Oral administration of Faecalibacterium prausnitzii and Akkermansia muciniphila strains from humans improves atopic dermatitis symptoms in DNCB induced NC/Nga mice
Abstract Atopic dermatitis (AD) is a common inflammatory skin disease, and its pathogenesis is closely associated with microbial homeostasis in the gut, namely the gut-skin axis. Particularly, recent metagenomics studies revealed that the abundance of two major bacterial species in the gut, Faecalib...
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Nature Portfolio
2022-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-11048-4 |
| _version_ | 1818251128454774784 |
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| author | Yoonmi Lee Hye Rim Byeon Seo-Yul Jang Moon-Gi Hong Dohak Kim Dokyung Lee Joo-Hyun Shin Yesol Kim Seung-Goo Kang Jae-Gu Seo |
| author_facet | Yoonmi Lee Hye Rim Byeon Seo-Yul Jang Moon-Gi Hong Dohak Kim Dokyung Lee Joo-Hyun Shin Yesol Kim Seung-Goo Kang Jae-Gu Seo |
| author_sort | Yoonmi Lee |
| collection | DOAJ |
| description | Abstract Atopic dermatitis (AD) is a common inflammatory skin disease, and its pathogenesis is closely associated with microbial homeostasis in the gut, namely the gut-skin axis. Particularly, recent metagenomics studies revealed that the abundance of two major bacterial species in the gut, Faecalibacterium prausnitzii and Akkermansia muciniphila, may play a critical role in the pathogenesis of AD, but the effect of these species in AD has not yet been elucidated. To evaluate the potential beneficial effect of F. prausnitzii or A. muciniphila in AD, we conducted an animal model study where F. prausnitzii EB-FPDK11 or A. muciniphila EB-AMDK19, isolated from humans, was orally administered to 2,5-dinitrochlorobenzene (DNCB)-induced AD models using NC/Nga mice at a daily dose of 108 CFUs/mouse for six weeks. As a result, the administration of each strain of F. prausnitzii and A. muciniphila improved AD-related markers, such as dermatitis score, scratching behavior, and serum immunoglobulin E level. Also, the F. prausnitzii and A. muciniphila treatments decreased the level of thymic stromal lymphopoietin (TSLP), triggering the production of T helper (Th) 2 cytokines, and improved the imbalance between the Th1 and Th2 immune responses induced by DNCB. Meanwhile, the oral administration of the bacteria enhanced the production of filaggrin in the skin and ZO-1 in the gut barrier, leading to the recovery of functions. Taken together, our findings suggest that F. prausnitzii EB-FPDK11 and A. muciniphila EB-AMDK19 have a therapeutic potential in AD, which should be verified in humans. |
| first_indexed | 2024-12-12T16:03:21Z |
| format | Article |
| id | doaj.art-6f608827978f4cd38c2104510527a858 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T16:03:21Z |
| publishDate | 2022-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6f608827978f4cd38c2104510527a8582022-12-22T00:19:21ZengNature PortfolioScientific Reports2045-23222022-05-0112111510.1038/s41598-022-11048-4Oral administration of Faecalibacterium prausnitzii and Akkermansia muciniphila strains from humans improves atopic dermatitis symptoms in DNCB induced NC/Nga miceYoonmi Lee0Hye Rim Byeon1Seo-Yul Jang2Moon-Gi Hong3Dohak Kim4Dokyung Lee5Joo-Hyun Shin6Yesol Kim7Seung-Goo Kang8Jae-Gu Seo9R&D Center, Enterobiome Inc.R&D Center, Enterobiome Inc.R&D Center, Enterobiome Inc.R&D Center, Enterobiome Inc.R&D Center, Enterobiome Inc.R&D Center, Enterobiome Inc.R&D Center, Enterobiome Inc.Institute of Bioscience and Biotechnology, Kangwon National UniversityInstitute of Bioscience and Biotechnology, Kangwon National UniversityR&D Center, Enterobiome Inc.Abstract Atopic dermatitis (AD) is a common inflammatory skin disease, and its pathogenesis is closely associated with microbial homeostasis in the gut, namely the gut-skin axis. Particularly, recent metagenomics studies revealed that the abundance of two major bacterial species in the gut, Faecalibacterium prausnitzii and Akkermansia muciniphila, may play a critical role in the pathogenesis of AD, but the effect of these species in AD has not yet been elucidated. To evaluate the potential beneficial effect of F. prausnitzii or A. muciniphila in AD, we conducted an animal model study where F. prausnitzii EB-FPDK11 or A. muciniphila EB-AMDK19, isolated from humans, was orally administered to 2,5-dinitrochlorobenzene (DNCB)-induced AD models using NC/Nga mice at a daily dose of 108 CFUs/mouse for six weeks. As a result, the administration of each strain of F. prausnitzii and A. muciniphila improved AD-related markers, such as dermatitis score, scratching behavior, and serum immunoglobulin E level. Also, the F. prausnitzii and A. muciniphila treatments decreased the level of thymic stromal lymphopoietin (TSLP), triggering the production of T helper (Th) 2 cytokines, and improved the imbalance between the Th1 and Th2 immune responses induced by DNCB. Meanwhile, the oral administration of the bacteria enhanced the production of filaggrin in the skin and ZO-1 in the gut barrier, leading to the recovery of functions. Taken together, our findings suggest that F. prausnitzii EB-FPDK11 and A. muciniphila EB-AMDK19 have a therapeutic potential in AD, which should be verified in humans.https://doi.org/10.1038/s41598-022-11048-4 |
| spellingShingle | Yoonmi Lee Hye Rim Byeon Seo-Yul Jang Moon-Gi Hong Dohak Kim Dokyung Lee Joo-Hyun Shin Yesol Kim Seung-Goo Kang Jae-Gu Seo Oral administration of Faecalibacterium prausnitzii and Akkermansia muciniphila strains from humans improves atopic dermatitis symptoms in DNCB induced NC/Nga mice Scientific Reports |
| title | Oral administration of Faecalibacterium prausnitzii and Akkermansia muciniphila strains from humans improves atopic dermatitis symptoms in DNCB induced NC/Nga mice |
| title_full | Oral administration of Faecalibacterium prausnitzii and Akkermansia muciniphila strains from humans improves atopic dermatitis symptoms in DNCB induced NC/Nga mice |
| title_fullStr | Oral administration of Faecalibacterium prausnitzii and Akkermansia muciniphila strains from humans improves atopic dermatitis symptoms in DNCB induced NC/Nga mice |
| title_full_unstemmed | Oral administration of Faecalibacterium prausnitzii and Akkermansia muciniphila strains from humans improves atopic dermatitis symptoms in DNCB induced NC/Nga mice |
| title_short | Oral administration of Faecalibacterium prausnitzii and Akkermansia muciniphila strains from humans improves atopic dermatitis symptoms in DNCB induced NC/Nga mice |
| title_sort | oral administration of faecalibacterium prausnitzii and akkermansia muciniphila strains from humans improves atopic dermatitis symptoms in dncb induced nc nga mice |
| url | https://doi.org/10.1038/s41598-022-11048-4 |
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