Untargeted Metabolomic Approach to Study the Impact of Aging on Salivary Metabolome in Women
Despite the growing interest in salivary metabolomics, few studies have investigated the impact of aging on the salivary metabolome. The alterations in metabolic pathways that occur with aging are likely to be observed in pathologies affecting older people and may interfere with the search for saliv...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/12/10/986 |
| _version_ | 1827648891083816960 |
|---|---|
| author | Pauline Bosman Valérie Pichon Ana Carolina Acevedo Laëtitia Le Pottier Jacques Olivier Pers Hélène Chardin Audrey Combès |
| author_facet | Pauline Bosman Valérie Pichon Ana Carolina Acevedo Laëtitia Le Pottier Jacques Olivier Pers Hélène Chardin Audrey Combès |
| author_sort | Pauline Bosman |
| collection | DOAJ |
| description | Despite the growing interest in salivary metabolomics, few studies have investigated the impact of aging on the salivary metabolome. The alterations in metabolic pathways that occur with aging are likely to be observed in pathologies affecting older people and may interfere with the search for salivary biomarkers. It is therefore important to investigate the age-related changes occurring in the salivary metabolome. Using reversed phase liquid chromatography and hydrophilic interaction chromatography coupled to mass spectrometry used in positive and negative ionization modes, the salivary metabolic profiles of young (22 to 45 years old) and older people (55 to 92 years old) were obtained. Those profiles were compared with the use of XCMS online to highlight the under or overexpression of some metabolites with aging. A total of 60 metabolites showed differential expression with age. The identification of 26 of them was proposed by the METLIN database and, among them, 17 were validated by standard injections. Aging seemed to affect most of the main metabolic pathways (amino acid metabolism, Krebs cycle, fatty acid synthesis, and nucleic acid synthesis). Moreover, most of the metabolites that were over- or under-expressed with age in this study have already been identified as being potential biomarkers of diseases affecting older people, such as in Alzheimer’s disease. Special attention should be paid in the search for biomarkers of pathologies affecting the elderly to differentiate age-related changes from disease-related changes. |
| first_indexed | 2024-03-09T19:49:14Z |
| format | Article |
| id | doaj.art-6f85710fae6c46bfa2bf6966389cbe68 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T19:49:14Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-6f85710fae6c46bfa2bf6966389cbe682023-11-24T01:16:24ZengMDPI AGMetabolites2218-19892022-10-01121098610.3390/metabo12100986Untargeted Metabolomic Approach to Study the Impact of Aging on Salivary Metabolome in WomenPauline Bosman0Valérie Pichon1Ana Carolina Acevedo2Laëtitia Le Pottier3Jacques Olivier Pers4Hélène Chardin5Audrey Combès6Laboratoire des Sciences Analytiques, Bioanalytiques et Miniaturisation, UMR 8231 CBI CNRS, ESPCI Paris, PSL Université, 75005 Paris, FranceLaboratoire des Sciences Analytiques, Bioanalytiques et Miniaturisation, UMR 8231 CBI CNRS, ESPCI Paris, PSL Université, 75005 Paris, FranceLaboratory of Oral Histopathology, Health Sciences Faculty, University of Brasilia, Brasília DF CEP 70910-900, BrazilLBAI, UMR 1227, Université de Brest, Inserm, 29200 Brest, FranceLBAI, UMR 1227, Université de Brest, Inserm, 29200 Brest, FranceLaboratoire des Sciences Analytiques, Bioanalytiques et Miniaturisation, UMR 8231 CBI CNRS, ESPCI Paris, PSL Université, 75005 Paris, FranceLaboratoire des Sciences Analytiques, Bioanalytiques et Miniaturisation, UMR 8231 CBI CNRS, ESPCI Paris, PSL Université, 75005 Paris, FranceDespite the growing interest in salivary metabolomics, few studies have investigated the impact of aging on the salivary metabolome. The alterations in metabolic pathways that occur with aging are likely to be observed in pathologies affecting older people and may interfere with the search for salivary biomarkers. It is therefore important to investigate the age-related changes occurring in the salivary metabolome. Using reversed phase liquid chromatography and hydrophilic interaction chromatography coupled to mass spectrometry used in positive and negative ionization modes, the salivary metabolic profiles of young (22 to 45 years old) and older people (55 to 92 years old) were obtained. Those profiles were compared with the use of XCMS online to highlight the under or overexpression of some metabolites with aging. A total of 60 metabolites showed differential expression with age. The identification of 26 of them was proposed by the METLIN database and, among them, 17 were validated by standard injections. Aging seemed to affect most of the main metabolic pathways (amino acid metabolism, Krebs cycle, fatty acid synthesis, and nucleic acid synthesis). Moreover, most of the metabolites that were over- or under-expressed with age in this study have already been identified as being potential biomarkers of diseases affecting older people, such as in Alzheimer’s disease. Special attention should be paid in the search for biomarkers of pathologies affecting the elderly to differentiate age-related changes from disease-related changes.https://www.mdpi.com/2218-1989/12/10/986LC-HRMSsalivary metabolomeaging |
| spellingShingle | Pauline Bosman Valérie Pichon Ana Carolina Acevedo Laëtitia Le Pottier Jacques Olivier Pers Hélène Chardin Audrey Combès Untargeted Metabolomic Approach to Study the Impact of Aging on Salivary Metabolome in Women Metabolites LC-HRMS salivary metabolome aging |
| title | Untargeted Metabolomic Approach to Study the Impact of Aging on Salivary Metabolome in Women |
| title_full | Untargeted Metabolomic Approach to Study the Impact of Aging on Salivary Metabolome in Women |
| title_fullStr | Untargeted Metabolomic Approach to Study the Impact of Aging on Salivary Metabolome in Women |
| title_full_unstemmed | Untargeted Metabolomic Approach to Study the Impact of Aging on Salivary Metabolome in Women |
| title_short | Untargeted Metabolomic Approach to Study the Impact of Aging on Salivary Metabolome in Women |
| title_sort | untargeted metabolomic approach to study the impact of aging on salivary metabolome in women |
| topic | LC-HRMS salivary metabolome aging |
| url | https://www.mdpi.com/2218-1989/12/10/986 |
| work_keys_str_mv | AT paulinebosman untargetedmetabolomicapproachtostudytheimpactofagingonsalivarymetabolomeinwomen AT valeriepichon untargetedmetabolomicapproachtostudytheimpactofagingonsalivarymetabolomeinwomen AT anacarolinaacevedo untargetedmetabolomicapproachtostudytheimpactofagingonsalivarymetabolomeinwomen AT laetitialepottier untargetedmetabolomicapproachtostudytheimpactofagingonsalivarymetabolomeinwomen AT jacquesolivierpers untargetedmetabolomicapproachtostudytheimpactofagingonsalivarymetabolomeinwomen AT helenechardin untargetedmetabolomicapproachtostudytheimpactofagingonsalivarymetabolomeinwomen AT audreycombes untargetedmetabolomicapproachtostudytheimpactofagingonsalivarymetabolomeinwomen |