Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types
IntroductionGastrointestinal illnesses associated with the consumption of shellfish contaminated with Vibrio parahaemolyticus have a negative impact on the shellfish industry due to recalls and loss of consumer confidence in products. This bacterial pathogen is very diverse and specific sequence typ...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1041942/full |
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| author | Kari A. Brossard Stoos Kari A. Brossard Stoos Jennifer Ren Robin R. Shields-Cutler Kelly L. Sams Shannon Caldwell Marvin B. Ho Gregg Rivara Cheryl A. Whistler Cheryl A. Whistler Stephen H. Jones Stephen H. Jones Martin Wiedmann Jamie DeMent Rodman G. Getchell Hélène Marquis |
| author_facet | Kari A. Brossard Stoos Kari A. Brossard Stoos Jennifer Ren Robin R. Shields-Cutler Kelly L. Sams Shannon Caldwell Marvin B. Ho Gregg Rivara Cheryl A. Whistler Cheryl A. Whistler Stephen H. Jones Stephen H. Jones Martin Wiedmann Jamie DeMent Rodman G. Getchell Hélène Marquis |
| author_sort | Kari A. Brossard Stoos |
| collection | DOAJ |
| description | IntroductionGastrointestinal illnesses associated with the consumption of shellfish contaminated with Vibrio parahaemolyticus have a negative impact on the shellfish industry due to recalls and loss of consumer confidence in products. This bacterial pathogen is very diverse and specific sequence types (STs), ST631 and ST36, have emerged as prevalent causes of Vibrio foodborne disease outbreaks in the US, though other STs have been implicated in sporadic cases. We investigated whether bacteriophages could be used as a proxy to monitor for the presence of distinct V. parahaemolyticus STs in coastal waters.MethodsFor this purpose, bacteriophages infecting V. parahaemolyticus were isolated from water samples collected on the Northeast Atlantic coast. The isolated phages were tested against a collection of 29 V. parahaemolyticus isolates representing 18 STs, including six clonal complexes (CC). Four distinct phages were identified based on their ability to infect different sets of V. parahaemolyticus isolates.Results and DiscussionOverall, the 29 bacterial isolates segregated into one of eight patterns of susceptibility, ranging from resistance to all four phages to susceptibility to any number of phages. STs represented by more than one bacterial isolate segregated within the same pattern of susceptibility except for one V. parahaemolyticus ST. Other patterns of susceptibility included exclusively clinical isolates represented by distinct STs. Overall, this study suggests that phages populating coastal waters could be exploited to monitor for the presence of V. parahaemolyticus STs known to cause foodborne outbreaks. |
| first_indexed | 2024-04-12T01:00:06Z |
| format | Article |
| id | doaj.art-6f408400d36146c7a7fd97385a1cb164 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T01:00:06Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6f408400d36146c7a7fd97385a1cb1642022-12-22T03:54:30ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-12-011310.3389/fmicb.2022.10419421041942Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence typesKari A. Brossard Stoos0Kari A. Brossard Stoos1Jennifer Ren2Robin R. Shields-Cutler3Kelly L. Sams4Shannon Caldwell5Marvin B. Ho6Gregg Rivara7Cheryl A. Whistler8Cheryl A. Whistler9Stephen H. Jones10Stephen H. Jones11Martin Wiedmann12Jamie DeMent13Rodman G. Getchell14Hélène Marquis15Department of Microbiology and Immunology, Cornell University, Ithaca, NY, United StatesDepartment of Health Promotion and Physical Education, Ithaca College, Ithaca, NY, United StatesDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY, United StatesDepartment of Biology, Macalester College, Saint Paul, MN, United StatesDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY, United StatesDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY, United StatesDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY, United StatesCornell Cooperative Extension of Suffolk County, Southold, NY, United StatesNortheast Center for Vibrio Disease and Ecology, University of New Hampshire, Durham, NH, United StatesDepartment of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, United StatesNortheast Center for Vibrio Disease and Ecology, University of New Hampshire, Durham, NH, United StatesDepartment of Natural Resources and the Environment, University of New Hampshire, Durham, NH, United StatesDepartment of Food Science, Cornell University, Ithaca, NY, United StatesFlorida Department of Health, Tallahassee, FL, United StatesDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY, United StatesDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY, United StatesIntroductionGastrointestinal illnesses associated with the consumption of shellfish contaminated with Vibrio parahaemolyticus have a negative impact on the shellfish industry due to recalls and loss of consumer confidence in products. This bacterial pathogen is very diverse and specific sequence types (STs), ST631 and ST36, have emerged as prevalent causes of Vibrio foodborne disease outbreaks in the US, though other STs have been implicated in sporadic cases. We investigated whether bacteriophages could be used as a proxy to monitor for the presence of distinct V. parahaemolyticus STs in coastal waters.MethodsFor this purpose, bacteriophages infecting V. parahaemolyticus were isolated from water samples collected on the Northeast Atlantic coast. The isolated phages were tested against a collection of 29 V. parahaemolyticus isolates representing 18 STs, including six clonal complexes (CC). Four distinct phages were identified based on their ability to infect different sets of V. parahaemolyticus isolates.Results and DiscussionOverall, the 29 bacterial isolates segregated into one of eight patterns of susceptibility, ranging from resistance to all four phages to susceptibility to any number of phages. STs represented by more than one bacterial isolate segregated within the same pattern of susceptibility except for one V. parahaemolyticus ST. Other patterns of susceptibility included exclusively clinical isolates represented by distinct STs. Overall, this study suggests that phages populating coastal waters could be exploited to monitor for the presence of V. parahaemolyticus STs known to cause foodborne outbreaks.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1041942/fullVibrio parahaemolyticusvibriophagesequence typephageST36 |
| spellingShingle | Kari A. Brossard Stoos Kari A. Brossard Stoos Jennifer Ren Robin R. Shields-Cutler Kelly L. Sams Shannon Caldwell Marvin B. Ho Gregg Rivara Cheryl A. Whistler Cheryl A. Whistler Stephen H. Jones Stephen H. Jones Martin Wiedmann Jamie DeMent Rodman G. Getchell Hélène Marquis Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types Frontiers in Microbiology Vibrio parahaemolyticus vibriophage sequence type phage ST36 |
| title | Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types |
| title_full | Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types |
| title_fullStr | Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types |
| title_full_unstemmed | Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types |
| title_short | Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types |
| title_sort | coastal water bacteriophages infect various sets of vibrio parahaemolyticus sequence types |
| topic | Vibrio parahaemolyticus vibriophage sequence type phage ST36 |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1041942/full |
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