Serum exosome microRNA panel as a noninvasive biomarker for molecular diagnosis of fulminant myocarditis
Exosome-derived microRNAs (miRNAs) are potential diagnostic biomarkers. However, little is known about their effectiveness as diagnostic biomarkers of fulminant myocarditis (FM). This study aimed to explore serum exosomal miRNAs as potential biomarkers for FM diagnosis. Peripheral blood samples were...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | Molecular Therapy: Methods & Clinical Development |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2329050120302333 |
| _version_ | 1818870733162414080 |
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| author | Yingying Zhang Xueqin Li Deguo Wang Xiaogan Jiang Mengying Zhang Kun Lv |
| author_facet | Yingying Zhang Xueqin Li Deguo Wang Xiaogan Jiang Mengying Zhang Kun Lv |
| author_sort | Yingying Zhang |
| collection | DOAJ |
| description | Exosome-derived microRNAs (miRNAs) are potential diagnostic biomarkers. However, little is known about their effectiveness as diagnostic biomarkers of fulminant myocarditis (FM). This study aimed to explore serum exosomal miRNAs as potential biomarkers for FM diagnosis. Peripheral blood samples were collected from 99 patients with FM, 32 patients with nonfulminant myocarditis (NFM), and 105 healthy controls (HCs). The miRNA expression profiles of serum exosomes were determined using next-generation sequencing, and differentially expressed miRNAs were further analyzed by quantitative reverse transcriptase polymerase chain reaction. A logistic regression model was constructed using a training cohort (n = 120) and then validated using an independent cohort (n = 106). The area under the receiver operating characteristic curve was used to evaluate diagnostic accuracy. In FM patients, hsa-miR-30a, hsa-miR-192, hsa-miR-146a, hsa-miR-155, and hsa-miR-320a were validated as significantly and differentially expressed candidates that could serve as potential markers for diagnosing FM. In addition, the miRNA panel (hsa-miR-155 and hsa-miR-320a) from the multivariate logistic regression model demonstrated high accuracy in the diagnosis of FM and was able to distinguish FM from HCs and NFM. Moreover, the diagnostic value of the miRNA panel was greater than that of CRP and cTn alone or together. The miRNA panel provided the excellent diagnostic capability for FM. |
| first_indexed | 2024-12-19T12:11:43Z |
| format | Article |
| id | doaj.art-6f49220127794d4698f5c208d7ae8adc |
| institution | Directory Open Access Journal |
| issn | 2329-0501 |
| language | English |
| last_indexed | 2024-12-19T12:11:43Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Methods & Clinical Development |
| spelling | doaj.art-6f49220127794d4698f5c208d7ae8adc2022-12-21T20:22:10ZengElsevierMolecular Therapy: Methods & Clinical Development2329-05012021-03-0120142151Serum exosome microRNA panel as a noninvasive biomarker for molecular diagnosis of fulminant myocarditisYingying Zhang0Xueqin Li1Deguo Wang2Xiaogan Jiang3Mengying Zhang4Kun Lv5Key Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institutes, Wuhu, PR China; Department of Laboratory Medicine, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu, PR ChinaKey Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institutes, Wuhu, PR China; Department of Central Laboratory, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, PR ChinaDepartment of Gerontology, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu, PR ChinaDepartment of Critical Care Medicine, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu, PR ChinaKey Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institutes, Wuhu, PR China; Department of Central Laboratory, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, PR ChinaKey Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institutes, Wuhu, PR China; Department of Central Laboratory, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, PR China; Corresponding author: Kun Lv, Department of Central Laboratory, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, 2 Zheshan Western Road, Wuhu 241001, China.Exosome-derived microRNAs (miRNAs) are potential diagnostic biomarkers. However, little is known about their effectiveness as diagnostic biomarkers of fulminant myocarditis (FM). This study aimed to explore serum exosomal miRNAs as potential biomarkers for FM diagnosis. Peripheral blood samples were collected from 99 patients with FM, 32 patients with nonfulminant myocarditis (NFM), and 105 healthy controls (HCs). The miRNA expression profiles of serum exosomes were determined using next-generation sequencing, and differentially expressed miRNAs were further analyzed by quantitative reverse transcriptase polymerase chain reaction. A logistic regression model was constructed using a training cohort (n = 120) and then validated using an independent cohort (n = 106). The area under the receiver operating characteristic curve was used to evaluate diagnostic accuracy. In FM patients, hsa-miR-30a, hsa-miR-192, hsa-miR-146a, hsa-miR-155, and hsa-miR-320a were validated as significantly and differentially expressed candidates that could serve as potential markers for diagnosing FM. In addition, the miRNA panel (hsa-miR-155 and hsa-miR-320a) from the multivariate logistic regression model demonstrated high accuracy in the diagnosis of FM and was able to distinguish FM from HCs and NFM. Moreover, the diagnostic value of the miRNA panel was greater than that of CRP and cTn alone or together. The miRNA panel provided the excellent diagnostic capability for FM.http://www.sciencedirect.com/science/article/pii/S2329050120302333exosomemicroRNA panelbiomarkermolecular diagnosisfulminant myocarditis |
| spellingShingle | Yingying Zhang Xueqin Li Deguo Wang Xiaogan Jiang Mengying Zhang Kun Lv Serum exosome microRNA panel as a noninvasive biomarker for molecular diagnosis of fulminant myocarditis Molecular Therapy: Methods & Clinical Development exosome microRNA panel biomarker molecular diagnosis fulminant myocarditis |
| title | Serum exosome microRNA panel as a noninvasive biomarker for molecular diagnosis of fulminant myocarditis |
| title_full | Serum exosome microRNA panel as a noninvasive biomarker for molecular diagnosis of fulminant myocarditis |
| title_fullStr | Serum exosome microRNA panel as a noninvasive biomarker for molecular diagnosis of fulminant myocarditis |
| title_full_unstemmed | Serum exosome microRNA panel as a noninvasive biomarker for molecular diagnosis of fulminant myocarditis |
| title_short | Serum exosome microRNA panel as a noninvasive biomarker for molecular diagnosis of fulminant myocarditis |
| title_sort | serum exosome microrna panel as a noninvasive biomarker for molecular diagnosis of fulminant myocarditis |
| topic | exosome microRNA panel biomarker molecular diagnosis fulminant myocarditis |
| url | http://www.sciencedirect.com/science/article/pii/S2329050120302333 |
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