Pathogenetic features of experimental osteoarthrosis induced by dexamethasone and talc
The aim of the study was to investigate the pathogenesis of experimental osteoarthrosis induced by dexamethasone and talc by examining the structure and defining the morphometric and metabolic features of knee joint skeletal connective tissues in rats.Materials and methods. We performed a morphometr...
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| Format: | Article |
| Language: | English |
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Siberian State Medical University (Tomsk)
2022-07-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/4807 |
| _version_ | 1797872763734589440 |
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| author | E. V. Gladkova |
| author_facet | E. V. Gladkova |
| author_sort | E. V. Gladkova |
| collection | DOAJ |
| description | The aim of the study was to investigate the pathogenesis of experimental osteoarthrosis induced by dexamethasone and talc by examining the structure and defining the morphometric and metabolic features of knee joint skeletal connective tissues in rats.Materials and methods. We performed a morphometric evaluation of articular cartilages (their thickness, extracellular matrix arrangement, spatial arrangement of the main components, distribution density, and main cellular indices of chondrocytes), as well as changes in subchondral bones (the presence of trabeculae in the basal layer of the articular cartilage and individual osteophytes) in 30 rats with a model of primary osteoarthrosis induced by sequential administration of 0.5 ml dexamethasone (2 mg) and 1 ml 10% sterile talc suspension mixed with normal saline into the joint cavity. We studied the histologic specimens of the knee joints stained with hematoxylin – eosin, Alcian blue (рН 1.0 and 2.5), as well as with Van Gieson’s, Masson’s, and Mallory’s trichome stains. The metabolic features of the articular cartilage and bone tissues were investigated by determining the hyaluronan, osteocalcin, and type I collagen levels in the serum of the rats.Results. In the rats with dexamethasone- and talc-induced osteoarthrosis, the thickness of cartilages in their weight-bearing areas decreased by 50%, the spatial arrangement of chondrocytes was impaired, and the nuclear – cytoplasmatic ratio (р < 0.01) decreased to 0.3. Besides, a rise in the serum levels of hyaluronan (p < 0.001) to 110.2 ng / ml, type I collagen fragments (p < 0.001) to 217.9 ng / ml, and osteocalcin (p < 0.001) to 231.1 ng / ml was detected.Conclusion. The main pathogenetic features of experimental osteoarthrosis induced by dexamethasone and talc include impaired distribution density, morphological characteristics, and functional activity of chondrocytes, which results in inhibited synthesis of extracellular matrix components in the articular cartilage and activated destruction of proteoglycans containing unsulphated glycosaminoglycans. The subchondral bone remodeling in experimental osteoarthrosis induced by dexamethasone and talc is characterized by intensification of synthetic activity of osteoblasts. |
| first_indexed | 2024-04-10T01:05:19Z |
| format | Article |
| id | doaj.art-6f75b0ffdb194fb3a5d87844bab4f744 |
| institution | Directory Open Access Journal |
| issn | 1682-0363 1819-3684 |
| language | English |
| last_indexed | 2024-04-10T01:05:19Z |
| publishDate | 2022-07-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj.art-6f75b0ffdb194fb3a5d87844bab4f7442023-03-13T09:58:28ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842022-07-01212192610.20538/1682-0363-2022-2-19-262885Pathogenetic features of experimental osteoarthrosis induced by dexamethasone and talcE. V. Gladkova0Научно-исследовательский институт травматологии, ортопедии и нейрохирургии (НИИТОН), Саратовский государственный медицинский университет (СГМУ) им. В.И. РазумовскогоThe aim of the study was to investigate the pathogenesis of experimental osteoarthrosis induced by dexamethasone and talc by examining the structure and defining the morphometric and metabolic features of knee joint skeletal connective tissues in rats.Materials and methods. We performed a morphometric evaluation of articular cartilages (their thickness, extracellular matrix arrangement, spatial arrangement of the main components, distribution density, and main cellular indices of chondrocytes), as well as changes in subchondral bones (the presence of trabeculae in the basal layer of the articular cartilage and individual osteophytes) in 30 rats with a model of primary osteoarthrosis induced by sequential administration of 0.5 ml dexamethasone (2 mg) and 1 ml 10% sterile talc suspension mixed with normal saline into the joint cavity. We studied the histologic specimens of the knee joints stained with hematoxylin – eosin, Alcian blue (рН 1.0 and 2.5), as well as with Van Gieson’s, Masson’s, and Mallory’s trichome stains. The metabolic features of the articular cartilage and bone tissues were investigated by determining the hyaluronan, osteocalcin, and type I collagen levels in the serum of the rats.Results. In the rats with dexamethasone- and talc-induced osteoarthrosis, the thickness of cartilages in their weight-bearing areas decreased by 50%, the spatial arrangement of chondrocytes was impaired, and the nuclear – cytoplasmatic ratio (р < 0.01) decreased to 0.3. Besides, a rise in the serum levels of hyaluronan (p < 0.001) to 110.2 ng / ml, type I collagen fragments (p < 0.001) to 217.9 ng / ml, and osteocalcin (p < 0.001) to 231.1 ng / ml was detected.Conclusion. The main pathogenetic features of experimental osteoarthrosis induced by dexamethasone and talc include impaired distribution density, morphological characteristics, and functional activity of chondrocytes, which results in inhibited synthesis of extracellular matrix components in the articular cartilage and activated destruction of proteoglycans containing unsulphated glycosaminoglycans. The subchondral bone remodeling in experimental osteoarthrosis induced by dexamethasone and talc is characterized by intensification of synthetic activity of osteoblasts.https://bulletin.ssmu.ru/jour/article/view/4807остеоартрозкрысыдексаметазонсуставной хрящхондроцитыостеокальцинсубхондральная костьгиалуроновая кислотаколлаген i типа |
| spellingShingle | E. V. Gladkova Pathogenetic features of experimental osteoarthrosis induced by dexamethasone and talc Бюллетень сибирской медицины остеоартроз крысы дексаметазон суставной хрящ хондроциты остеокальцин субхондральная кость гиалуроновая кислота коллаген i типа |
| title | Pathogenetic features of experimental osteoarthrosis induced by dexamethasone and talc |
| title_full | Pathogenetic features of experimental osteoarthrosis induced by dexamethasone and talc |
| title_fullStr | Pathogenetic features of experimental osteoarthrosis induced by dexamethasone and talc |
| title_full_unstemmed | Pathogenetic features of experimental osteoarthrosis induced by dexamethasone and talc |
| title_short | Pathogenetic features of experimental osteoarthrosis induced by dexamethasone and talc |
| title_sort | pathogenetic features of experimental osteoarthrosis induced by dexamethasone and talc |
| topic | остеоартроз крысы дексаметазон суставной хрящ хондроциты остеокальцин субхондральная кость гиалуроновая кислота коллаген i типа |
| url | https://bulletin.ssmu.ru/jour/article/view/4807 |
| work_keys_str_mv | AT evgladkova pathogeneticfeaturesofexperimentalosteoarthrosisinducedbydexamethasoneandtalc |