| Summary: | The objectives of this research were to (1) determine the feed intake, digestibility, and energy utilization and (2) estimate the net energy value of cassava chips consumed by lactating dairy cows. Four multiparous Holstein–Friesian crossbred cows at 139 ± 33 (mean ± SD) day in milk were assigned according to a 4 × 4 Latin square design with four periods. The four treatments included a diet substituted with cassava chips on a 0%, 12%, 24%, and 36% dry matter (DM) basis in the basal diet. Indirect calorimetry with a head cage respiration system was used to determine nutrient and energy utilization. Increasing the number of cassava chips in the diet resulted in a linear increase (<i>p</i> < 0.05) in nutrient intake and digestibility but a linear decrease (<i>p</i> < 0.01) in crude protein (CP) and fiber. The enteric methane yield and intensity were not affected (<i>p</i> > 0.05), while energy was lost as feces and urine reduced linearly (<i>p</i> < 0.05). Milk yield and milk composition (protein, fat, lactose) also increased linearly (<i>p</i> < 0.05). The net energy requirement for the maintenance of the lactating cows was estimated as 327 kJ/kg of metabolic body weight, and the efficiency of metabolizable energy used for lactation was 0.66. The estimated net energy value of cassava chips for lactation was 8.03 MJ/kg DM.
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