A multiplexed microfluidic PCR assay for sensitive and specific point-of-care detection of Chlamydia trachomatis.
Chlamydia trachomatis (Ct) is the most common cause of bacterial sexually transmitted diseases (STD) worldwide. While commercial nucleic acid amplification tests (NAAT) are available for Ct, none are rapid or inexpensive enough to be used at the point-of-care (POC). Towards the first Ct POC NAAT, we...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3522697?pdf=render |
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| author | Deborah Dean Rosemary S Turingan Hans-Ulrich Thomann Anna Zolotova James Rothschild Sandeep J Joseph Timothy D Read Eugene Tan Richard F Selden |
| author_facet | Deborah Dean Rosemary S Turingan Hans-Ulrich Thomann Anna Zolotova James Rothschild Sandeep J Joseph Timothy D Read Eugene Tan Richard F Selden |
| author_sort | Deborah Dean |
| collection | DOAJ |
| description | Chlamydia trachomatis (Ct) is the most common cause of bacterial sexually transmitted diseases (STD) worldwide. While commercial nucleic acid amplification tests (NAAT) are available for Ct, none are rapid or inexpensive enough to be used at the point-of-care (POC). Towards the first Ct POC NAAT, we developed a microfluidic assay that simultaneously interrogates nine Ct loci in 20 minutes.Endocervical samples were selected from 263 women at high risk for Ct STDs (∼35% prevalence). A head-to-head comparison was performed with the Roche-Amplicor NAAT. 129 (49.0%) and 88 (33.5%) samples were positive by multiplex and Amplicor assays, respectively. Sequencing resolved 71 discrepant samples, confirming 53 of 53 positive multiplex samples and 12 of 18 positive Amplicor samples. The sensitivity and specificity were 91.5% and 100%, and 62.4% and 95.9%, respectively, for multiplex and Amplicor assays. Positive and negative predictive values were 100% and 91%, and 94.1% and 68.6%, respectively.This is the first rapid multiplex approach to Ct detection, and the assay was also found to be superior to a commercial NAAT. In effect, nine simultaneous reactions significantly increased sensitivity and specificity. Our assay can potentially increase Ct detection in globally diverse clinical settings at the POC. |
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| format | Article |
| id | doaj.art-6f530f25a070404ca7f2fe32e22d4776 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-24T01:01:02Z |
| publishDate | 2012-01-01 |
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| series | PLoS ONE |
| spelling | doaj.art-6f530f25a070404ca7f2fe32e22d47762022-12-21T17:23:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5168510.1371/journal.pone.0051685A multiplexed microfluidic PCR assay for sensitive and specific point-of-care detection of Chlamydia trachomatis.Deborah DeanRosemary S TuringanHans-Ulrich ThomannAnna ZolotovaJames RothschildSandeep J JosephTimothy D ReadEugene TanRichard F SeldenChlamydia trachomatis (Ct) is the most common cause of bacterial sexually transmitted diseases (STD) worldwide. While commercial nucleic acid amplification tests (NAAT) are available for Ct, none are rapid or inexpensive enough to be used at the point-of-care (POC). Towards the first Ct POC NAAT, we developed a microfluidic assay that simultaneously interrogates nine Ct loci in 20 minutes.Endocervical samples were selected from 263 women at high risk for Ct STDs (∼35% prevalence). A head-to-head comparison was performed with the Roche-Amplicor NAAT. 129 (49.0%) and 88 (33.5%) samples were positive by multiplex and Amplicor assays, respectively. Sequencing resolved 71 discrepant samples, confirming 53 of 53 positive multiplex samples and 12 of 18 positive Amplicor samples. The sensitivity and specificity were 91.5% and 100%, and 62.4% and 95.9%, respectively, for multiplex and Amplicor assays. Positive and negative predictive values were 100% and 91%, and 94.1% and 68.6%, respectively.This is the first rapid multiplex approach to Ct detection, and the assay was also found to be superior to a commercial NAAT. In effect, nine simultaneous reactions significantly increased sensitivity and specificity. Our assay can potentially increase Ct detection in globally diverse clinical settings at the POC.http://europepmc.org/articles/PMC3522697?pdf=render |
| spellingShingle | Deborah Dean Rosemary S Turingan Hans-Ulrich Thomann Anna Zolotova James Rothschild Sandeep J Joseph Timothy D Read Eugene Tan Richard F Selden A multiplexed microfluidic PCR assay for sensitive and specific point-of-care detection of Chlamydia trachomatis. PLoS ONE |
| title | A multiplexed microfluidic PCR assay for sensitive and specific point-of-care detection of Chlamydia trachomatis. |
| title_full | A multiplexed microfluidic PCR assay for sensitive and specific point-of-care detection of Chlamydia trachomatis. |
| title_fullStr | A multiplexed microfluidic PCR assay for sensitive and specific point-of-care detection of Chlamydia trachomatis. |
| title_full_unstemmed | A multiplexed microfluidic PCR assay for sensitive and specific point-of-care detection of Chlamydia trachomatis. |
| title_short | A multiplexed microfluidic PCR assay for sensitive and specific point-of-care detection of Chlamydia trachomatis. |
| title_sort | multiplexed microfluidic pcr assay for sensitive and specific point of care detection of chlamydia trachomatis |
| url | http://europepmc.org/articles/PMC3522697?pdf=render |
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