Viral labeling of neurons synaptically connected to nucleus accumbens somatostatin interneurons.
The nucleus accumbens, a key brain reward region, receives synaptic inputs from a range of forebrain and brainstem regions. Many of these projections have been established using electrophysiology or fluorescent tract tracing. However, more recently developed viral tracing techniques have allowed for...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0213476 |
| _version_ | 1818989621779890176 |
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| author | Efrain A Ribeiro Alexander R Nectow Lisa E Pomeranz Mats I Ekstrand Ja Wook Koo Eric J Nestler |
| author_facet | Efrain A Ribeiro Alexander R Nectow Lisa E Pomeranz Mats I Ekstrand Ja Wook Koo Eric J Nestler |
| author_sort | Efrain A Ribeiro |
| collection | DOAJ |
| description | The nucleus accumbens, a key brain reward region, receives synaptic inputs from a range of forebrain and brainstem regions. Many of these projections have been established using electrophysiology or fluorescent tract tracing. However, more recently developed viral tracing techniques have allowed for fluorescent labeling of synaptic afferents in a cell type-specific manner. Since the NAc is comprised of multiple cell types, these methods have enabled the delineation of the cell type-specific connectivity of principal medium spiny neurons in the region. The synaptic connectivity of somatostatin interneurons, which account for <5% of the neurons in the region, has been inferred from electrophysiological and immunohistochemical data, but has not yet been visualized using modern viral tracing techniques. Here, we use the pseudorabies virus (PRV)-Introvert-GFP virus, an alphaherpes virus previously shown to label synaptic afferents in a cell type-specific manner, to label first order afferents to NAc somatostatin interneurons. While we find GFP(+) labeling in several well established projections to the NAc, we also observe that several known projections to NAc did not contain GFP(+) cells, suggesting they do not innervate somatostatin interneurons in the region. A subset of the GFP(+) afferents are c-FOS(+) following acute administration of cocaine, showing that NAc somatostatin interneurons are innervated by some cells that respond to rewarding stimuli. These results provide a foundation for future studies aimed toward elucidating the cell type-specific connectivity of the NAc, and identify specific circuits that warrant future functional characterization. |
| first_indexed | 2024-12-20T19:41:24Z |
| format | Article |
| id | doaj.art-6f83eb701bf1495286bf6aaa35c8984a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T19:41:24Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6f83eb701bf1495286bf6aaa35c8984a2022-12-21T19:28:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01143e021347610.1371/journal.pone.0213476Viral labeling of neurons synaptically connected to nucleus accumbens somatostatin interneurons.Efrain A RibeiroAlexander R NectowLisa E PomeranzMats I EkstrandJa Wook KooEric J NestlerThe nucleus accumbens, a key brain reward region, receives synaptic inputs from a range of forebrain and brainstem regions. Many of these projections have been established using electrophysiology or fluorescent tract tracing. However, more recently developed viral tracing techniques have allowed for fluorescent labeling of synaptic afferents in a cell type-specific manner. Since the NAc is comprised of multiple cell types, these methods have enabled the delineation of the cell type-specific connectivity of principal medium spiny neurons in the region. The synaptic connectivity of somatostatin interneurons, which account for <5% of the neurons in the region, has been inferred from electrophysiological and immunohistochemical data, but has not yet been visualized using modern viral tracing techniques. Here, we use the pseudorabies virus (PRV)-Introvert-GFP virus, an alphaherpes virus previously shown to label synaptic afferents in a cell type-specific manner, to label first order afferents to NAc somatostatin interneurons. While we find GFP(+) labeling in several well established projections to the NAc, we also observe that several known projections to NAc did not contain GFP(+) cells, suggesting they do not innervate somatostatin interneurons in the region. A subset of the GFP(+) afferents are c-FOS(+) following acute administration of cocaine, showing that NAc somatostatin interneurons are innervated by some cells that respond to rewarding stimuli. These results provide a foundation for future studies aimed toward elucidating the cell type-specific connectivity of the NAc, and identify specific circuits that warrant future functional characterization.https://doi.org/10.1371/journal.pone.0213476 |
| spellingShingle | Efrain A Ribeiro Alexander R Nectow Lisa E Pomeranz Mats I Ekstrand Ja Wook Koo Eric J Nestler Viral labeling of neurons synaptically connected to nucleus accumbens somatostatin interneurons. PLoS ONE |
| title | Viral labeling of neurons synaptically connected to nucleus accumbens somatostatin interneurons. |
| title_full | Viral labeling of neurons synaptically connected to nucleus accumbens somatostatin interneurons. |
| title_fullStr | Viral labeling of neurons synaptically connected to nucleus accumbens somatostatin interneurons. |
| title_full_unstemmed | Viral labeling of neurons synaptically connected to nucleus accumbens somatostatin interneurons. |
| title_short | Viral labeling of neurons synaptically connected to nucleus accumbens somatostatin interneurons. |
| title_sort | viral labeling of neurons synaptically connected to nucleus accumbens somatostatin interneurons |
| url | https://doi.org/10.1371/journal.pone.0213476 |
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