Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase
Microorganisms present in Antarctica have to deal not only with cold temperatures but also with other environmental conditions, such as high UV radiation, that trigger the generation of reactive oxygen species. Therefore, Antarctic microorganisms must have an important antioxidant defense system to...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/1/95 |
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| author | María T. Monsalves Gabriela P. Ollivet-Besson Maximiliano J. Amenabar Jenny M. Blamey |
| author_facet | María T. Monsalves Gabriela P. Ollivet-Besson Maximiliano J. Amenabar Jenny M. Blamey |
| author_sort | María T. Monsalves |
| collection | DOAJ |
| description | Microorganisms present in Antarctica have to deal not only with cold temperatures but also with other environmental conditions, such as high UV radiation, that trigger the generation of reactive oxygen species. Therefore, Antarctic microorganisms must have an important antioxidant defense system to prevent oxidative damage. One of these defenses are antioxidant enzymes, such as catalase, which is involved in the detoxification of hydrogen peroxide produced under oxidative conditions. Here, we reported the isolation and partial characterization of an Antarctic bacterium belonging to the <i>Serratia</i> genus that was resistant to UV-C radiation and well-adapted to cold temperatures. This microorganism, denominated strain I1P, was efficient at decreasing reactive oxygen species levels produced after UV-C irradiation. Genomic and activity assays suggested that the enzymatic antioxidant defense mechanisms of strain I1P, especially its catalase enzyme, may confer UV resistance. This catalase was active in a wide range of temperatures (20−70 °C), showing optimal activity at 50 °C (at pH 7.0), a remarkable finding considering its psychrotolerant origin. In addition, this enzyme was thermostable, retaining around 60% of its activity after 6 h of incubation at 50 °C. The antioxidant defense systems of strain I1P, including its surprisingly thermoactive and thermostable catalase enzyme, make this microorganism a good source of biocompounds with potential biotechnological applications. |
| first_indexed | 2024-12-20T03:00:37Z |
| format | Article |
| id | doaj.art-6f8a90d054ca4017afc690c0bf8805b1 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-20T03:00:37Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-6f8a90d054ca4017afc690c0bf8805b12022-12-21T19:55:45ZengMDPI AGMicroorganisms2076-26072020-01-01819510.3390/microorganisms8010095microorganisms8010095Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable CatalaseMaría T. Monsalves0Gabriela P. Ollivet-Besson1Maximiliano J. Amenabar2Jenny M. Blamey3Fundación Científica y Cultural Biociencia, José Domingo Cañas 2280, Ñuñoa, Santiago 7750132, ChileFundación Científica y Cultural Biociencia, José Domingo Cañas 2280, Ñuñoa, Santiago 7750132, ChileFundación Científica y Cultural Biociencia, José Domingo Cañas 2280, Ñuñoa, Santiago 7750132, ChileFundación Científica y Cultural Biociencia, José Domingo Cañas 2280, Ñuñoa, Santiago 7750132, ChileMicroorganisms present in Antarctica have to deal not only with cold temperatures but also with other environmental conditions, such as high UV radiation, that trigger the generation of reactive oxygen species. Therefore, Antarctic microorganisms must have an important antioxidant defense system to prevent oxidative damage. One of these defenses are antioxidant enzymes, such as catalase, which is involved in the detoxification of hydrogen peroxide produced under oxidative conditions. Here, we reported the isolation and partial characterization of an Antarctic bacterium belonging to the <i>Serratia</i> genus that was resistant to UV-C radiation and well-adapted to cold temperatures. This microorganism, denominated strain I1P, was efficient at decreasing reactive oxygen species levels produced after UV-C irradiation. Genomic and activity assays suggested that the enzymatic antioxidant defense mechanisms of strain I1P, especially its catalase enzyme, may confer UV resistance. This catalase was active in a wide range of temperatures (20−70 °C), showing optimal activity at 50 °C (at pH 7.0), a remarkable finding considering its psychrotolerant origin. In addition, this enzyme was thermostable, retaining around 60% of its activity after 6 h of incubation at 50 °C. The antioxidant defense systems of strain I1P, including its surprisingly thermoactive and thermostable catalase enzyme, make this microorganism a good source of biocompounds with potential biotechnological applications.https://www.mdpi.com/2076-2607/8/1/95antarctica<i>serratia</i>psychrotolerantuv-c radiationcatalasethermostableantioxidantrosoxidative stress |
| spellingShingle | María T. Monsalves Gabriela P. Ollivet-Besson Maximiliano J. Amenabar Jenny M. Blamey Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase Microorganisms antarctica <i>serratia</i> psychrotolerant uv-c radiation catalase thermostable antioxidant ros oxidative stress |
| title | Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase |
| title_full | Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase |
| title_fullStr | Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase |
| title_full_unstemmed | Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase |
| title_short | Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase |
| title_sort | isolation of a psychrotolerant and uv c resistant bacterium from elephant island antarctica with a highly thermoactive and thermostable catalase |
| topic | antarctica <i>serratia</i> psychrotolerant uv-c radiation catalase thermostable antioxidant ros oxidative stress |
| url | https://www.mdpi.com/2076-2607/8/1/95 |
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