Protective Effect of Cannabinoid Type 2 Receptor Agonist Against Ovarian Ischemia/reperfusion Injury in Rats
Objective: The aim of this study is to prove whether or not the cannabinoid (CB)2 receptor agonist, which is known to be effective in anti-inflammatory effects and tried in various organ and/or tissue ischemia/reperfusion (I/R) injuries, works in the treatment of I/R injury, which is a replication o...
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| Format: | Article |
| Language: | English |
| Published: |
Sakarya University
2019-12-01
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| Series: | Sakarya Tıp Dergisi |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/download/article-file/881658 |
| _version_ | 1797350340078600192 |
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| author | Ali Parlar Buket Bilgin Şahin |
| author_facet | Ali Parlar Buket Bilgin Şahin |
| author_sort | Ali Parlar |
| collection | DOAJ |
| description | Objective: The aim of this study is to prove whether or not the cannabinoid
(CB)2 receptor agonist, which is known to be effective in
anti-inflammatory effects and tried in various organ and/or tissue ischemia/reperfusion
(I/R) injuries, works in the treatment of I/R injury, which is a replication of
the damage caused by clinical ovarian torsion.Materials
and Methods: Rats were divided
into five groups (n=6). After anesthesia, an approximately 2-cm incision was
made in the lower abdominal region of the rats and both ovaries, uterine horns,
and adnexa were occluded with a clamp for 30 min, except for the sham control group.
After 3 h, both ovaries were surgically removed. Blood was collected for the
measurement of cytokine levels in the serum.Results:
The induction of ovaries by
I/R injury resulted in an increase in interleukin 1 beta, tumor necrosis factor
alpha, and malondialdehyde levels and decrease in glutathione (GSH) levels in
ovarian tissues and serum. The pretreatment of the CB2
receptor agonist decreased these cytokines levels in both ovarian tissues and
serum. The GSH level in both was reduced by I/R injury, while the agonist
increased the GSH level reduced by I/R injury. The administration of the
antagonist reversed the curative effect of the agonist.
Conclusion: The results of this study support that the CB2
receptor agonist can be used to treat I/R injury, which is an imitation of
ovarian torsion. However, no the clinical trial is a limitation of this study. |
| first_indexed | 2024-03-08T12:43:22Z |
| format | Article |
| id | doaj.art-6f807f7a5898452a8583882766314204 |
| institution | Directory Open Access Journal |
| issn | 2146-409X |
| language | English |
| last_indexed | 2024-03-08T12:43:22Z |
| publishDate | 2019-12-01 |
| publisher | Sakarya University |
| record_format | Article |
| series | Sakarya Tıp Dergisi |
| spelling | doaj.art-6f807f7a5898452a85838827663142042024-01-21T07:40:12ZengSakarya UniversitySakarya Tıp Dergisi2146-409X2019-12-019467968610.31832/smj.62620428Protective Effect of Cannabinoid Type 2 Receptor Agonist Against Ovarian Ischemia/reperfusion Injury in RatsAli Parlar0Buket Bilgin Şahin1ADIYAMAN UNIVERSITYADIYAMAN GÖLBAŞI DEVLET HASTANESİObjective: The aim of this study is to prove whether or not the cannabinoid (CB)2 receptor agonist, which is known to be effective in anti-inflammatory effects and tried in various organ and/or tissue ischemia/reperfusion (I/R) injuries, works in the treatment of I/R injury, which is a replication of the damage caused by clinical ovarian torsion.Materials and Methods: Rats were divided into five groups (n=6). After anesthesia, an approximately 2-cm incision was made in the lower abdominal region of the rats and both ovaries, uterine horns, and adnexa were occluded with a clamp for 30 min, except for the sham control group. After 3 h, both ovaries were surgically removed. Blood was collected for the measurement of cytokine levels in the serum.Results: The induction of ovaries by I/R injury resulted in an increase in interleukin 1 beta, tumor necrosis factor alpha, and malondialdehyde levels and decrease in glutathione (GSH) levels in ovarian tissues and serum. The pretreatment of the CB2 receptor agonist decreased these cytokines levels in both ovarian tissues and serum. The GSH level in both was reduced by I/R injury, while the agonist increased the GSH level reduced by I/R injury. The administration of the antagonist reversed the curative effect of the agonist. Conclusion: The results of this study support that the CB2 receptor agonist can be used to treat I/R injury, which is an imitation of ovarian torsion. However, no the clinical trial is a limitation of this study.https://dergipark.org.tr/tr/download/article-file/881658i/ram1241ovarian torsioncytokinesam630i/ram1241ovaryum torsiyonusitokinleram630 |
| spellingShingle | Ali Parlar Buket Bilgin Şahin Protective Effect of Cannabinoid Type 2 Receptor Agonist Against Ovarian Ischemia/reperfusion Injury in Rats Sakarya Tıp Dergisi i/r am1241 ovarian torsion cytokines am630 i/r am1241 ovaryum torsiyonu sitokinler am630 |
| title | Protective Effect of Cannabinoid Type 2 Receptor Agonist Against Ovarian Ischemia/reperfusion Injury in Rats |
| title_full | Protective Effect of Cannabinoid Type 2 Receptor Agonist Against Ovarian Ischemia/reperfusion Injury in Rats |
| title_fullStr | Protective Effect of Cannabinoid Type 2 Receptor Agonist Against Ovarian Ischemia/reperfusion Injury in Rats |
| title_full_unstemmed | Protective Effect of Cannabinoid Type 2 Receptor Agonist Against Ovarian Ischemia/reperfusion Injury in Rats |
| title_short | Protective Effect of Cannabinoid Type 2 Receptor Agonist Against Ovarian Ischemia/reperfusion Injury in Rats |
| title_sort | protective effect of cannabinoid type 2 receptor agonist against ovarian ischemia reperfusion injury in rats |
| topic | i/r am1241 ovarian torsion cytokines am630 i/r am1241 ovaryum torsiyonu sitokinler am630 |
| url | https://dergipark.org.tr/tr/download/article-file/881658 |
| work_keys_str_mv | AT aliparlar protectiveeffectofcannabinoidtype2receptoragonistagainstovarianischemiareperfusioninjuryinrats AT buketbilginsahin protectiveeffectofcannabinoidtype2receptoragonistagainstovarianischemiareperfusioninjuryinrats |