A rapid and reliable detection procedure of Atlantic trout introgression at the diagnostic lactate dehydrogenase chain‐1 gene
Abstract The Italian‐native Mediterranean brown trout (Salmo ghigii) is a seriously threatened freshwater fish, especially by anthropogenic hybridisation with the domestic strains of Atlantic origin that have been repeatedly released into the wild for angling. A PCR‐restriction fragment length polym...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-08-01
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| Series: | Aquaculture, Fish and Fisheries |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/aff2.124 |
| _version_ | 1797768068933353472 |
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| author | Anna Padula Claudia Greco Lorenzo Talarico Romolo Caniglia Caterina Maria Antognazza Susanna D'Antoni Massimo Lorenzoni Isabella Vanetti Serena Zaccara Nadia Mucci |
| author_facet | Anna Padula Claudia Greco Lorenzo Talarico Romolo Caniglia Caterina Maria Antognazza Susanna D'Antoni Massimo Lorenzoni Isabella Vanetti Serena Zaccara Nadia Mucci |
| author_sort | Anna Padula |
| collection | DOAJ |
| description | Abstract The Italian‐native Mediterranean brown trout (Salmo ghigii) is a seriously threatened freshwater fish, especially by anthropogenic hybridisation with the domestic strains of Atlantic origin that have been repeatedly released into the wild for angling. A PCR‐restriction fragment length polymorphism (RFLP) assay of the diagnostic lactate dehydrogenase chain‐1 (LDH‐C1) gene sequences has been routinely applied to distinguish exotic from native brown trout lineages and detect Atlantic introgression signals in the Mediterranean wild populations. Here, we used dermal swab DNA obtained from 28 wild trout to improve laboratory procedures to genetically characterise trout samples at the LDH‐C1gene through (1) a capillary electrophoresis analysis of the RFLP fragments and (2) the optimisation of a diagnostic single nucleotide polymorphism analysable through mini‐sequencing approaches. The developed methods were fully consistent with those obtained through the traditional approach, but their analytical process is almost entirely automated and digitalised, thus improving result readability and accuracy in the detection of alien introgressed traces in wild Mediterranean brown trout populations. |
| first_indexed | 2024-03-12T20:49:13Z |
| format | Article |
| id | doaj.art-6f80cbc9a0414ba193f6a123df75f81f |
| institution | Directory Open Access Journal |
| issn | 2693-8847 |
| language | English |
| last_indexed | 2024-03-12T20:49:13Z |
| publishDate | 2023-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aquaculture, Fish and Fisheries |
| spelling | doaj.art-6f80cbc9a0414ba193f6a123df75f81f2023-08-01T06:34:40ZengWileyAquaculture, Fish and Fisheries2693-88472023-08-013438839210.1002/aff2.124A rapid and reliable detection procedure of Atlantic trout introgression at the diagnostic lactate dehydrogenase chain‐1 geneAnna Padula0Claudia Greco1Lorenzo Talarico2Romolo Caniglia3Caterina Maria Antognazza4Susanna D'Antoni5Massimo Lorenzoni6Isabella Vanetti7Serena Zaccara8Nadia Mucci9Unit for Conservation Genetics (BIO‐CGE), Department for the Monitoring and Protection of the Environment and for Biodiversity Conservation Italian Institute for Environmental Protection and Research (ISPRA) Bologna ItalyUnit for Conservation Genetics (BIO‐CGE), Department for the Monitoring and Protection of the Environment and for Biodiversity Conservation Italian Institute for Environmental Protection and Research (ISPRA) Bologna ItalyUnit for Conservation Genetics (BIO‐CGE), Department for the Monitoring and Protection of the Environment and for Biodiversity Conservation Italian Institute for Environmental Protection and Research (ISPRA) Bologna ItalyUnit for Conservation Genetics (BIO‐CGE), Department for the Monitoring and Protection of the Environment and for Biodiversity Conservation Italian Institute for Environmental Protection and Research (ISPRA) Bologna ItalyDepartment of Theoretical and Applied Science University of Insubria Varese ItalyDivision for Sustainable Land‐Use Planning, Protected Areas, and Landscape Conservation, Nature and Terrestrial Ecosystem Services (BIO‐SOST), Department for the Monitoring and Protection of the Environment and for Biodiversity Conservation Italian Institute for Environmental Protection and Research (ISPRA) Rome ItalyDepartment of Chemistry, Biology and Biotechnologies University of Perugia Perugia ItalyDepartment of Theoretical and Applied Science University of Insubria Varese ItalyDepartment of Theoretical and Applied Science University of Insubria Varese ItalyUnit for Conservation Genetics (BIO‐CGE), Department for the Monitoring and Protection of the Environment and for Biodiversity Conservation Italian Institute for Environmental Protection and Research (ISPRA) Bologna ItalyAbstract The Italian‐native Mediterranean brown trout (Salmo ghigii) is a seriously threatened freshwater fish, especially by anthropogenic hybridisation with the domestic strains of Atlantic origin that have been repeatedly released into the wild for angling. A PCR‐restriction fragment length polymorphism (RFLP) assay of the diagnostic lactate dehydrogenase chain‐1 (LDH‐C1) gene sequences has been routinely applied to distinguish exotic from native brown trout lineages and detect Atlantic introgression signals in the Mediterranean wild populations. Here, we used dermal swab DNA obtained from 28 wild trout to improve laboratory procedures to genetically characterise trout samples at the LDH‐C1gene through (1) a capillary electrophoresis analysis of the RFLP fragments and (2) the optimisation of a diagnostic single nucleotide polymorphism analysable through mini‐sequencing approaches. The developed methods were fully consistent with those obtained through the traditional approach, but their analytical process is almost entirely automated and digitalised, thus improving result readability and accuracy in the detection of alien introgressed traces in wild Mediterranean brown trout populations.https://doi.org/10.1002/aff2.124dermal swabfishLDH‐C1 genotypingnon‐invasive genetic samplingSalmo trutta complexSNP |
| spellingShingle | Anna Padula Claudia Greco Lorenzo Talarico Romolo Caniglia Caterina Maria Antognazza Susanna D'Antoni Massimo Lorenzoni Isabella Vanetti Serena Zaccara Nadia Mucci A rapid and reliable detection procedure of Atlantic trout introgression at the diagnostic lactate dehydrogenase chain‐1 gene Aquaculture, Fish and Fisheries dermal swab fish LDH‐C1 genotyping non‐invasive genetic sampling Salmo trutta complex SNP |
| title | A rapid and reliable detection procedure of Atlantic trout introgression at the diagnostic lactate dehydrogenase chain‐1 gene |
| title_full | A rapid and reliable detection procedure of Atlantic trout introgression at the diagnostic lactate dehydrogenase chain‐1 gene |
| title_fullStr | A rapid and reliable detection procedure of Atlantic trout introgression at the diagnostic lactate dehydrogenase chain‐1 gene |
| title_full_unstemmed | A rapid and reliable detection procedure of Atlantic trout introgression at the diagnostic lactate dehydrogenase chain‐1 gene |
| title_short | A rapid and reliable detection procedure of Atlantic trout introgression at the diagnostic lactate dehydrogenase chain‐1 gene |
| title_sort | rapid and reliable detection procedure of atlantic trout introgression at the diagnostic lactate dehydrogenase chain 1 gene |
| topic | dermal swab fish LDH‐C1 genotyping non‐invasive genetic sampling Salmo trutta complex SNP |
| url | https://doi.org/10.1002/aff2.124 |
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