Induction of Biosynthesis Antioxidant Molecules in Young Barley Plants by Trioxygen
Young barley plants are a good source of bioactive compounds. This paper presents the effects of gaseous O<sub>3</sub> (trioxygen or ozone) on the biosynthesis of compounds, determining the antioxidant potential of young barley plants. The total content of polyphenols was determined alon...
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MDPI AG
2022-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/21/7195 |
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| author | Natalia Matłok Tomasz Piechowiak Ireneusz Kapusta Kamil Królikowski Maciej Balawejder |
| author_facet | Natalia Matłok Tomasz Piechowiak Ireneusz Kapusta Kamil Królikowski Maciej Balawejder |
| author_sort | Natalia Matłok |
| collection | DOAJ |
| description | Young barley plants are a good source of bioactive compounds. This paper presents the effects of gaseous O<sub>3</sub> (trioxygen or ozone) on the biosynthesis of compounds, determining the antioxidant potential of young barley plants. The total content of polyphenols was determined along with their profile, as well as total antioxidant potential and vitamin C content. The highest contents of these compounds were identified in young barley plants exposed to gaseous O<sub>3</sub>. The main bioactive compound, representing polyphenols, determined in the examined raw materials was saponarin (isovitexin 7-<i>O</i>-glucoside). The induction of increased biosynthesis of these molecules was directly linked to the modification of the activity of selected enzymes. The increased polyphenol content resulted from the modified activities of polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL). On the other hand, the oxidative effect of ozone on barley plants was reduced, owing to the modified activities of catalases (CAT), glutathione peroxidases (SOD) and guaiacol peroxidase (GPOX). Analysis of the results showed that by applying gaseous O<sub>3</sub> at a dose of 50 ppm for 10 min, the contents of bioactive compounds can be maximised in a residue-free way by activating oxidative stress defence mechanisms. |
| first_indexed | 2024-03-09T18:48:57Z |
| format | Article |
| id | doaj.art-6f681e355878482dac92acf6c53cc07c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:48:57Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6f681e355878482dac92acf6c53cc07c2023-11-24T06:00:11ZengMDPI AGMolecules1420-30492022-10-012721719510.3390/molecules27217195Induction of Biosynthesis Antioxidant Molecules in Young Barley Plants by TrioxygenNatalia Matłok0Tomasz Piechowiak1Ireneusz Kapusta2Kamil Królikowski3Maciej Balawejder4Department of Food and Agriculture Production Engineering, University of Rzeszow, St. Zelwerowicza 4, 35-601 Rzeszow, PolandDepartment of Chemistry and Food Toxicology, University of Rzeszow, St. Ćwiklińskiej 1a, 35-601 Rzeszow, PolandDepartment of Food Technology and Human Nutrition, Rzeszow University, St. Zelwerowicza 4, 35-601 Rzeszow, PolandCollege of Natural Sciences, University of Rzeszow, St. Zelwerowicza 4, 35-601 Rzeszow, PolandDepartment of Chemistry and Food Toxicology, University of Rzeszow, St. Ćwiklińskiej 1a, 35-601 Rzeszow, PolandYoung barley plants are a good source of bioactive compounds. This paper presents the effects of gaseous O<sub>3</sub> (trioxygen or ozone) on the biosynthesis of compounds, determining the antioxidant potential of young barley plants. The total content of polyphenols was determined along with their profile, as well as total antioxidant potential and vitamin C content. The highest contents of these compounds were identified in young barley plants exposed to gaseous O<sub>3</sub>. The main bioactive compound, representing polyphenols, determined in the examined raw materials was saponarin (isovitexin 7-<i>O</i>-glucoside). The induction of increased biosynthesis of these molecules was directly linked to the modification of the activity of selected enzymes. The increased polyphenol content resulted from the modified activities of polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL). On the other hand, the oxidative effect of ozone on barley plants was reduced, owing to the modified activities of catalases (CAT), glutathione peroxidases (SOD) and guaiacol peroxidase (GPOX). Analysis of the results showed that by applying gaseous O<sub>3</sub> at a dose of 50 ppm for 10 min, the contents of bioactive compounds can be maximised in a residue-free way by activating oxidative stress defence mechanisms.https://www.mdpi.com/1420-3049/27/21/7195barley young leavesantioxidant activitiespolyphenolsUPLC–PDA–MS/MSsaponarin (isovitexin 7-<i>O</i>-glucoside)ROS generation |
| spellingShingle | Natalia Matłok Tomasz Piechowiak Ireneusz Kapusta Kamil Królikowski Maciej Balawejder Induction of Biosynthesis Antioxidant Molecules in Young Barley Plants by Trioxygen Molecules barley young leaves antioxidant activities polyphenols UPLC–PDA–MS/MS saponarin (isovitexin 7-<i>O</i>-glucoside) ROS generation |
| title | Induction of Biosynthesis Antioxidant Molecules in Young Barley Plants by Trioxygen |
| title_full | Induction of Biosynthesis Antioxidant Molecules in Young Barley Plants by Trioxygen |
| title_fullStr | Induction of Biosynthesis Antioxidant Molecules in Young Barley Plants by Trioxygen |
| title_full_unstemmed | Induction of Biosynthesis Antioxidant Molecules in Young Barley Plants by Trioxygen |
| title_short | Induction of Biosynthesis Antioxidant Molecules in Young Barley Plants by Trioxygen |
| title_sort | induction of biosynthesis antioxidant molecules in young barley plants by trioxygen |
| topic | barley young leaves antioxidant activities polyphenols UPLC–PDA–MS/MS saponarin (isovitexin 7-<i>O</i>-glucoside) ROS generation |
| url | https://www.mdpi.com/1420-3049/27/21/7195 |
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