Targeting Hepatic Protein Carbonylation and Oxidative Stress Occurring on Diet-Induced Metabolic Diseases through the Supplementation with Fish Oils
The present study addressed the ability of long-chain ω-3 polyunsaturated fatty acids (ω-3 PUFA), i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), to ameliorate liver protein damage derived from oxidative stress and induced by consumption of high-caloric diets, t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-09-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/16/10/353 |
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| author | Silvia Muñoz Lucía Méndez Gabriel Dasilva Josep Lluís Torres Sara Ramos-Romero Marta Romeu María Rosa Nogués Isabel Medina |
| author_facet | Silvia Muñoz Lucía Méndez Gabriel Dasilva Josep Lluís Torres Sara Ramos-Romero Marta Romeu María Rosa Nogués Isabel Medina |
| author_sort | Silvia Muñoz |
| collection | DOAJ |
| description | The present study addressed the ability of long-chain ω-3 polyunsaturated fatty acids (ω-3 PUFA), i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), to ameliorate liver protein damage derived from oxidative stress and induced by consumption of high-caloric diets, typical of Westernized countries. The experimental design included an animal model of Sprague-Dawley rats fed high-fat high-sucrose (HFHS) diet supplemented with ω-3 EPA and DHA for a complete hepatic proteome analysis to map carbonylated proteins involved in specific metabolic pathways. Results showed that the intake of marine ω-3 PUFA through diet significantly decreased liver protein carbonylation caused by long-term HFHS consumption and increased antioxidant system. Fish oil modulated the carbonylation level of more than twenty liver proteins involved in critical metabolic pathways, including lipid metabolism (e.g., albumin), carbohydrate metabolism (e.g., pyruvate carboxylase), detoxification process (e.g., aldehyde dehydrogenase 2), urea cycle (e.g., carbamoyl-phosphate synthase), cytoskeleton dynamics (e.g., actin), or response to oxidative stress (e.g., catalase) among others, which might be under the control of diet marine ω-3 PUFA. In parallel, fish oil significantly changed the liver fatty acid profile given by the HFHS diet, resulting in a more anti-inflammatory phenotype. In conclusion, the present study highlights the significance of marine ω-3 PUFA intake for the health of rats fed a Westernized diet by describing several key metabolic pathways which are protected in liver. |
| first_indexed | 2024-04-11T18:42:07Z |
| format | Article |
| id | doaj.art-6f3eaedb4a7c4acfa145b3078f7fbca7 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T18:42:07Z |
| publishDate | 2018-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-6f3eaedb4a7c4acfa145b3078f7fbca72022-12-22T04:08:58ZengMDPI AGMarine Drugs1660-33972018-09-01161035310.3390/md16100353md16100353Targeting Hepatic Protein Carbonylation and Oxidative Stress Occurring on Diet-Induced Metabolic Diseases through the Supplementation with Fish OilsSilvia Muñoz0Lucía Méndez1Gabriel Dasilva2Josep Lluís Torres3Sara Ramos-Romero4Marta Romeu5María Rosa Nogués6Isabel Medina7Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (IIM-CSIC), E-36208 Vigo, SpainInstituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (IIM-CSIC), E-36208 Vigo, SpainInstituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (IIM-CSIC), E-36208 Vigo, SpainInstituto de Química Avanzada de Catalunya, Consejo Superior de Investigaciones Científicas (IQAC-CSIC) Jordi Girona 18-26, E-08034 Barcelona, SpainInstituto de Química Avanzada de Catalunya, Consejo Superior de Investigaciones Científicas (IQAC-CSIC) Jordi Girona 18-26, E-08034 Barcelona, SpainUnidad de Farmacología, Facultad de Medicina, Universidad Rovira i Virgili, Sant Llorenç 21, E-43201 Reus, SpainUnidad de Farmacología, Facultad de Medicina, Universidad Rovira i Virgili, Sant Llorenç 21, E-43201 Reus, SpainInstituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (IIM-CSIC), E-36208 Vigo, SpainThe present study addressed the ability of long-chain ω-3 polyunsaturated fatty acids (ω-3 PUFA), i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), to ameliorate liver protein damage derived from oxidative stress and induced by consumption of high-caloric diets, typical of Westernized countries. The experimental design included an animal model of Sprague-Dawley rats fed high-fat high-sucrose (HFHS) diet supplemented with ω-3 EPA and DHA for a complete hepatic proteome analysis to map carbonylated proteins involved in specific metabolic pathways. Results showed that the intake of marine ω-3 PUFA through diet significantly decreased liver protein carbonylation caused by long-term HFHS consumption and increased antioxidant system. Fish oil modulated the carbonylation level of more than twenty liver proteins involved in critical metabolic pathways, including lipid metabolism (e.g., albumin), carbohydrate metabolism (e.g., pyruvate carboxylase), detoxification process (e.g., aldehyde dehydrogenase 2), urea cycle (e.g., carbamoyl-phosphate synthase), cytoskeleton dynamics (e.g., actin), or response to oxidative stress (e.g., catalase) among others, which might be under the control of diet marine ω-3 PUFA. In parallel, fish oil significantly changed the liver fatty acid profile given by the HFHS diet, resulting in a more anti-inflammatory phenotype. In conclusion, the present study highlights the significance of marine ω-3 PUFA intake for the health of rats fed a Westernized diet by describing several key metabolic pathways which are protected in liver.http://www.mdpi.com/1660-3397/16/10/353oxidative stresshigh-fat high-sucrose dietliver protein damagemarine omega-3 fatty acidscarbonylationfish oilsSprague-Dawley rat |
| spellingShingle | Silvia Muñoz Lucía Méndez Gabriel Dasilva Josep Lluís Torres Sara Ramos-Romero Marta Romeu María Rosa Nogués Isabel Medina Targeting Hepatic Protein Carbonylation and Oxidative Stress Occurring on Diet-Induced Metabolic Diseases through the Supplementation with Fish Oils Marine Drugs oxidative stress high-fat high-sucrose diet liver protein damage marine omega-3 fatty acids carbonylation fish oils Sprague-Dawley rat |
| title | Targeting Hepatic Protein Carbonylation and Oxidative Stress Occurring on Diet-Induced Metabolic Diseases through the Supplementation with Fish Oils |
| title_full | Targeting Hepatic Protein Carbonylation and Oxidative Stress Occurring on Diet-Induced Metabolic Diseases through the Supplementation with Fish Oils |
| title_fullStr | Targeting Hepatic Protein Carbonylation and Oxidative Stress Occurring on Diet-Induced Metabolic Diseases through the Supplementation with Fish Oils |
| title_full_unstemmed | Targeting Hepatic Protein Carbonylation and Oxidative Stress Occurring on Diet-Induced Metabolic Diseases through the Supplementation with Fish Oils |
| title_short | Targeting Hepatic Protein Carbonylation and Oxidative Stress Occurring on Diet-Induced Metabolic Diseases through the Supplementation with Fish Oils |
| title_sort | targeting hepatic protein carbonylation and oxidative stress occurring on diet induced metabolic diseases through the supplementation with fish oils |
| topic | oxidative stress high-fat high-sucrose diet liver protein damage marine omega-3 fatty acids carbonylation fish oils Sprague-Dawley rat |
| url | http://www.mdpi.com/1660-3397/16/10/353 |
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