Isolation of <i>Naegleria</i> spp. from a Brazilian Water Source
The genus <i>Naegleria</i>, of the free-living amoeba (FLA) group, has been investigated mainly due to its human health impact, resulting in deadly infections and their worldwide distribution on freshwater systems. <i>Naegleria fowleri</i>, colloquially known as the ̶...
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MDPI AG
2020-01-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/2/90 |
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| author | Natália Karla Bellini Ana Letícia Moreira da Fonseca María Reyes-Batlle Jacob Lorenzo-Morales Odete Rocha Otavio Henrique Thiemann |
| author_facet | Natália Karla Bellini Ana Letícia Moreira da Fonseca María Reyes-Batlle Jacob Lorenzo-Morales Odete Rocha Otavio Henrique Thiemann |
| author_sort | Natália Karla Bellini |
| collection | DOAJ |
| description | The genus <i>Naegleria</i>, of the free-living amoeba (FLA) group, has been investigated mainly due to its human health impact, resulting in deadly infections and their worldwide distribution on freshwater systems. <i>Naegleria fowleri</i>, colloquially known as the “brain-eating amoeba,” is the most studied <i>Naegleria</i> species because it causes primary amoebic meningoencephalitis (PAM) of high lethality. The assessment of FLA biodiversity is fundamental to evaluate the presence of pathogenic species and the possibility of human contamination. However, the knowledge of FLA distribution in Brazil is unknown, and to rectify this situation, we present research on identifying <i>Naegleria</i> spp. in the Monjolinho River as a model study. The river is a public Brazilian freshwater source that crosses the city of São Carlos, in São Paulo state, Brazil. Five distinct sampling sites were examined through limnological features, trophozoites culturing, and PCR against internal transcribed spacer (ITS) regions and 5.8S rRNA sequences. The results identified <i>N. philippinensis, N. canariensisi, N. australiensis, N. gruberi, N. dobsoni</i> sequences, as well as a <i>Hartmannella</i> sequence. The methodology delineated here represents the first Brazilian <i>Naegleria</i> spp. study on a freshwater system. Our results stress the urgency of a large scale evaluation of the presence of free-living amoebas in Brazil. |
| first_indexed | 2024-04-14T03:40:33Z |
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| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-04-14T03:40:33Z |
| publishDate | 2020-01-01 |
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| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-6f6e7812599743069dd26e7d0a1ba69b2022-12-22T02:14:32ZengMDPI AGPathogens2076-08172020-01-01929010.3390/pathogens9020090pathogens9020090Isolation of <i>Naegleria</i> spp. from a Brazilian Water SourceNatália Karla Bellini0Ana Letícia Moreira da Fonseca1María Reyes-Batlle2Jacob Lorenzo-Morales3Odete Rocha4Otavio Henrique Thiemann5Instituto de Física de São Carlos, Universidade de São Paulo, Caixa Postal 369, São Carlos, SP 13560-590, BrazilInstituto de Física de São Carlos, Universidade de São Paulo, Caixa Postal 369, São Carlos, SP 13560-590, BrazilInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203 La Laguna, Tenerife, Canary Islands, SpainInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203 La Laguna, Tenerife, Canary Islands, SpainDepartamento de Ecologia e Biologia Evolutiva, Universidade Federal de São Carlos, Rodovia Washington Luis, km 235, São Carlos, SP 13565-905, BrazilInstituto de Física de São Carlos, Universidade de São Paulo, Caixa Postal 369, São Carlos, SP 13560-590, BrazilThe genus <i>Naegleria</i>, of the free-living amoeba (FLA) group, has been investigated mainly due to its human health impact, resulting in deadly infections and their worldwide distribution on freshwater systems. <i>Naegleria fowleri</i>, colloquially known as the “brain-eating amoeba,” is the most studied <i>Naegleria</i> species because it causes primary amoebic meningoencephalitis (PAM) of high lethality. The assessment of FLA biodiversity is fundamental to evaluate the presence of pathogenic species and the possibility of human contamination. However, the knowledge of FLA distribution in Brazil is unknown, and to rectify this situation, we present research on identifying <i>Naegleria</i> spp. in the Monjolinho River as a model study. The river is a public Brazilian freshwater source that crosses the city of São Carlos, in São Paulo state, Brazil. Five distinct sampling sites were examined through limnological features, trophozoites culturing, and PCR against internal transcribed spacer (ITS) regions and 5.8S rRNA sequences. The results identified <i>N. philippinensis, N. canariensisi, N. australiensis, N. gruberi, N. dobsoni</i> sequences, as well as a <i>Hartmannella</i> sequence. The methodology delineated here represents the first Brazilian <i>Naegleria</i> spp. study on a freshwater system. Our results stress the urgency of a large scale evaluation of the presence of free-living amoebas in Brazil.https://www.mdpi.com/2076-0817/9/2/90<i>naegleria</i> spp.free-living amoebapcrmonjolinho riverbrazil |
| spellingShingle | Natália Karla Bellini Ana Letícia Moreira da Fonseca María Reyes-Batlle Jacob Lorenzo-Morales Odete Rocha Otavio Henrique Thiemann Isolation of <i>Naegleria</i> spp. from a Brazilian Water Source Pathogens <i>naegleria</i> spp. free-living amoeba pcr monjolinho river brazil |
| title | Isolation of <i>Naegleria</i> spp. from a Brazilian Water Source |
| title_full | Isolation of <i>Naegleria</i> spp. from a Brazilian Water Source |
| title_fullStr | Isolation of <i>Naegleria</i> spp. from a Brazilian Water Source |
| title_full_unstemmed | Isolation of <i>Naegleria</i> spp. from a Brazilian Water Source |
| title_short | Isolation of <i>Naegleria</i> spp. from a Brazilian Water Source |
| title_sort | isolation of i naegleria i spp from a brazilian water source |
| topic | <i>naegleria</i> spp. free-living amoeba pcr monjolinho river brazil |
| url | https://www.mdpi.com/2076-0817/9/2/90 |
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