IMPACT OF OOCYTE SIZE ON LHRHa INDUCED OVULATION AND FERTILIZED EGG QUALITY IN SADDLED BREAM OBLADA MELANURA (LINNAEUS, 1758)
The objective of this study was to evaluate the effects of oocyte size and luteinizing hormone releasing hormone analogue (LHRHa) on ovulatory success in artificial fertilization. Vitellogenic females with maximum oocyte diameters 400-550 µm were repeatedly injected with LHRHa (20 µg kg-1 per inject...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2013-01-01
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| Series: | Croatian Journal of Fisheries |
| Subjects: | |
| Online Access: | http://ribarstvo.agr.hr/articles/75165_IMPACT_OF_OOCYTE_SIZE_ON_LHRHa_INDUCED_OVULATION_AND_FERTILIZED_EGG_QUALITY_IN_SADDLED_BREAM_OBLADA_MELANURA_(LINNAEUS_1758)_en.pdf |
| Summary: | The objective of this study was to evaluate the effects of oocyte size and luteinizing hormone releasing hormone analogue (LHRHa) on ovulatory success in artificial fertilization. Vitellogenic females with maximum oocyte diameters 400-550 µm were repeatedly injected with LHRHa (20 µg kg-1 per injection). Fish with maximum oocyte diameters <500 µm failed to ovulate. In contrast, all fish with maximum oocyte diameters >500 µm spawned within 48-54 h. These results demonstrate that injected LHRHa is effective for ovulation of saddled bream with maximum oocyte diameters >500µm. |
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| ISSN: | 1330-061X 1848-0586 |