Prevalence and Antifungal Susceptibility of Clinically Relevant <i>Candida</i> Species, Identification of <i>Candida auris</i> and <i>Kodamaea ohmeri</i> in Bangladesh
<i>Candida</i> species are major fungal pathogens in humans. The aim of this study was to determine the prevalence of individual <i>Candida</i> species and their susceptibility to antifungal drugs among clinical isolates in a tertiary care hospital in Bangladesh. During a 10-...
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MDPI AG
2022-08-01
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| Series: | Tropical Medicine and Infectious Disease |
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| Online Access: | https://www.mdpi.com/2414-6366/7/9/211 |
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| author | Fardousi Akter Sathi Shyamal Kumar Paul Salma Ahmed Mohammad Monirul Alam Syeda Anjuman Nasreen Nazia Haque Arup Islam Sultana Shabnam Nila Sultana Zahura Afrin Meiji Soe Aung Nobumichi Kobayashi |
| author_facet | Fardousi Akter Sathi Shyamal Kumar Paul Salma Ahmed Mohammad Monirul Alam Syeda Anjuman Nasreen Nazia Haque Arup Islam Sultana Shabnam Nila Sultana Zahura Afrin Meiji Soe Aung Nobumichi Kobayashi |
| author_sort | Fardousi Akter Sathi |
| collection | DOAJ |
| description | <i>Candida</i> species are major fungal pathogens in humans. The aim of this study was to determine the prevalence of individual <i>Candida</i> species and their susceptibility to antifungal drugs among clinical isolates in a tertiary care hospital in Bangladesh. During a 10-month period in 2021, high vaginal swabs (HVSs), blood, and aural swabs were collected from 360 patients. From these specimens, <i>Candida</i> spp. was isolated from cultures on Sabouraud dextrose agar media, and phenotypic and genetic analyses were performed. A total of 109 isolates were recovered, and <i>C. albicans</i> accounted for 37%, being derived mostly from HVSs. Among non-albicans <i>Candida</i> (NAC), <i>C. parapsilosis</i> was the most frequent, followed by <i>C. ciferrii</i>, <i>C. tropicalis</i>, and <i>C. glabrata</i>. Three isolates from blood and two isolates from aural discharge were genetically identified as <i>C. auris</i> and <i>Kodamaea ohmeri</i>, respectively. NAC isolates were more resistant to fluconazole (overall rate, 29%) than <i>C. albicans</i> (10%). <i>Candida</i> isolates from blood showed 95% susceptibility to voriconazole and less susceptibility to fluconazole (67%). Two or three amino acid substitutions were detected in the <i>ERG11</i> of two fluconazole-resistant <i>C. albicans</i> isolates. The present study is the first to reveal the prevalence of <i>Candida</i> species and their antifungal susceptibility in Bangladesh. |
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| institution | Directory Open Access Journal |
| issn | 2414-6366 |
| language | English |
| last_indexed | 2024-03-09T22:19:14Z |
| publishDate | 2022-08-01 |
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| spelling | doaj.art-6f67be2185914f3b8bf9102d040d62f82023-11-23T19:17:45ZengMDPI AGTropical Medicine and Infectious Disease2414-63662022-08-017921110.3390/tropicalmed7090211Prevalence and Antifungal Susceptibility of Clinically Relevant <i>Candida</i> Species, Identification of <i>Candida auris</i> and <i>Kodamaea ohmeri</i> in BangladeshFardousi Akter Sathi0Shyamal Kumar Paul1Salma Ahmed2Mohammad Monirul Alam3Syeda Anjuman Nasreen4Nazia Haque5Arup Islam6Sultana Shabnam Nila7Sultana Zahura Afrin8Meiji Soe Aung9Nobumichi Kobayashi10Department of Microbiology, Mymensingh Medical College, Mymensingh 2200, BangladeshNetrokona Medical College, Netrokona 2400, BangladeshDepartment of Microbiology, Mugda Medical College, Dhaka 1214, BangladeshDepartment of ENT, Mymensingh Medical College Hospital, Mymensingh 2200, BangladeshDepartment of Microbiology, Mymensingh Medical College, Mymensingh 2200, BangladeshDepartment of Microbiology, Mymensingh Medical College, Mymensingh 2200, BangladeshDepartment of Microbiology, Mymensingh Medical College, Mymensingh 2200, BangladeshDepartment of Microbiology, Mymensingh Medical College, Mymensingh 2200, BangladeshDepartment of Microbiology, Mymensingh Medical College, Mymensingh 2200, BangladeshDepartment of Hygiene, School of Medicine, Sapporo Medical University, Sapporo 060-8556, JapanDepartment of Hygiene, School of Medicine, Sapporo Medical University, Sapporo 060-8556, Japan<i>Candida</i> species are major fungal pathogens in humans. The aim of this study was to determine the prevalence of individual <i>Candida</i> species and their susceptibility to antifungal drugs among clinical isolates in a tertiary care hospital in Bangladesh. During a 10-month period in 2021, high vaginal swabs (HVSs), blood, and aural swabs were collected from 360 patients. From these specimens, <i>Candida</i> spp. was isolated from cultures on Sabouraud dextrose agar media, and phenotypic and genetic analyses were performed. A total of 109 isolates were recovered, and <i>C. albicans</i> accounted for 37%, being derived mostly from HVSs. Among non-albicans <i>Candida</i> (NAC), <i>C. parapsilosis</i> was the most frequent, followed by <i>C. ciferrii</i>, <i>C. tropicalis</i>, and <i>C. glabrata</i>. Three isolates from blood and two isolates from aural discharge were genetically identified as <i>C. auris</i> and <i>Kodamaea ohmeri</i>, respectively. NAC isolates were more resistant to fluconazole (overall rate, 29%) than <i>C. albicans</i> (10%). <i>Candida</i> isolates from blood showed 95% susceptibility to voriconazole and less susceptibility to fluconazole (67%). Two or three amino acid substitutions were detected in the <i>ERG11</i> of two fluconazole-resistant <i>C. albicans</i> isolates. The present study is the first to reveal the prevalence of <i>Candida</i> species and their antifungal susceptibility in Bangladesh.https://www.mdpi.com/2414-6366/7/9/211<i>Candida</i><i>C. albicans</i><i>C. auris</i><i>Kodamaea ohmeri</i>fluconazole resistance<i>ERG11</i> |
| spellingShingle | Fardousi Akter Sathi Shyamal Kumar Paul Salma Ahmed Mohammad Monirul Alam Syeda Anjuman Nasreen Nazia Haque Arup Islam Sultana Shabnam Nila Sultana Zahura Afrin Meiji Soe Aung Nobumichi Kobayashi Prevalence and Antifungal Susceptibility of Clinically Relevant <i>Candida</i> Species, Identification of <i>Candida auris</i> and <i>Kodamaea ohmeri</i> in Bangladesh Tropical Medicine and Infectious Disease <i>Candida</i> <i>C. albicans</i> <i>C. auris</i> <i>Kodamaea ohmeri</i> fluconazole resistance <i>ERG11</i> |
| title | Prevalence and Antifungal Susceptibility of Clinically Relevant <i>Candida</i> Species, Identification of <i>Candida auris</i> and <i>Kodamaea ohmeri</i> in Bangladesh |
| title_full | Prevalence and Antifungal Susceptibility of Clinically Relevant <i>Candida</i> Species, Identification of <i>Candida auris</i> and <i>Kodamaea ohmeri</i> in Bangladesh |
| title_fullStr | Prevalence and Antifungal Susceptibility of Clinically Relevant <i>Candida</i> Species, Identification of <i>Candida auris</i> and <i>Kodamaea ohmeri</i> in Bangladesh |
| title_full_unstemmed | Prevalence and Antifungal Susceptibility of Clinically Relevant <i>Candida</i> Species, Identification of <i>Candida auris</i> and <i>Kodamaea ohmeri</i> in Bangladesh |
| title_short | Prevalence and Antifungal Susceptibility of Clinically Relevant <i>Candida</i> Species, Identification of <i>Candida auris</i> and <i>Kodamaea ohmeri</i> in Bangladesh |
| title_sort | prevalence and antifungal susceptibility of clinically relevant i candida i species identification of i candida auris i and i kodamaea ohmeri i in bangladesh |
| topic | <i>Candida</i> <i>C. albicans</i> <i>C. auris</i> <i>Kodamaea ohmeri</i> fluconazole resistance <i>ERG11</i> |
| url | https://www.mdpi.com/2414-6366/7/9/211 |
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