Altered microRNA profiles of testicular biopsies from patients with nonobstructive azoospermia
Many studies have shown that microRNAs (miRNAs) play vital roles during the spermatogenesis. However, little is known about the altered miRNA profiles of testicular tissues in nonobstructive azoospermia (NOA). Using microarray technology, the miRNA expression profiles of testicular biopsies from pat...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2020-01-01
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| Series: | Asian Journal of Andrology |
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| Online Access: | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2020;volume=22;issue=1;spage=100;epage=105;aulast=Zhang |
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| author | Hai-Tao Zhang Zhe Zhang Kai Hong Wen-Hao Tang De-Feng Liu Jia-Ming Mao Yu-Zhuo Yang Hao-Cheng Lin Hui Jiang |
| author_facet | Hai-Tao Zhang Zhe Zhang Kai Hong Wen-Hao Tang De-Feng Liu Jia-Ming Mao Yu-Zhuo Yang Hao-Cheng Lin Hui Jiang |
| author_sort | Hai-Tao Zhang |
| collection | DOAJ |
| description | Many studies have shown that microRNAs (miRNAs) play vital roles during the spermatogenesis. However, little is known about the altered miRNA profiles of testicular tissues in nonobstructive azoospermia (NOA). Using microarray technology, the miRNA expression profiles of testicular biopsies from patients with NOA and of normal testicular tissues were determined. Bioinformatics analyses were conducted to predict the enriched biological processes and functions of identified miRNAs. The microarray data were validated by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), the results of which were then validated with a larger sample size. Correlations between the miRNA expression levels and clinical characteristics were analyzed. Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic ability of miRNAs for azoospermia. Hierarchical clustering showed that 129 miRNAs were significantly differentially expressed between the NOA and control groups. Bioinformatics analysis indicated that the differentially expressed miRNAs were involved in spermatogenesis, cell cycle, and mitotic prometaphase. In the subsequent qRT-PCR assays, the selected miRNA expression levels were consistent with the microarray results, and similar validated results were obtained with a larger sample size. Some clinical characteristics were significantly associated with the expression of certain miRNAs. In particular, we identified a combination of two miRNAs (miR-10b-3p and miR-34b-5p) that could serve as a predictive biomarker of azoospermia. This study provides altered miRNA profiles of testicular biopsies from NOA patients and examines the roles of miRNAs in spermatogenesis. These profiles may be useful for predicting and diagnosing the presence of testicular sperm in individuals with azoospermia. |
| first_indexed | 2024-12-11T20:16:05Z |
| format | Article |
| id | doaj.art-6f760ac8687e43778199a9047ef6d3a3 |
| institution | Directory Open Access Journal |
| issn | 1008-682X 1745-7262 |
| language | English |
| last_indexed | 2024-12-11T20:16:05Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Andrology |
| spelling | doaj.art-6f760ac8687e43778199a9047ef6d3a32022-12-22T00:52:10ZengWolters Kluwer Medknow PublicationsAsian Journal of Andrology1008-682X1745-72622020-01-0122110010510.4103/aja.aja_35_19Altered microRNA profiles of testicular biopsies from patients with nonobstructive azoospermiaHai-Tao ZhangZhe ZhangKai HongWen-Hao TangDe-Feng LiuJia-Ming MaoYu-Zhuo YangHao-Cheng LinHui JiangMany studies have shown that microRNAs (miRNAs) play vital roles during the spermatogenesis. However, little is known about the altered miRNA profiles of testicular tissues in nonobstructive azoospermia (NOA). Using microarray technology, the miRNA expression profiles of testicular biopsies from patients with NOA and of normal testicular tissues were determined. Bioinformatics analyses were conducted to predict the enriched biological processes and functions of identified miRNAs. The microarray data were validated by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), the results of which were then validated with a larger sample size. Correlations between the miRNA expression levels and clinical characteristics were analyzed. Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic ability of miRNAs for azoospermia. Hierarchical clustering showed that 129 miRNAs were significantly differentially expressed between the NOA and control groups. Bioinformatics analysis indicated that the differentially expressed miRNAs were involved in spermatogenesis, cell cycle, and mitotic prometaphase. In the subsequent qRT-PCR assays, the selected miRNA expression levels were consistent with the microarray results, and similar validated results were obtained with a larger sample size. Some clinical characteristics were significantly associated with the expression of certain miRNAs. In particular, we identified a combination of two miRNAs (miR-10b-3p and miR-34b-5p) that could serve as a predictive biomarker of azoospermia. This study provides altered miRNA profiles of testicular biopsies from NOA patients and examines the roles of miRNAs in spermatogenesis. These profiles may be useful for predicting and diagnosing the presence of testicular sperm in individuals with azoospermia.http://www.ajandrology.com/article.asp?issn=1008-682X;year=2020;volume=22;issue=1;spage=100;epage=105;aulast=Zhangmale infertility; microrna; nonobstructive azoospermia; spermatogenesis |
| spellingShingle | Hai-Tao Zhang Zhe Zhang Kai Hong Wen-Hao Tang De-Feng Liu Jia-Ming Mao Yu-Zhuo Yang Hao-Cheng Lin Hui Jiang Altered microRNA profiles of testicular biopsies from patients with nonobstructive azoospermia Asian Journal of Andrology male infertility; microrna; nonobstructive azoospermia; spermatogenesis |
| title | Altered microRNA profiles of testicular biopsies from patients with nonobstructive azoospermia |
| title_full | Altered microRNA profiles of testicular biopsies from patients with nonobstructive azoospermia |
| title_fullStr | Altered microRNA profiles of testicular biopsies from patients with nonobstructive azoospermia |
| title_full_unstemmed | Altered microRNA profiles of testicular biopsies from patients with nonobstructive azoospermia |
| title_short | Altered microRNA profiles of testicular biopsies from patients with nonobstructive azoospermia |
| title_sort | altered microrna profiles of testicular biopsies from patients with nonobstructive azoospermia |
| topic | male infertility; microrna; nonobstructive azoospermia; spermatogenesis |
| url | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2020;volume=22;issue=1;spage=100;epage=105;aulast=Zhang |
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