Uji Aktivitas Antibakteri Fraksi Etil Asetat Daun Tanaman Bandotan (Ageratum conyzoides L)
Utilization of Ageratum conyzoides L. leaf for wound healing and digestion disorders are often associated with its antibacterial activities. However, informastion of this plant as antibacterial agent still limited on the polar and non polar fraction. Therefore, the objective of this study was to ide...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin
2016-03-01
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| Series: | JIIS: Jurnal Ilmiah Ibnu Sina |
| Subjects: | |
| Online Access: | http://jiis.akfar-isfibjm.ac.id/index.php/JIIS/article/view/34/29 |
| _version_ | 1811193770315612160 |
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| author | Taufan H. Sugara Tun Tedja Irawadi Irma Herawati Suprapto Muhammad Hanafi |
| author_facet | Taufan H. Sugara Tun Tedja Irawadi Irma Herawati Suprapto Muhammad Hanafi |
| author_sort | Taufan H. Sugara |
| collection | DOAJ |
| description | Utilization of Ageratum conyzoides L. leaf for wound healing and digestion disorders are often associated with its antibacterial activities. However, informastion of this plant as antibacterial agent still limited on the polar and non polar fraction. Therefore, the objective of this study was to identify the antibacterial activity of semipolar fraktion extract of Ageratum conyzoides L. leaves to Staphylococcus aureus and Eschirichia coli. Phytochemical test results showed that the ethyl acetate extract of bandotan leaves contains flavonoids, steroids, p-hydroquinone and terpenoids. Fractionation of ethyl acetate extract with eluent chloroform: methanol (9:1) produced 8 fractions by the number of consecutive yield as much 15,14g; 22,21g; 21,19g; 20,44g; 5,79g; 4,50g; 1,40g; 1,43g; and 0,93g. Overal antibakterial activity result of ethyl acetat extract and fraction 1-8 showed broad-spectrum antibacterial activity with inhibition of belonging to the strong category. The average inhibition of ethyl acetate extracts and fractions 1-8 against S. aureus in a row amounted to 14mm; 12mm; 12,5mm; 15,5mm; 16mm; 12mm; 11,5mm; 10mm; 9,5mm. While, the average inhibition of ethyl acetate extracts and fractions 1-8 against E. coli in a row amounted to 11mm; 11,5mm; 11mm; 11,5mm; 13mm; 14mm; 10.5 mm; 10mm; 9,5mm. |
| first_indexed | 2024-04-12T00:14:56Z |
| format | Article |
| id | doaj.art-6f80a536ba674faf9689533b741fa19c |
| institution | Directory Open Access Journal |
| issn | 2502-647X 2503-1902 |
| language | English |
| last_indexed | 2024-04-12T00:14:56Z |
| publishDate | 2016-03-01 |
| publisher | Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin |
| record_format | Article |
| series | JIIS: Jurnal Ilmiah Ibnu Sina |
| spelling | doaj.art-6f80a536ba674faf9689533b741fa19c2022-12-22T03:55:52ZengSekolah Tinggi Ilmu Kesehatan ISFI BanjarmasinJIIS: Jurnal Ilmiah Ibnu Sina2502-647X2503-19022016-03-01118896Uji Aktivitas Antibakteri Fraksi Etil Asetat Daun Tanaman Bandotan (Ageratum conyzoides L)Taufan H. Sugara0Tun Tedja Irawadi1Irma Herawati Suprapto2Muhammad Hanafi3Fakultas Ilmu Kesehatan Universitas Muhammadiyah MataramDepartemen Kimia IPBDepartemen Kimia IPBLIPIUtilization of Ageratum conyzoides L. leaf for wound healing and digestion disorders are often associated with its antibacterial activities. However, informastion of this plant as antibacterial agent still limited on the polar and non polar fraction. Therefore, the objective of this study was to identify the antibacterial activity of semipolar fraktion extract of Ageratum conyzoides L. leaves to Staphylococcus aureus and Eschirichia coli. Phytochemical test results showed that the ethyl acetate extract of bandotan leaves contains flavonoids, steroids, p-hydroquinone and terpenoids. Fractionation of ethyl acetate extract with eluent chloroform: methanol (9:1) produced 8 fractions by the number of consecutive yield as much 15,14g; 22,21g; 21,19g; 20,44g; 5,79g; 4,50g; 1,40g; 1,43g; and 0,93g. Overal antibakterial activity result of ethyl acetat extract and fraction 1-8 showed broad-spectrum antibacterial activity with inhibition of belonging to the strong category. The average inhibition of ethyl acetate extracts and fractions 1-8 against S. aureus in a row amounted to 14mm; 12mm; 12,5mm; 15,5mm; 16mm; 12mm; 11,5mm; 10mm; 9,5mm. While, the average inhibition of ethyl acetate extracts and fractions 1-8 against E. coli in a row amounted to 11mm; 11,5mm; 11mm; 11,5mm; 13mm; 14mm; 10.5 mm; 10mm; 9,5mm.http://jiis.akfar-isfibjm.ac.id/index.php/JIIS/article/view/34/29Ageratum conyzoides leafethyl acetat fractionantibacterial |
| spellingShingle | Taufan H. Sugara Tun Tedja Irawadi Irma Herawati Suprapto Muhammad Hanafi Uji Aktivitas Antibakteri Fraksi Etil Asetat Daun Tanaman Bandotan (Ageratum conyzoides L) JIIS: Jurnal Ilmiah Ibnu Sina Ageratum conyzoides leaf ethyl acetat fraction antibacterial |
| title | Uji Aktivitas Antibakteri Fraksi Etil Asetat Daun Tanaman Bandotan (Ageratum conyzoides L) |
| title_full | Uji Aktivitas Antibakteri Fraksi Etil Asetat Daun Tanaman Bandotan (Ageratum conyzoides L) |
| title_fullStr | Uji Aktivitas Antibakteri Fraksi Etil Asetat Daun Tanaman Bandotan (Ageratum conyzoides L) |
| title_full_unstemmed | Uji Aktivitas Antibakteri Fraksi Etil Asetat Daun Tanaman Bandotan (Ageratum conyzoides L) |
| title_short | Uji Aktivitas Antibakteri Fraksi Etil Asetat Daun Tanaman Bandotan (Ageratum conyzoides L) |
| title_sort | uji aktivitas antibakteri fraksi etil asetat daun tanaman bandotan ageratum conyzoides l |
| topic | Ageratum conyzoides leaf ethyl acetat fraction antibacterial |
| url | http://jiis.akfar-isfibjm.ac.id/index.php/JIIS/article/view/34/29 |
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