Impacts of ZnO as a nanofertilizer on fenugreek: some biochemical parameters and SCoT analysis
Abstract Background Zinc oxide nanoparticles (ZnO NPs) can be considered as nanofertilizer providing zinc as an essential micronutrient for plant growth and production at specific safe dose, however, above this dose; ZnO NPs induce oxidative stress. The present research aimed to evaluate some physio...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-05-01
|
| Series: | Journal of Genetic Engineering and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43141-023-00501-0 |
| _version_ | 1827266958983168000 |
|---|---|
| author | Doaa E. Elsherif Eman Abd-ElShafy Asmaa M. Khalifa |
| author_facet | Doaa E. Elsherif Eman Abd-ElShafy Asmaa M. Khalifa |
| author_sort | Doaa E. Elsherif |
| collection | DOAJ |
| description | Abstract Background Zinc oxide nanoparticles (ZnO NPs) can be considered as nanofertilizer providing zinc as an essential micronutrient for plant growth and production at specific safe dose, however, above this dose; ZnO NPs induce oxidative stress. The present research aimed to evaluate some physiological and molecular effects of ZnO NPs on Trigonella foenum-graecum (fenugreek) plant. Results The ZnO NPs were applied at five different concentrations (10, 20, 30, 40, and 50 mg/l) via soaking fenugreek seeds for 24 h. Fenugreek seedlings were harvested after 14 days for biomass and biochemical analyses. The results revealed that increasing ZnO NPs concentration led to a significant increase in all measured parameters until peaked at 30 mg/l; after that, a decline trend was detected. However, malondialdehyde (MDA) increased significantly just at higher concentrations of ZnO NPs (40 and 50 mg/l). In addition, genetic variation measure using start codon targeted (SCoT) markers revealed that ZnO NP treatments exhibited limited genetic variation. Conclusion Results showed that treatment with ZnO NPs at 30 mg/l can improve biomass, bioactive compounds, and antioxidant activity of fenugreek seedlings, besides being safe for DNA. So, this concentration could be a decent nanofertilizer for fenugreek plant. |
| first_indexed | 2024-04-09T14:00:02Z |
| format | Article |
| id | doaj.art-6f5f6b12a5894c739d07311825edb501 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2025-03-22T04:26:07Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-6f5f6b12a5894c739d07311825edb5012024-04-28T05:52:40ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202023-05-0121111010.1186/s43141-023-00501-0Impacts of ZnO as a nanofertilizer on fenugreek: some biochemical parameters and SCoT analysisDoaa E. Elsherif0Eman Abd-ElShafy1Asmaa M. Khalifa2Botany Department, Faculty of Science, Tanta UniversityBotany and Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch)Botany and Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch)Abstract Background Zinc oxide nanoparticles (ZnO NPs) can be considered as nanofertilizer providing zinc as an essential micronutrient for plant growth and production at specific safe dose, however, above this dose; ZnO NPs induce oxidative stress. The present research aimed to evaluate some physiological and molecular effects of ZnO NPs on Trigonella foenum-graecum (fenugreek) plant. Results The ZnO NPs were applied at five different concentrations (10, 20, 30, 40, and 50 mg/l) via soaking fenugreek seeds for 24 h. Fenugreek seedlings were harvested after 14 days for biomass and biochemical analyses. The results revealed that increasing ZnO NPs concentration led to a significant increase in all measured parameters until peaked at 30 mg/l; after that, a decline trend was detected. However, malondialdehyde (MDA) increased significantly just at higher concentrations of ZnO NPs (40 and 50 mg/l). In addition, genetic variation measure using start codon targeted (SCoT) markers revealed that ZnO NP treatments exhibited limited genetic variation. Conclusion Results showed that treatment with ZnO NPs at 30 mg/l can improve biomass, bioactive compounds, and antioxidant activity of fenugreek seedlings, besides being safe for DNA. So, this concentration could be a decent nanofertilizer for fenugreek plant.https://doi.org/10.1186/s43141-023-00501-0FenugreekNanoparticlesMDADNASCoT analysis |
| spellingShingle | Doaa E. Elsherif Eman Abd-ElShafy Asmaa M. Khalifa Impacts of ZnO as a nanofertilizer on fenugreek: some biochemical parameters and SCoT analysis Journal of Genetic Engineering and Biotechnology Fenugreek Nanoparticles MDA DNA SCoT analysis |
| title | Impacts of ZnO as a nanofertilizer on fenugreek: some biochemical parameters and SCoT analysis |
| title_full | Impacts of ZnO as a nanofertilizer on fenugreek: some biochemical parameters and SCoT analysis |
| title_fullStr | Impacts of ZnO as a nanofertilizer on fenugreek: some biochemical parameters and SCoT analysis |
| title_full_unstemmed | Impacts of ZnO as a nanofertilizer on fenugreek: some biochemical parameters and SCoT analysis |
| title_short | Impacts of ZnO as a nanofertilizer on fenugreek: some biochemical parameters and SCoT analysis |
| title_sort | impacts of zno as a nanofertilizer on fenugreek some biochemical parameters and scot analysis |
| topic | Fenugreek Nanoparticles MDA DNA SCoT analysis |
| url | https://doi.org/10.1186/s43141-023-00501-0 |
| work_keys_str_mv | AT doaaeelsherif impactsofznoasananofertilizeronfenugreeksomebiochemicalparametersandscotanalysis AT emanabdelshafy impactsofznoasananofertilizeronfenugreeksomebiochemicalparametersandscotanalysis AT asmaamkhalifa impactsofznoasananofertilizeronfenugreeksomebiochemicalparametersandscotanalysis |