Graphene Based Electrochemical DNA Biosensor for Detection of False Smut of Rice ()
False smut caused by Ustilaginoidea virens is an important rice fungal disease that significantly decreases its production. In the recent past, conventional methods have been developed for its detection that is time-consuming and need high-cost equipments. The research and development in nanotechnol...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2021-06-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://www.ppjonline.org/upload/pdf/PPJ-OA-11-2020-0207.pdf |
| _version_ | 1811214598290800640 |
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| author | Kritika Rana Jagjiwan Mittal Jagriti Narang Annu Mishra Ramesh Namdeo Pudake |
| author_facet | Kritika Rana Jagjiwan Mittal Jagriti Narang Annu Mishra Ramesh Namdeo Pudake |
| author_sort | Kritika Rana |
| collection | DOAJ |
| description | False smut caused by Ustilaginoidea virens is an important rice fungal disease that significantly decreases its production. In the recent past, conventional methods have been developed for its detection that is time-consuming and need high-cost equipments. The research and development in nanotechnology have made it possible to assemble efficient recognition interfaces in biosensors. In this study, we present a simple, sensitive, and selective oxidized graphene-based geno-biosensor for the detection of rice false smut. The biosensor has been developed using a probe DNA as a biological recognition element on paper electrodes, and oxidized graphene to enhance the limit of detection and sensitivity of the sensor. Probe single-stranded DNA (ssDNA) and target ssDNA hybridization on the interface surface has been quantitatively measured with the electrochemical analysis tools namely, cyclic voltammetry, and linear sweep voltammetry. To confirm the selectivity of the device, probe hybridization with non-complementary ssDNA target has been studied. In our study, the developed sensor was able to detect up to 10 fM of target ssDNA. The paper electrodes were employed to produce an effective and cost-effective platform for the immobilization of the DNA and can be extended to design low-cost biosensors for the detection of the other plant pathogens. |
| first_indexed | 2024-04-12T06:07:17Z |
| format | Article |
| id | doaj.art-6f533ffd069a4df5adae64fc04dcef0b |
| institution | Directory Open Access Journal |
| issn | 1598-2254 2093-9280 |
| language | English |
| last_indexed | 2024-04-12T06:07:17Z |
| publishDate | 2021-06-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-6f533ffd069a4df5adae64fc04dcef0b2022-12-22T03:44:50ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802021-06-0137329129810.5423/PPJ.OA.11.2020.02072253Graphene Based Electrochemical DNA Biosensor for Detection of False Smut of Rice ()Kritika Rana0Jagjiwan Mittal1Jagriti Narang2Annu Mishra3Ramesh Namdeo Pudake4 Amity Institute of Nanotechnology, Amity University Uttar Pradesh, Noida 201303, UP, India Amity Institute of Nanotechnology, Amity University Uttar Pradesh, Noida 201303, UP, India Amity Institute of Nanotechnology, Amity University Uttar Pradesh, Noida 201303, UP, India Amity Institute of Nanotechnology, Amity University Uttar Pradesh, Noida 201303, UP, India Amity Institute of Nanotechnology, Amity University Uttar Pradesh, Noida 201303, UP, IndiaFalse smut caused by Ustilaginoidea virens is an important rice fungal disease that significantly decreases its production. In the recent past, conventional methods have been developed for its detection that is time-consuming and need high-cost equipments. The research and development in nanotechnology have made it possible to assemble efficient recognition interfaces in biosensors. In this study, we present a simple, sensitive, and selective oxidized graphene-based geno-biosensor for the detection of rice false smut. The biosensor has been developed using a probe DNA as a biological recognition element on paper electrodes, and oxidized graphene to enhance the limit of detection and sensitivity of the sensor. Probe single-stranded DNA (ssDNA) and target ssDNA hybridization on the interface surface has been quantitatively measured with the electrochemical analysis tools namely, cyclic voltammetry, and linear sweep voltammetry. To confirm the selectivity of the device, probe hybridization with non-complementary ssDNA target has been studied. In our study, the developed sensor was able to detect up to 10 fM of target ssDNA. The paper electrodes were employed to produce an effective and cost-effective platform for the immobilization of the DNA and can be extended to design low-cost biosensors for the detection of the other plant pathogens.http://www.ppjonline.org/upload/pdf/PPJ-OA-11-2020-0207.pdfdna probefalse smutgraphenenanosensorsplant pathogen electrochemical |
| spellingShingle | Kritika Rana Jagjiwan Mittal Jagriti Narang Annu Mishra Ramesh Namdeo Pudake Graphene Based Electrochemical DNA Biosensor for Detection of False Smut of Rice () The Plant Pathology Journal dna probe false smut graphene nanosensors plant pathogen electrochemical |
| title | Graphene Based Electrochemical DNA Biosensor for Detection of False Smut of Rice () |
| title_full | Graphene Based Electrochemical DNA Biosensor for Detection of False Smut of Rice () |
| title_fullStr | Graphene Based Electrochemical DNA Biosensor for Detection of False Smut of Rice () |
| title_full_unstemmed | Graphene Based Electrochemical DNA Biosensor for Detection of False Smut of Rice () |
| title_short | Graphene Based Electrochemical DNA Biosensor for Detection of False Smut of Rice () |
| title_sort | graphene based electrochemical dna biosensor for detection of false smut of rice |
| topic | dna probe false smut graphene nanosensors plant pathogen electrochemical |
| url | http://www.ppjonline.org/upload/pdf/PPJ-OA-11-2020-0207.pdf |
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