Effects of Co-Inoculation of Indole-3-Acetic Acid-Producing and -Degrading Bacterial Endophytes on Plant Growth
Bacterial production of indole-3-acetic acid (IAA) and its effects on plant growth have been frequently studied but there have been few studies on the ecology of IAA-degrading bacteria. In this study, among eight endophytic bacterial strains previously isolated from the same sweet potato sample incl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-02-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/5/1/17 |
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| author | Sabitri Adhikari Dhungana Kazuhito Itoh |
| author_facet | Sabitri Adhikari Dhungana Kazuhito Itoh |
| author_sort | Sabitri Adhikari Dhungana |
| collection | DOAJ |
| description | Bacterial production of indole-3-acetic acid (IAA) and its effects on plant growth have been frequently studied but there have been few studies on the ecology of IAA-degrading bacteria. In this study, among eight endophytic bacterial strains previously isolated from the same sweet potato sample including two IAA producers, <i>Klebsiella</i> sp. Sal 1 and <i>Enterobacter</i> sp. Sal 3, all of the strains showed IAA-degrading ability to some extent. <i>Herbaspirillum</i> sp. Sal 6 had the highest activity for IAA and tryptophan. When the IAA producers and the degrader were co-cultured in tryptophan-amended N<sup>+</sup>MR liquid medium, the concentrations of IAA decreased. Inoculation with <i>Klebsiella</i> sp. Sal 1, the highest IAA producer among the test strains, increased fresh root weight of tomato and radish, but the effect decreased by co-inoculation with IAA-degrading <i>Herbaspirillum</i> sp. Sal 6. Since both strains colonized plant parts at high populations, it was likely that the IAA degrader decreased IAA levels in the plants by degrading IAA and/or its precursor tryptophan. When IAA-producing biofertilizers are used, interactions with IAA degraders in plants should be considered. |
| first_indexed | 2024-04-13T04:21:19Z |
| format | Article |
| id | doaj.art-6f74d85356d04133ac1e44c5e94bd6a1 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-04-13T04:21:19Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-6f74d85356d04133ac1e44c5e94bd6a12022-12-22T03:02:46ZengMDPI AGHorticulturae2311-75242019-02-01511710.3390/horticulturae5010017horticulturae5010017Effects of Co-Inoculation of Indole-3-Acetic Acid-Producing and -Degrading Bacterial Endophytes on Plant GrowthSabitri Adhikari Dhungana0Kazuhito Itoh1The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-minami, Tottori 680-8553, JapanThe United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-minami, Tottori 680-8553, JapanBacterial production of indole-3-acetic acid (IAA) and its effects on plant growth have been frequently studied but there have been few studies on the ecology of IAA-degrading bacteria. In this study, among eight endophytic bacterial strains previously isolated from the same sweet potato sample including two IAA producers, <i>Klebsiella</i> sp. Sal 1 and <i>Enterobacter</i> sp. Sal 3, all of the strains showed IAA-degrading ability to some extent. <i>Herbaspirillum</i> sp. Sal 6 had the highest activity for IAA and tryptophan. When the IAA producers and the degrader were co-cultured in tryptophan-amended N<sup>+</sup>MR liquid medium, the concentrations of IAA decreased. Inoculation with <i>Klebsiella</i> sp. Sal 1, the highest IAA producer among the test strains, increased fresh root weight of tomato and radish, but the effect decreased by co-inoculation with IAA-degrading <i>Herbaspirillum</i> sp. Sal 6. Since both strains colonized plant parts at high populations, it was likely that the IAA degrader decreased IAA levels in the plants by degrading IAA and/or its precursor tryptophan. When IAA-producing biofertilizers are used, interactions with IAA degraders in plants should be considered.https://www.mdpi.com/2311-7524/5/1/17endophyteindole-3-acetic acid (IAA)degradationco-cultureco-inoculationcolonization |
| spellingShingle | Sabitri Adhikari Dhungana Kazuhito Itoh Effects of Co-Inoculation of Indole-3-Acetic Acid-Producing and -Degrading Bacterial Endophytes on Plant Growth Horticulturae endophyte indole-3-acetic acid (IAA) degradation co-culture co-inoculation colonization |
| title | Effects of Co-Inoculation of Indole-3-Acetic Acid-Producing and -Degrading Bacterial Endophytes on Plant Growth |
| title_full | Effects of Co-Inoculation of Indole-3-Acetic Acid-Producing and -Degrading Bacterial Endophytes on Plant Growth |
| title_fullStr | Effects of Co-Inoculation of Indole-3-Acetic Acid-Producing and -Degrading Bacterial Endophytes on Plant Growth |
| title_full_unstemmed | Effects of Co-Inoculation of Indole-3-Acetic Acid-Producing and -Degrading Bacterial Endophytes on Plant Growth |
| title_short | Effects of Co-Inoculation of Indole-3-Acetic Acid-Producing and -Degrading Bacterial Endophytes on Plant Growth |
| title_sort | effects of co inoculation of indole 3 acetic acid producing and degrading bacterial endophytes on plant growth |
| topic | endophyte indole-3-acetic acid (IAA) degradation co-culture co-inoculation colonization |
| url | https://www.mdpi.com/2311-7524/5/1/17 |
| work_keys_str_mv | AT sabitriadhikaridhungana effectsofcoinoculationofindole3aceticacidproducinganddegradingbacterialendophytesonplantgrowth AT kazuhitoitoh effectsofcoinoculationofindole3aceticacidproducinganddegradingbacterialendophytesonplantgrowth |