Stingless Bee Honey: Evaluating Its Antibacterial Activity and Bacterial Diversity
Stingless bee honey (SBH) is an astounding ‘miracle liquid’ with countless medicinal properties for various diseases such as gastroenteritis, cataracts, as well as for wound-healing. However, knowledge regarding it is still rather scarce. Henceforth, it is intriguing for us to contemplate on the les...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/8/500 |
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| author | Farah Nadiah Rosli Mohd Hafiz Fikri Hazemi Muhamad Afiq Akbar Syazwani Basir Hakimi Kassim Hamidun Bunawan |
| author_facet | Farah Nadiah Rosli Mohd Hafiz Fikri Hazemi Muhamad Afiq Akbar Syazwani Basir Hakimi Kassim Hamidun Bunawan |
| author_sort | Farah Nadiah Rosli |
| collection | DOAJ |
| description | Stingless bee honey (SBH) is an astounding ‘miracle liquid’ with countless medicinal properties for various diseases such as gastroenteritis, cataracts, as well as for wound-healing. However, knowledge regarding it is still rather scarce. Henceforth, it is intriguing for us to contemplate on the less-studied stingless bee and its honey in particular. First and foremost, the antimicrobial ability of honey from eight different stingless bee species was tested to further proven its health benefit. <i>Homotrigona fimbriata</i> honey showed the highest antimicrobial activity with inhibition against five bacteria; <i>Serratia marcescens</i>, <i>Escherichia coli</i>, <i>Bacillus subtilis</i>, <i>Alcaligenes faecalis</i> and <i>Staphylococcus aureus</i>. The next aim of our study is to characterize their honey bacterial community via the use of 16S rRNA amplicon sequencing technology. A total of eight bacterial phyla, 71 families, 155 genera and 70 species were identified from our study and two of the stingless bee species honey were determined to have the highest bacterial diversity compared to other six stingless bee species, namely <i>Heterotrigona erythrogastra</i> and <i>Tetrigona melanoleuca</i>. Furthermost, <i>Lactobacillus malefermentans</i> was thought to be the native dominant bacteria of SBH due to its predominant presence throughout all studied species. The aforementioned SBH’s antimicrobial results and characterization study of its bacterial diversity are hoped to carve the pathway towards extending its probiotic ability into our everyday lives. |
| first_indexed | 2024-03-10T17:59:19Z |
| format | Article |
| id | doaj.art-6f542e77b1eb43b2b528f7c09291c29b |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T17:59:19Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-6f542e77b1eb43b2b528f7c09291c29b2023-11-20T09:00:58ZengMDPI AGInsects2075-44502020-08-0111850010.3390/insects11080500Stingless Bee Honey: Evaluating Its Antibacterial Activity and Bacterial DiversityFarah Nadiah Rosli0Mohd Hafiz Fikri Hazemi1Muhamad Afiq Akbar2Syazwani Basir3Hakimi Kassim4Hamidun Bunawan5Centre for Biotechnology and Functional Food, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, MalaysiaInstitute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, MalaysiaCentre for Biotechnology and Functional Food, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, MalaysiaInstitute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, MalaysiaInstitute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, MalaysiaInstitute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, MalaysiaStingless bee honey (SBH) is an astounding ‘miracle liquid’ with countless medicinal properties for various diseases such as gastroenteritis, cataracts, as well as for wound-healing. However, knowledge regarding it is still rather scarce. Henceforth, it is intriguing for us to contemplate on the less-studied stingless bee and its honey in particular. First and foremost, the antimicrobial ability of honey from eight different stingless bee species was tested to further proven its health benefit. <i>Homotrigona fimbriata</i> honey showed the highest antimicrobial activity with inhibition against five bacteria; <i>Serratia marcescens</i>, <i>Escherichia coli</i>, <i>Bacillus subtilis</i>, <i>Alcaligenes faecalis</i> and <i>Staphylococcus aureus</i>. The next aim of our study is to characterize their honey bacterial community via the use of 16S rRNA amplicon sequencing technology. A total of eight bacterial phyla, 71 families, 155 genera and 70 species were identified from our study and two of the stingless bee species honey were determined to have the highest bacterial diversity compared to other six stingless bee species, namely <i>Heterotrigona erythrogastra</i> and <i>Tetrigona melanoleuca</i>. Furthermost, <i>Lactobacillus malefermentans</i> was thought to be the native dominant bacteria of SBH due to its predominant presence throughout all studied species. The aforementioned SBH’s antimicrobial results and characterization study of its bacterial diversity are hoped to carve the pathway towards extending its probiotic ability into our everyday lives.https://www.mdpi.com/2075-4450/11/8/500stingless beehoneyantimicrobialbacterial diversityamplicon sequencing |
| spellingShingle | Farah Nadiah Rosli Mohd Hafiz Fikri Hazemi Muhamad Afiq Akbar Syazwani Basir Hakimi Kassim Hamidun Bunawan Stingless Bee Honey: Evaluating Its Antibacterial Activity and Bacterial Diversity Insects stingless bee honey antimicrobial bacterial diversity amplicon sequencing |
| title | Stingless Bee Honey: Evaluating Its Antibacterial Activity and Bacterial Diversity |
| title_full | Stingless Bee Honey: Evaluating Its Antibacterial Activity and Bacterial Diversity |
| title_fullStr | Stingless Bee Honey: Evaluating Its Antibacterial Activity and Bacterial Diversity |
| title_full_unstemmed | Stingless Bee Honey: Evaluating Its Antibacterial Activity and Bacterial Diversity |
| title_short | Stingless Bee Honey: Evaluating Its Antibacterial Activity and Bacterial Diversity |
| title_sort | stingless bee honey evaluating its antibacterial activity and bacterial diversity |
| topic | stingless bee honey antimicrobial bacterial diversity amplicon sequencing |
| url | https://www.mdpi.com/2075-4450/11/8/500 |
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