healthy Vaginal Microbiota Remains Stable during Oral Probiotic Supplementation: A Randomised Controlled TrialAnna Lyra0Reeta Ala-Jaakkola1Nicolas Yeung2Neeta Datta3Kara Evans4Ashley Hibberd5Markus J. Lehtinen6Sofia D. Forssten7Alvin Ibarra8Tommi Pesonen9Jouni Junnila10Arthur C. Ouwehand11Keith Baranowski12Johanna Maukonen13Gordon Crawford14Liisa Lehtoranta15IFF Health & Biosciences, 02460 Kantvik, FinlandIFF Health & Biosciences, 02460 Kantvik, FinlandIFF Health & Biosciences, 02460 Kantvik, FinlandIFF Health & Biosciences, 02460 Kantvik, FinlandIFF Health & Biosciences, Madison, WI 53716, USAIFF Health & Biosciences, Madison, WI 53716, USAIFF Health & Biosciences, 02460 Kantvik, FinlandIFF Health & Biosciences, 02460 Kantvik, FinlandIFF Health & Biosciences, 02460 Kantvik, FinlandOy 4Pharma Ltd., 20520 Turku, FinlandEstiMates Oy, 20520 Turku, FinlandIFF Health & Biosciences, 02460 Kantvik, FinlandCPS Research, Glasgow G20 7BE, UKIFF Health & Biosciences, 02460 Kantvik, FinlandCPS Research, Glasgow G20 7BE, UKIFF Health & Biosciences, 02460 Kantvik, FinlandThe primary objective of this randomised, placebo-controlled, triple-blind study was to assess whether orally consumed <i>Lactobacillus acidophilus</i> La-14 (La-14) and <i>Lacticaseibacillus rhamnosus</i> HN001 (HN001) colonise a healthy human vagina. Furthermore, potential effects on vaginal microbiota and immune markers were explored. Fifty women devoid of vaginal complaints (Nugent score 0–3 and vaginal pH ≤ 4.5) were randomised into a 2-week intervention with either La-14 and HN001 as the verum product or a comparable placebo. Vaginal swab samples were collected at baseline, after one and two weeks of intervention, and after a one-week follow-up, for assessing colonisation of the supplemented lactobacilli, vaginal microbiota, and six specific immune markers. Colonisation of <i>L. acidophilus</i> and <i>L. rhamnosus</i> was not observed above the assay detection limit (5.29 and 5.11 log 10 genomes/swab for <i>L. acidophilus</i> and <i>L. rhamnosus</i>, respectively). Vaginal microbiotas remained stable and predominated by lactobacilli throughout the intervention, and vaginal pH remained optimal (at least 90% of participants in both groups had pH 4.0 or 4.5 throughout the study). Immune markers elafin and human β-defensin 3 (HBD-3) were significantly decreased in the verum group (<i>p</i> = 0.022 and <i>p</i> = 0.028, respectively) but did not correlate with any microbiota changes. Adverse events raised no safety concerns, and no undesired changes in the vaginal microbiota or immune markers were detected.https://www.mdpi.com/2076-2607/11/2/499vaginal colonisationmicrobiotaimmune markerslactobacilliprobiotics<i>Lactobacillus acidophilus</i> |
| spellingShingle | Anna Lyra Reeta Ala-Jaakkola Nicolas Yeung Neeta Datta Kara Evans Ashley Hibberd Markus J. Lehtinen Sofia D. Forssten Alvin Ibarra Tommi Pesonen Jouni Junnila Arthur C. Ouwehand Keith Baranowski Johanna Maukonen Gordon Crawford Liisa Lehtoranta A Healthy Vaginal Microbiota Remains Stable during Oral Probiotic Supplementation: A Randomised Controlled Trial Microorganisms vaginal colonisation microbiota immune markers lactobacilli probiotics <i>Lactobacillus acidophilus</i> |
| title | A Healthy Vaginal Microbiota Remains Stable during Oral Probiotic Supplementation: A Randomised Controlled Trial |
| title_full | A Healthy Vaginal Microbiota Remains Stable during Oral Probiotic Supplementation: A Randomised Controlled Trial |
| title_fullStr | A Healthy Vaginal Microbiota Remains Stable during Oral Probiotic Supplementation: A Randomised Controlled Trial |
| title_full_unstemmed | A Healthy Vaginal Microbiota Remains Stable during Oral Probiotic Supplementation: A Randomised Controlled Trial |
| title_short | A Healthy Vaginal Microbiota Remains Stable during Oral Probiotic Supplementation: A Randomised Controlled Trial |
| title_sort | healthy vaginal microbiota remains stable during oral probiotic supplementation a randomised controlled trial |
| topic | vaginal colonisation microbiota immune markers lactobacilli probiotics <i>Lactobacillus acidophilus</i> |
| url | https://www.mdpi.com/2076-2607/11/2/499 |
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