Isolation, Characterization and Immunomodulatory Activity Evaluation of Chrysolaminarin from the Filamentous Microalga <i>Tribonema aequale</i>
The aim of this study is to investigate the differences in the accumulation capacity of chrysolaminarin among six <i>Tribonema</i> species and to isolate this polysaccharide for immunomodulatory activity evaluation. The results showed that <i>T. aequale</i> was the most produ...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/1/13 |
| _version_ | 1797439410101288960 |
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| author | Feifei Wang Rundong Yang Yuhao Guo Chengwu Zhang |
| author_facet | Feifei Wang Rundong Yang Yuhao Guo Chengwu Zhang |
| author_sort | Feifei Wang |
| collection | DOAJ |
| description | The aim of this study is to investigate the differences in the accumulation capacity of chrysolaminarin among six <i>Tribonema</i> species and to isolate this polysaccharide for immunomodulatory activity evaluation. The results showed that <i>T. aequale</i> was the most productive strain with the highest content and productivity of chrysolaminarin, which were 17.20% (% of dry weight) and 50.91 mg/L/d, respectively. Chrysolaminarin was then extracted and isolated from this alga, and its monosaccharide composition was mainly composed of a glucose (61.39%), linked by β-D-(1→3) (main chain) and β-D-(1→6) (branch chain) glycosidic bonds, with a molecular weight of less than 6 kDa. In vitro immunomodulatory assays showed that it could activate RAW264.7 cells at a certain concentration (1000 μg/mL), as evidenced by the increased phagocytic activity and upregulated mRNA expression levels of <i>IL-1β</i>, <i>IL6</i>, <i>TNF-α</i> and <i>Nos2</i>. Moreover, Western blot revealed that this polysaccharide stimulated the phosphorylation of p-65, p-38 and JNK in NF-κB and MAPK signaling pathways. Overall, these findings provide a reference for the further development and utilization of algae-based chrysolaminarin, while also offering an in-depth understanding of the immunoregulatory mechanism. |
| first_indexed | 2024-03-09T11:52:30Z |
| format | Article |
| id | doaj.art-6f5a8f21455948c799140be66d975ffe |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T11:52:30Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-6f5a8f21455948c799140be66d975ffe2023-11-30T23:13:17ZengMDPI AGMarine Drugs1660-33972022-12-012111310.3390/md21010013Isolation, Characterization and Immunomodulatory Activity Evaluation of Chrysolaminarin from the Filamentous Microalga <i>Tribonema aequale</i>Feifei Wang0Rundong Yang1Yuhao Guo2Chengwu Zhang3School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaSchool of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaSchool of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaDepartment of Ecology and Research Center for Hydrobiology, Jinan University, Guangzhou 510632, ChinaThe aim of this study is to investigate the differences in the accumulation capacity of chrysolaminarin among six <i>Tribonema</i> species and to isolate this polysaccharide for immunomodulatory activity evaluation. The results showed that <i>T. aequale</i> was the most productive strain with the highest content and productivity of chrysolaminarin, which were 17.20% (% of dry weight) and 50.91 mg/L/d, respectively. Chrysolaminarin was then extracted and isolated from this alga, and its monosaccharide composition was mainly composed of a glucose (61.39%), linked by β-D-(1→3) (main chain) and β-D-(1→6) (branch chain) glycosidic bonds, with a molecular weight of less than 6 kDa. In vitro immunomodulatory assays showed that it could activate RAW264.7 cells at a certain concentration (1000 μg/mL), as evidenced by the increased phagocytic activity and upregulated mRNA expression levels of <i>IL-1β</i>, <i>IL6</i>, <i>TNF-α</i> and <i>Nos2</i>. Moreover, Western blot revealed that this polysaccharide stimulated the phosphorylation of p-65, p-38 and JNK in NF-κB and MAPK signaling pathways. Overall, these findings provide a reference for the further development and utilization of algae-based chrysolaminarin, while also offering an in-depth understanding of the immunoregulatory mechanism.https://www.mdpi.com/1660-3397/21/1/13<i>Tribonema</i>chrysolaminarinisolationimmunomodulatory activity |
| spellingShingle | Feifei Wang Rundong Yang Yuhao Guo Chengwu Zhang Isolation, Characterization and Immunomodulatory Activity Evaluation of Chrysolaminarin from the Filamentous Microalga <i>Tribonema aequale</i> Marine Drugs <i>Tribonema</i> chrysolaminarin isolation immunomodulatory activity |
| title | Isolation, Characterization and Immunomodulatory Activity Evaluation of Chrysolaminarin from the Filamentous Microalga <i>Tribonema aequale</i> |
| title_full | Isolation, Characterization and Immunomodulatory Activity Evaluation of Chrysolaminarin from the Filamentous Microalga <i>Tribonema aequale</i> |
| title_fullStr | Isolation, Characterization and Immunomodulatory Activity Evaluation of Chrysolaminarin from the Filamentous Microalga <i>Tribonema aequale</i> |
| title_full_unstemmed | Isolation, Characterization and Immunomodulatory Activity Evaluation of Chrysolaminarin from the Filamentous Microalga <i>Tribonema aequale</i> |
| title_short | Isolation, Characterization and Immunomodulatory Activity Evaluation of Chrysolaminarin from the Filamentous Microalga <i>Tribonema aequale</i> |
| title_sort | isolation characterization and immunomodulatory activity evaluation of chrysolaminarin from the filamentous microalga i tribonema aequale i |
| topic | <i>Tribonema</i> chrysolaminarin isolation immunomodulatory activity |
| url | https://www.mdpi.com/1660-3397/21/1/13 |
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