Cisternal versus lumbar cerebrospinal fluid lactate concentration in healthy dogs
The analysis of cerebrospinal fluid biomarkers provides a wide range of information about the neurological health of a patient. Lactate is a metabolic precursor necessary for brain gluconeogenesis. When oxidative impairment or mitochondrial damage is present, lactate alteration occurs. The rostro-ca...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2020-07-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-202007-0002_cisternal-versus-lumbar-cerebrospinal-fluid-lactate-concentration-in-8239-healthy-dogs.php |
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| author | Alba Galan Alejandro Seisdedos-Benzal Beatrice Enrica Carletti Setefilla Quiros Eva Maria Martin David Menor Maria del Mar Granados |
| author_facet | Alba Galan Alejandro Seisdedos-Benzal Beatrice Enrica Carletti Setefilla Quiros Eva Maria Martin David Menor Maria del Mar Granados |
| author_sort | Alba Galan |
| collection | DOAJ |
| description | The analysis of cerebrospinal fluid biomarkers provides a wide range of information about the neurological health of a patient. Lactate is a metabolic precursor necessary for brain gluconeogenesis. When oxidative impairment or mitochondrial damage is present, lactate alteration occurs. The rostro-caudal dynamics of cerebrospinal biomarkers along the craniospinal axis in humans and horses was demonstrated in other studies. To the authors' knowledge, no clinical study has, so far, investigated the cerebrospinal fluid lactate concentration in dogs in association with the puncture site. The purpose of this study was to compare the cerebellomedullary cistern and lumbar cistern cerebrospinal fluid lactate concentrations in healthy dogs. Cerebellomedullary and lumbar cerebrospinal fluids were collected for the cell count, total protein determination and lactate analysis from ten healthy Beagle dogs. The results revealed a significantly increased lumbar cerebrospinal fluid lactate concentration when compared with the cerebellomedullary cistern level. The results included: the total nucleated cell count < 5 cells/µl, the red blood cell count < 500 cell/µl, the total proteins < 0.3 g/l, as well as the cerebellomedullary lactate values (1.44 ± 0.06 mM/l) and the lumbar cistern lactate values (1.58 ± 0.1 mM/l). The results of this study highlight useful data that help to understand the physiological lactate variations depending on the cerebrospinal fluid puncture site. |
| first_indexed | 2024-04-10T07:58:36Z |
| format | Article |
| id | doaj.art-6f86ff98cdab4aca8a6db9fd019e8ff1 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:58:36Z |
| publishDate | 2020-07-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-6f86ff98cdab4aca8a6db9fd019e8ff12023-02-23T03:50:59ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922020-07-0165729730010.17221/136/2019-VETMEDvet-202007-0002Cisternal versus lumbar cerebrospinal fluid lactate concentration in healthy dogsAlba Galan0Alejandro Seisdedos-Benzal1Beatrice Enrica Carletti2Setefilla Quiros3Eva Maria Martin4David Menor5Maria del Mar Granados6Department of Animal Medicine and Surgery, Faculty of Córdoba, University of Córdoba, Córdoba, SpainDepartment of Animal Medicine and Surgery, Faculty of Córdoba, University of Córdoba, Córdoba, SpainSmall Animal Teaching Hospital, University of Liverpool, Liverpool, United KingdomDepartment of Animal Medicine and Surgery, Faculty of Córdoba, University of Córdoba, Córdoba, SpainDepartment of Animal Medicine and Surgery, Faculty of Córdoba, University of Córdoba, Córdoba, SpainDepartment of Animal Medicine and Surgery, Faculty of Córdoba, University of Córdoba, Córdoba, SpainDepartment of Animal Medicine and Surgery, Faculty of Córdoba, University of Córdoba, Córdoba, SpainThe analysis of cerebrospinal fluid biomarkers provides a wide range of information about the neurological health of a patient. Lactate is a metabolic precursor necessary for brain gluconeogenesis. When oxidative impairment or mitochondrial damage is present, lactate alteration occurs. The rostro-caudal dynamics of cerebrospinal biomarkers along the craniospinal axis in humans and horses was demonstrated in other studies. To the authors' knowledge, no clinical study has, so far, investigated the cerebrospinal fluid lactate concentration in dogs in association with the puncture site. The purpose of this study was to compare the cerebellomedullary cistern and lumbar cistern cerebrospinal fluid lactate concentrations in healthy dogs. Cerebellomedullary and lumbar cerebrospinal fluids were collected for the cell count, total protein determination and lactate analysis from ten healthy Beagle dogs. The results revealed a significantly increased lumbar cerebrospinal fluid lactate concentration when compared with the cerebellomedullary cistern level. The results included: the total nucleated cell count < 5 cells/µl, the red blood cell count < 500 cell/µl, the total proteins < 0.3 g/l, as well as the cerebellomedullary lactate values (1.44 ± 0.06 mM/l) and the lumbar cistern lactate values (1.58 ± 0.1 mM/l). The results of this study highlight useful data that help to understand the physiological lactate variations depending on the cerebrospinal fluid puncture site.https://vetmed.agriculturejournals.cz/artkey/vet-202007-0002_cisternal-versus-lumbar-cerebrospinal-fluid-lactate-concentration-in-8239-healthy-dogs.phpbrain energy metabolismcaninepuncturerostro-caudal dynamics |
| spellingShingle | Alba Galan Alejandro Seisdedos-Benzal Beatrice Enrica Carletti Setefilla Quiros Eva Maria Martin David Menor Maria del Mar Granados Cisternal versus lumbar cerebrospinal fluid lactate concentration in healthy dogs Veterinární Medicína brain energy metabolism canine puncture rostro-caudal dynamics |
| title | Cisternal versus lumbar cerebrospinal fluid lactate concentration in healthy dogs |
| title_full | Cisternal versus lumbar cerebrospinal fluid lactate concentration in healthy dogs |
| title_fullStr | Cisternal versus lumbar cerebrospinal fluid lactate concentration in healthy dogs |
| title_full_unstemmed | Cisternal versus lumbar cerebrospinal fluid lactate concentration in healthy dogs |
| title_short | Cisternal versus lumbar cerebrospinal fluid lactate concentration in healthy dogs |
| title_sort | cisternal versus lumbar cerebrospinal fluid lactate concentration in healthy dogs |
| topic | brain energy metabolism canine puncture rostro-caudal dynamics |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-202007-0002_cisternal-versus-lumbar-cerebrospinal-fluid-lactate-concentration-in-8239-healthy-dogs.php |
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