Retinol and vitamin A metabolites accumulate through RBP4 and STRA6 changes in a psoriasis murine model
Abstract Background Psoriasis is a common chronic inflammatory skin disease that features the abnormal proliferation of keratinocytes. This proliferation could partly result from disturbances in vitamin A metabolism. Changes in psoriasis patients of the levels of retinol-binding protein 4 (RBP4), a...
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| Format: | Article |
| Language: | English |
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BMC
2020-01-01
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| Series: | Nutrition & Metabolism |
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| Online Access: | https://doi.org/10.1186/s12986-019-0423-y |
| _version_ | 1811270921405595648 |
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| author | Hai-meng Wang Chao Wu Yan-yun Jiang Wen-ming Wang Hong-zhong Jin |
| author_facet | Hai-meng Wang Chao Wu Yan-yun Jiang Wen-ming Wang Hong-zhong Jin |
| author_sort | Hai-meng Wang |
| collection | DOAJ |
| description | Abstract Background Psoriasis is a common chronic inflammatory skin disease that features the abnormal proliferation of keratinocytes. This proliferation could partly result from disturbances in vitamin A metabolism. Changes in psoriasis patients of the levels of retinol-binding protein 4 (RBP4), a carrier of retinol (vitamin A); transmembrane protein stimulated by retinoic acid 6 (STRA6); and other retinol metabolic molecules have not yet been fully established. Therefore, we investigated vitamin A-related proteins in mice with imiquimod (IMQ)-induced psoriasis. Methods Thirty mice were divided into four study groups: two groups underwent IMQ application for 3 or 6 days (groups A and B, respectively), and two groups underwent Vaseline application for 3 or 6 days (groups C and D, respectively). Blood and skin samples from both lesional and non-lesional areas of the mice were analyzed using enzyme-linked immunosorbent assays, hematoxylin and eosin staining, immunochemistry, real-time reverse transcription polymerase chain reaction, and RNA sequencing. Results IMQ-treated mice developed erythema, scales, and skin thickening. Compared with the control groups, IMQ-treated groups had the following changes: 1) interleukin (IL)-17A, IL-23, and tumor necrosis factor (TNF)-α levels were raised significantly in both serum and lesional skin (all p < 0.001); 2) retinol levels in lesional skin increased slightly (p = 0.364), but no change was evident in serum retinol levels; 3) STRA6 was upregulated in both lesional skin (p = 0.021) and serum (p = 0.034); 4) RBP4 levels were elevated in serum (p = 0.042), but exhibited only an increasing trend (p = 0.273) in lesional skin; and 5) proteins and enzymes that mediate retinoic acid formation and transformation were upregulated in lesional skin. Conclusions As the demand for vitamin A in psoriatic mice increased, retinol underwent relocation from the circulation to target tissues. RBP4, STRA6, and the transformation from retinol to retinoic acid were upregulated, which may be part of the mechanism of psoriasis skin lesion formation. We propose that a positive feedback mechanism was formed that maintained the severity of psoriasis. |
| first_indexed | 2024-04-12T22:11:56Z |
| format | Article |
| id | doaj.art-6f764000343648ddb20177c7f4a72b4a |
| institution | Directory Open Access Journal |
| issn | 1743-7075 |
| language | English |
| last_indexed | 2024-04-12T22:11:56Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Nutrition & Metabolism |
| spelling | doaj.art-6f764000343648ddb20177c7f4a72b4a2022-12-22T03:14:43ZengBMCNutrition & Metabolism1743-70752020-01-0117111510.1186/s12986-019-0423-yRetinol and vitamin A metabolites accumulate through RBP4 and STRA6 changes in a psoriasis murine modelHai-meng Wang0Chao Wu1Yan-yun Jiang2Wen-ming Wang3Hong-zhong Jin4Department of Dermatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, National Clinical Research Center for Skin and Immune DiseasesDepartment of Dermatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, National Clinical Research Center for Skin and Immune DiseasesDepartment of Dermatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, National Clinical Research Center for Skin and Immune DiseasesDepartment of Dermatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, National Clinical Research Center for Skin and Immune DiseasesDepartment of Dermatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, National Clinical Research Center for Skin and Immune DiseasesAbstract Background Psoriasis is a common chronic inflammatory skin disease that features the abnormal proliferation of keratinocytes. This proliferation could partly result from disturbances in vitamin A metabolism. Changes in psoriasis patients of the levels of retinol-binding protein 4 (RBP4), a carrier of retinol (vitamin A); transmembrane protein stimulated by retinoic acid 6 (STRA6); and other retinol metabolic molecules have not yet been fully established. Therefore, we investigated vitamin A-related proteins in mice with imiquimod (IMQ)-induced psoriasis. Methods Thirty mice were divided into four study groups: two groups underwent IMQ application for 3 or 6 days (groups A and B, respectively), and two groups underwent Vaseline application for 3 or 6 days (groups C and D, respectively). Blood and skin samples from both lesional and non-lesional areas of the mice were analyzed using enzyme-linked immunosorbent assays, hematoxylin and eosin staining, immunochemistry, real-time reverse transcription polymerase chain reaction, and RNA sequencing. Results IMQ-treated mice developed erythema, scales, and skin thickening. Compared with the control groups, IMQ-treated groups had the following changes: 1) interleukin (IL)-17A, IL-23, and tumor necrosis factor (TNF)-α levels were raised significantly in both serum and lesional skin (all p < 0.001); 2) retinol levels in lesional skin increased slightly (p = 0.364), but no change was evident in serum retinol levels; 3) STRA6 was upregulated in both lesional skin (p = 0.021) and serum (p = 0.034); 4) RBP4 levels were elevated in serum (p = 0.042), but exhibited only an increasing trend (p = 0.273) in lesional skin; and 5) proteins and enzymes that mediate retinoic acid formation and transformation were upregulated in lesional skin. Conclusions As the demand for vitamin A in psoriatic mice increased, retinol underwent relocation from the circulation to target tissues. RBP4, STRA6, and the transformation from retinol to retinoic acid were upregulated, which may be part of the mechanism of psoriasis skin lesion formation. We propose that a positive feedback mechanism was formed that maintained the severity of psoriasis.https://doi.org/10.1186/s12986-019-0423-yPsoriasisMouseRetinolRBP4STRA6 |
| spellingShingle | Hai-meng Wang Chao Wu Yan-yun Jiang Wen-ming Wang Hong-zhong Jin Retinol and vitamin A metabolites accumulate through RBP4 and STRA6 changes in a psoriasis murine model Nutrition & Metabolism Psoriasis Mouse Retinol RBP4 STRA6 |
| title | Retinol and vitamin A metabolites accumulate through RBP4 and STRA6 changes in a psoriasis murine model |
| title_full | Retinol and vitamin A metabolites accumulate through RBP4 and STRA6 changes in a psoriasis murine model |
| title_fullStr | Retinol and vitamin A metabolites accumulate through RBP4 and STRA6 changes in a psoriasis murine model |
| title_full_unstemmed | Retinol and vitamin A metabolites accumulate through RBP4 and STRA6 changes in a psoriasis murine model |
| title_short | Retinol and vitamin A metabolites accumulate through RBP4 and STRA6 changes in a psoriasis murine model |
| title_sort | retinol and vitamin a metabolites accumulate through rbp4 and stra6 changes in a psoriasis murine model |
| topic | Psoriasis Mouse Retinol RBP4 STRA6 |
| url | https://doi.org/10.1186/s12986-019-0423-y |
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