Identification of a Continuously Stable and Commercially Available Cell Line for the Identification of Infectious African Swine Fever Virus in Clinical Samples
African swine fever virus (ASFV) is causing outbreaks both in domestic pigs and wild boar in Europe and Asia. In 2018, the largest pig producing country, China, reported its first outbreak of African swine fever (ASF). Since then, the disease has quickly spread to all provinces in China and to other...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/8/820 |
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| author | Ayushi Rai Sarah Pruitt Elizabeth Ramirez-Medina Elizabeth A. Vuono Ediane Silva Lauro Velazquez-Salinas Consuelo Carrillo Manuel V. Borca Douglas P. Gladue |
| author_facet | Ayushi Rai Sarah Pruitt Elizabeth Ramirez-Medina Elizabeth A. Vuono Ediane Silva Lauro Velazquez-Salinas Consuelo Carrillo Manuel V. Borca Douglas P. Gladue |
| author_sort | Ayushi Rai |
| collection | DOAJ |
| description | African swine fever virus (ASFV) is causing outbreaks both in domestic pigs and wild boar in Europe and Asia. In 2018, the largest pig producing country, China, reported its first outbreak of African swine fever (ASF). Since then, the disease has quickly spread to all provinces in China and to other countries in southeast Asia, and most recently to India. Outbreaks of the disease occur in Europe as far west as Poland, and one isolated outbreak has been reported in Belgium. The current outbreak strain is highly contagious and can cause a high degree of lethality in domestic pigs, leading to widespread and costly losses to the industry. Currently, detection of infectious ASFV in field clinical samples requires accessibility to primary swine macrophage cultures, which are infrequently available in most regional veterinary diagnostic laboratories. Here, we report the identification of a commercially available cell line, MA-104, as a suitable substrate for virus isolation of African swine fever virus. |
| first_indexed | 2024-03-10T18:09:07Z |
| format | Article |
| id | doaj.art-6f52cf6b9abb4046a4a9ba08c42e0203 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T18:09:07Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-6f52cf6b9abb4046a4a9ba08c42e02032023-11-20T08:17:07ZengMDPI AGViruses1999-49152020-07-0112882010.3390/v12080820Identification of a Continuously Stable and Commercially Available Cell Line for the Identification of Infectious African Swine Fever Virus in Clinical SamplesAyushi Rai0Sarah Pruitt1Elizabeth Ramirez-Medina2Elizabeth A. Vuono3Ediane Silva4Lauro Velazquez-Salinas5Consuelo Carrillo6Manuel V. Borca7Douglas P. Gladue8ARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USAARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USAARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USAARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USAARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USAARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USAAPHIS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USAARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USAARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USAAfrican swine fever virus (ASFV) is causing outbreaks both in domestic pigs and wild boar in Europe and Asia. In 2018, the largest pig producing country, China, reported its first outbreak of African swine fever (ASF). Since then, the disease has quickly spread to all provinces in China and to other countries in southeast Asia, and most recently to India. Outbreaks of the disease occur in Europe as far west as Poland, and one isolated outbreak has been reported in Belgium. The current outbreak strain is highly contagious and can cause a high degree of lethality in domestic pigs, leading to widespread and costly losses to the industry. Currently, detection of infectious ASFV in field clinical samples requires accessibility to primary swine macrophage cultures, which are infrequently available in most regional veterinary diagnostic laboratories. Here, we report the identification of a commercially available cell line, MA-104, as a suitable substrate for virus isolation of African swine fever virus.https://www.mdpi.com/1999-4915/12/8/820ASFVASFdiagnostics |
| spellingShingle | Ayushi Rai Sarah Pruitt Elizabeth Ramirez-Medina Elizabeth A. Vuono Ediane Silva Lauro Velazquez-Salinas Consuelo Carrillo Manuel V. Borca Douglas P. Gladue Identification of a Continuously Stable and Commercially Available Cell Line for the Identification of Infectious African Swine Fever Virus in Clinical Samples Viruses ASFV ASF diagnostics |
| title | Identification of a Continuously Stable and Commercially Available Cell Line for the Identification of Infectious African Swine Fever Virus in Clinical Samples |
| title_full | Identification of a Continuously Stable and Commercially Available Cell Line for the Identification of Infectious African Swine Fever Virus in Clinical Samples |
| title_fullStr | Identification of a Continuously Stable and Commercially Available Cell Line for the Identification of Infectious African Swine Fever Virus in Clinical Samples |
| title_full_unstemmed | Identification of a Continuously Stable and Commercially Available Cell Line for the Identification of Infectious African Swine Fever Virus in Clinical Samples |
| title_short | Identification of a Continuously Stable and Commercially Available Cell Line for the Identification of Infectious African Swine Fever Virus in Clinical Samples |
| title_sort | identification of a continuously stable and commercially available cell line for the identification of infectious african swine fever virus in clinical samples |
| topic | ASFV ASF diagnostics |
| url | https://www.mdpi.com/1999-4915/12/8/820 |
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