Liver tissue remodeling following ablation with irreversible electroporation in a porcine model
Irreversible electroporation (IRE) is a method of non-thermal focal tissue ablation characterized by irreversibly permeabilizing the cell membranes while preserving the extracellular matrix. This study aimed to investigate tissue remodeling after IRE in a porcine model, especially focusing on the ex...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.1014648/full |
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| author | Eva Monleón Eva Monleón Óscar Lucía Antonio Güemes Antonio Güemes Antonio Güemes Borja López-Alonso Dolores Arribas Dolores Arribas Dolores Arribas Héctor Sarnago Alba Hernaez Alba Hernaez José Miguel Burdío Concepción Junquera Concepción Junquera |
| author_facet | Eva Monleón Eva Monleón Óscar Lucía Antonio Güemes Antonio Güemes Antonio Güemes Borja López-Alonso Dolores Arribas Dolores Arribas Dolores Arribas Héctor Sarnago Alba Hernaez Alba Hernaez José Miguel Burdío Concepción Junquera Concepción Junquera |
| author_sort | Eva Monleón |
| collection | DOAJ |
| description | Irreversible electroporation (IRE) is a method of non-thermal focal tissue ablation characterized by irreversibly permeabilizing the cell membranes while preserving the extracellular matrix. This study aimed to investigate tissue remodeling after IRE in a porcine model, especially focusing on the extracellular matrix and hepatic stellate cells. IRE ablation was performed on 11 female pigs at 2,000 V/cm electric field strength using a versatile high-voltage generator and 3 cm diameter parallel-plate electrodes. The treated lobes were removed during surgery at 1, 3, 7, 14, and 21 days after IRE. Tissue remodeling and regeneration were assessed by histopathology and immunohistochemistry. Throughout the treated area, IRE led to extensive necrosis with intact collagenous structures evident until day 1. From then on, the necrosis progressively diminished while reparative tissue gradually increased. During this process, the reticulin framework and the septal fibrillar collagen remained in the necrotic foci until they were invaded by the reparative tissue. The reparative tissue was characterized by a massive proliferation of myofibroblast-like cells accompanied by a complete disorganization of the extracellular matrix with the disappearance of hepatic architecture. Hepatic stellate cell markers were associated with the proliferation of myofibroblast-like cells and the reorganization of the extracellular matrix. Between 2 and 3 weeks after IRE, the lobular architecture was almost completely regenerated. The events described in the present study show that IRE may be a valid model to study the mechanisms underlying liver regeneration after extensive acute injury. |
| first_indexed | 2024-04-12T14:20:49Z |
| format | Article |
| id | doaj.art-6f52728f9918456abdcbaaef3077428d |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-12T14:20:49Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-6f52728f9918456abdcbaaef3077428d2022-12-22T03:29:35ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-11-01910.3389/fvets.2022.10146481014648Liver tissue remodeling following ablation with irreversible electroporation in a porcine modelEva Monleón0Eva Monleón1Óscar Lucía2Antonio Güemes3Antonio Güemes4Antonio Güemes5Borja López-Alonso6Dolores Arribas7Dolores Arribas8Dolores Arribas9Héctor Sarnago10Alba Hernaez11Alba Hernaez12José Miguel Burdío13Concepción Junquera14Concepción Junquera15Department of Human Anatomy and Histology, University of Zaragoza, Zaragoza, SpainInstitute for Health Research Aragón (IIS), Zaragoza, SpainDepartment of Electronic Engineering and Communications, University of Zaragoza, Zaragoza, SpainInstitute for Health Research Aragón (IIS), Zaragoza, SpainDepartment of Surgery, University of Zaragoza, Zaragoza, SpainDepartment of General Surgery, Hospital Clínico Universitario Lozano Blesa, Zaragoza, SpainDepartment of Electronic Engineering and Communications, University of Zaragoza, Zaragoza, SpainInstitute for Health Research Aragón (IIS), Zaragoza, SpainDepartment of Surgery, University of Zaragoza, Zaragoza, SpainDepartment of General Surgery, Hospital Clínico Universitario Lozano Blesa, Zaragoza, SpainDepartment of Electronic Engineering and Communications, University of Zaragoza, Zaragoza, SpainInstitute for Health Research Aragón (IIS), Zaragoza, SpainDepartment of General Surgery, Hospital Clínico Universitario Lozano Blesa, Zaragoza, SpainDepartment of Electronic Engineering and Communications, University of Zaragoza, Zaragoza, SpainDepartment of Human Anatomy and Histology, University of Zaragoza, Zaragoza, SpainInstitute for Health Research Aragón (IIS), Zaragoza, SpainIrreversible electroporation (IRE) is a method of non-thermal focal tissue ablation characterized by irreversibly permeabilizing the cell membranes while preserving the extracellular matrix. This study aimed to investigate tissue remodeling after IRE in a porcine model, especially focusing on the extracellular matrix and hepatic stellate cells. IRE ablation was performed on 11 female pigs at 2,000 V/cm electric field strength using a versatile high-voltage generator and 3 cm diameter parallel-plate electrodes. The treated lobes were removed during surgery at 1, 3, 7, 14, and 21 days after IRE. Tissue remodeling and regeneration were assessed by histopathology and immunohistochemistry. Throughout the treated area, IRE led to extensive necrosis with intact collagenous structures evident until day 1. From then on, the necrosis progressively diminished while reparative tissue gradually increased. During this process, the reticulin framework and the septal fibrillar collagen remained in the necrotic foci until they were invaded by the reparative tissue. The reparative tissue was characterized by a massive proliferation of myofibroblast-like cells accompanied by a complete disorganization of the extracellular matrix with the disappearance of hepatic architecture. Hepatic stellate cell markers were associated with the proliferation of myofibroblast-like cells and the reorganization of the extracellular matrix. Between 2 and 3 weeks after IRE, the lobular architecture was almost completely regenerated. The events described in the present study show that IRE may be a valid model to study the mechanisms underlying liver regeneration after extensive acute injury.https://www.frontiersin.org/articles/10.3389/fvets.2022.1014648/fullirreversible electroporationliverhepatic stellate cellsextracellular matrixregeneration |
| spellingShingle | Eva Monleón Eva Monleón Óscar Lucía Antonio Güemes Antonio Güemes Antonio Güemes Borja López-Alonso Dolores Arribas Dolores Arribas Dolores Arribas Héctor Sarnago Alba Hernaez Alba Hernaez José Miguel Burdío Concepción Junquera Concepción Junquera Liver tissue remodeling following ablation with irreversible electroporation in a porcine model Frontiers in Veterinary Science irreversible electroporation liver hepatic stellate cells extracellular matrix regeneration |
| title | Liver tissue remodeling following ablation with irreversible electroporation in a porcine model |
| title_full | Liver tissue remodeling following ablation with irreversible electroporation in a porcine model |
| title_fullStr | Liver tissue remodeling following ablation with irreversible electroporation in a porcine model |
| title_full_unstemmed | Liver tissue remodeling following ablation with irreversible electroporation in a porcine model |
| title_short | Liver tissue remodeling following ablation with irreversible electroporation in a porcine model |
| title_sort | liver tissue remodeling following ablation with irreversible electroporation in a porcine model |
| topic | irreversible electroporation liver hepatic stellate cells extracellular matrix regeneration |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.1014648/full |
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