HPLC-ESI-MS/MS of Imidazole Alkaloids in Pilocarpus microphyllus
Pilocarpine, an important imidazole alkaloid, is extracted from the leaves of Pilocarpus microphyllus (Rutaceae), known in Brazil as jaborandi and used mainly for the treatment of glaucoma. Jaborandi leaves also contain other imidazole alkaloids, whose pharmacological and physiological properties ar...
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| Format: | Article |
| Language: | English |
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MDPI AG
2008-07-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/13/7/1518/ |
| _version_ | 1819094324132970496 |
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| author | Paulo Mazzafera Marcos N. Eberlin Nathalia Luiza Andreazza Ilka Nacif Abreu Alexandra Sawaya |
| author_facet | Paulo Mazzafera Marcos N. Eberlin Nathalia Luiza Andreazza Ilka Nacif Abreu Alexandra Sawaya |
| author_sort | Paulo Mazzafera |
| collection | DOAJ |
| description | Pilocarpine, an important imidazole alkaloid, is extracted from the leaves of Pilocarpus microphyllus (Rutaceae), known in Brazil as jaborandi and used mainly for the treatment of glaucoma. Jaborandi leaves also contain other imidazole alkaloids, whose pharmacological and physiological properties are unknown, and whose biosynthetic pathways are under investigation. In the present study, a HPLC method coupled with ESI-MSn was developed for their qualitative and quantitative analysis. This method permits the chromatographic separation of the imidazole alkaloids found in extracts of jaborandi, as well as the MS/MS analysis of the individual compounds. Thus two samples: leaves of P. microphyllus and a paste that is left over after the industrial extraction of pilocarpine; were compared. The paste was found to contain significant amounts of pilocarpine and other imidazole alkaloids, but had a slightly different alkaloid profile than the leaf extract. The method is suitable for the routine analysis of samples containing these alkaloids, as well as for the separation and identification of known and novel alkaloids from this family, and may be applied to further studies of the biosynthetic pathway of pilocarpine in P. microphyllus. |
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| format | Article |
| id | doaj.art-6f4f75e138e1420296bd41c8f3422df5 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-21T23:25:36Z |
| publishDate | 2008-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6f4f75e138e1420296bd41c8f3422df52022-12-21T18:46:38ZengMDPI AGMolecules1420-30492008-07-011371518152910.3390/molecules13071518HPLC-ESI-MS/MS of Imidazole Alkaloids in Pilocarpus microphyllusPaulo MazzaferaMarcos N. EberlinNathalia Luiza AndreazzaIlka Nacif AbreuAlexandra SawayaPilocarpine, an important imidazole alkaloid, is extracted from the leaves of Pilocarpus microphyllus (Rutaceae), known in Brazil as jaborandi and used mainly for the treatment of glaucoma. Jaborandi leaves also contain other imidazole alkaloids, whose pharmacological and physiological properties are unknown, and whose biosynthetic pathways are under investigation. In the present study, a HPLC method coupled with ESI-MSn was developed for their qualitative and quantitative analysis. This method permits the chromatographic separation of the imidazole alkaloids found in extracts of jaborandi, as well as the MS/MS analysis of the individual compounds. Thus two samples: leaves of P. microphyllus and a paste that is left over after the industrial extraction of pilocarpine; were compared. The paste was found to contain significant amounts of pilocarpine and other imidazole alkaloids, but had a slightly different alkaloid profile than the leaf extract. The method is suitable for the routine analysis of samples containing these alkaloids, as well as for the separation and identification of known and novel alkaloids from this family, and may be applied to further studies of the biosynthetic pathway of pilocarpine in P. microphyllus.http://www.mdpi.com/1420-3049/13/7/1518/JaborandiPilocarpus microphyllusimidazole alkaloidspilocarpineHPLCESI- MS/MS. |
| spellingShingle | Paulo Mazzafera Marcos N. Eberlin Nathalia Luiza Andreazza Ilka Nacif Abreu Alexandra Sawaya HPLC-ESI-MS/MS of Imidazole Alkaloids in Pilocarpus microphyllus Molecules Jaborandi Pilocarpus microphyllus imidazole alkaloids pilocarpine HPLCESI- MS/MS. |
| title | HPLC-ESI-MS/MS of Imidazole Alkaloids in Pilocarpus microphyllus |
| title_full | HPLC-ESI-MS/MS of Imidazole Alkaloids in Pilocarpus microphyllus |
| title_fullStr | HPLC-ESI-MS/MS of Imidazole Alkaloids in Pilocarpus microphyllus |
| title_full_unstemmed | HPLC-ESI-MS/MS of Imidazole Alkaloids in Pilocarpus microphyllus |
| title_short | HPLC-ESI-MS/MS of Imidazole Alkaloids in Pilocarpus microphyllus |
| title_sort | hplc esi ms ms of imidazole alkaloids in pilocarpus microphyllus |
| topic | Jaborandi Pilocarpus microphyllus imidazole alkaloids pilocarpine HPLCESI- MS/MS. |
| url | http://www.mdpi.com/1420-3049/13/7/1518/ |
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