Influence of the peritoneal lavage with bupivacaine on the survival and resistance of colonic anastomoses performed under fecal peritonitis in rats
PURPOSE: To evaluate the effects of peritoneal lavage with bupivacaine on survival and initial resistance of anastomosis on distal colon, performed under peritonitis or not. METHODS: Forty rats, weighing from 300 to 350g (321.29±11.31g), were randomly divided in four groups and underwent laparotomy...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2013-11-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/pdf/acb/v28n11/07.pdf |
| _version_ | 1818490630552158208 |
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| author | Michel Gardere Camargo João José Fagundes Raquel Franco Leal Maria de Lourdes Setsuko Ayrizono Débora Helena dos Gonçalves Rossi Priscilla de Sene Portel Oliveira Wu Feng Chung Huei Diana Lee Cláudio Saddy Rodrigues Coy |
| author_facet | Michel Gardere Camargo João José Fagundes Raquel Franco Leal Maria de Lourdes Setsuko Ayrizono Débora Helena dos Gonçalves Rossi Priscilla de Sene Portel Oliveira Wu Feng Chung Huei Diana Lee Cláudio Saddy Rodrigues Coy |
| author_sort | Michel Gardere Camargo |
| collection | DOAJ |
| description | PURPOSE: To evaluate the effects of peritoneal lavage with bupivacaine on survival and initial resistance of anastomosis on distal colon, performed under peritonitis or not. METHODS: Forty rats, weighing from 300 to 350g (321.29±11.31g), were randomly divided in four groups and underwent laparotomy and anastomosis on the distal colon six hours after induction of peritonitis by intraperitoneal injection of autologous fecal material or not. Group 1: No peritonitis and lavage with 3ml NS; Group 2: No peritonitis and lavage with 8 mg.kg-1 (± 0.5 mL) of 0.5% bupivacaine added to 2.5 mL of NS; Group 3: Peritonitis and lavage with 3ml NS; Group 4: Peritonitis and lavage with 8 mg.kg-1 (± 0.5 mL) of 0.5% bupivacaine added to 2.5 mL of NS. Necropsies were performed on the animals that died and the time of death was recorded. Surviving animals were submitted to euthanasia on the fifth post-operative day and Total Energy of Rupture biomechanical test (TER) was applied. RESULTS: Group 4 showed survival increase compared to Group 3, without statistical significance. Group 3 presented the smallest average TER, with statistical significance. CONCLUSION: Peritonitis increased mortality and influenced negatively the resistance of colic anastomosis in rats. Peritoneal lavage with bupivacaine increased anastomotic resistance. |
| first_indexed | 2024-12-10T17:19:33Z |
| format | Article |
| id | doaj.art-6f3e099e38ce48a5804a98f2cea025ce |
| institution | Directory Open Access Journal |
| issn | 0102-8650 |
| language | English |
| last_indexed | 2024-12-10T17:19:33Z |
| publishDate | 2013-11-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-6f3e099e38ce48a5804a98f2cea025ce2022-12-22T01:40:02ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira0102-86502013-11-01281178378710.1590/S0102-86502013001100007Influence of the peritoneal lavage with bupivacaine on the survival and resistance of colonic anastomoses performed under fecal peritonitis in ratsMichel Gardere Camargo0João José Fagundes1Raquel Franco Leal2Maria de Lourdes Setsuko Ayrizono3Débora Helena dos Gonçalves Rossi4Priscilla de Sene Portel Oliveira5Wu Feng Chung6Huei Diana Lee7Cláudio Saddy Rodrigues Coy8Universidade Estadual de CampinasUniversidade Estadual de CampinasUniversidade Estadual de CampinasUniversidade Estadual de CampinasUniversidade Estadual de CampinasUniversidade Estadual de CampinasUniversidade Estadual do Oeste do ParanáUniversidade Estadual do Oeste do ParanáUniversidade Estadual de CampinasPURPOSE: To evaluate the effects of peritoneal lavage with bupivacaine on survival and initial resistance of anastomosis on distal colon, performed under peritonitis or not. METHODS: Forty rats, weighing from 300 to 350g (321.29±11.31g), were randomly divided in four groups and underwent laparotomy and anastomosis on the distal colon six hours after induction of peritonitis by intraperitoneal injection of autologous fecal material or not. Group 1: No peritonitis and lavage with 3ml NS; Group 2: No peritonitis and lavage with 8 mg.kg-1 (± 0.5 mL) of 0.5% bupivacaine added to 2.5 mL of NS; Group 3: Peritonitis and lavage with 3ml NS; Group 4: Peritonitis and lavage with 8 mg.kg-1 (± 0.5 mL) of 0.5% bupivacaine added to 2.5 mL of NS. Necropsies were performed on the animals that died and the time of death was recorded. Surviving animals were submitted to euthanasia on the fifth post-operative day and Total Energy of Rupture biomechanical test (TER) was applied. RESULTS: Group 4 showed survival increase compared to Group 3, without statistical significance. Group 3 presented the smallest average TER, with statistical significance. CONCLUSION: Peritonitis increased mortality and influenced negatively the resistance of colic anastomosis in rats. Peritoneal lavage with bupivacaine increased anastomotic resistance.http://www.scielo.br/pdf/acb/v28n11/07.pdfAnesthetics, LocalBupivacaineAnastomosis, SurgicalColonPeritonitisRats |
| spellingShingle | Michel Gardere Camargo João José Fagundes Raquel Franco Leal Maria de Lourdes Setsuko Ayrizono Débora Helena dos Gonçalves Rossi Priscilla de Sene Portel Oliveira Wu Feng Chung Huei Diana Lee Cláudio Saddy Rodrigues Coy Influence of the peritoneal lavage with bupivacaine on the survival and resistance of colonic anastomoses performed under fecal peritonitis in rats Acta Cirúrgica Brasileira Anesthetics, Local Bupivacaine Anastomosis, Surgical Colon Peritonitis Rats |
| title | Influence of the peritoneal lavage with bupivacaine on the survival and resistance of colonic anastomoses performed under fecal peritonitis in rats |
| title_full | Influence of the peritoneal lavage with bupivacaine on the survival and resistance of colonic anastomoses performed under fecal peritonitis in rats |
| title_fullStr | Influence of the peritoneal lavage with bupivacaine on the survival and resistance of colonic anastomoses performed under fecal peritonitis in rats |
| title_full_unstemmed | Influence of the peritoneal lavage with bupivacaine on the survival and resistance of colonic anastomoses performed under fecal peritonitis in rats |
| title_short | Influence of the peritoneal lavage with bupivacaine on the survival and resistance of colonic anastomoses performed under fecal peritonitis in rats |
| title_sort | influence of the peritoneal lavage with bupivacaine on the survival and resistance of colonic anastomoses performed under fecal peritonitis in rats |
| topic | Anesthetics, Local Bupivacaine Anastomosis, Surgical Colon Peritonitis Rats |
| url | http://www.scielo.br/pdf/acb/v28n11/07.pdf |
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